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Effects of peptides in the hypothalamic vasopressinergic nuclei.

Research Project

Project/Area Number 01480142
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionNagoya City University

Principal Investigator

MATSUDA Tomohiro  Nagoya City University, Department of Pharmacology, Professor, 医学部, 教授 (40028361)

Co-Investigator(Kenkyū-buntansha) KAWADE Mayumi  Nagoya City University, Department of Pharmacology, Assistant, 医学部, 助手 (90117862)
TSUSHIMA Hiromi  Nagoya City University, Department of Pharmacology, Assistant, 医学部, 助手 (10080079)
FUJIMOTO Seigo  Nagoya City University, Department of Pharmacology, Associate Professor, 医学部, 助教授 (60079994)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsHypothalamus / Vasopressin / Neuropeptides / Supraoptic nucleus / Paraventricular nucleus / Microinjection / Antidiuresis / ダイノルフィン / 室旁核
Research Abstract

1) Muscarinic agonist, oxotremorine microinjected into the hypothalamic vasopressinergic nucleus (the supraoptic nucleus or the paravetricular nuleus) induced potent antidiuretic effects which were associated with increases in the level of plasma vasopressin. The antidiuretic effects were completely blocked by premicroinjection of muscarinic antagonist, atropine or by preteartment with intravenous injection of vasopressin (V1+V2) receptor antagonist, d (CH2) 5-D-Tyr (Et) -VAVP.
2) Neuropeptide, substance P also caused potent antidiuretic effects, when microinjected into the supraoptic nucleus. At the maximal effects of substance P, vasopressin concentration in the urine increased up to approximately 26-fold the control value. Premicroinjection of substance P receptor antagonist, spantide inhibited the antidiuretic effects of substance P. Substance P microinjected into the paraventricular nucleus also demonstrated potent antidiuretic effects.
3) A kappa-type opioid peptide, dynorphin (1-13) which colocalizes with vasopressin induced potent antidiuretic effects which accompanied in-creases in the plasma level of vasopressin, when injected into the supraoptic nucleus or the paraventricular nucleus. The effects of the supraoptic injection were inhibited by premicroinjection of naloxone, but were not the effects of the paraventricular injection. Dynophin (2-13) which does not bind to opioid receptors induced approximately equipotent effects to dynorphin (1-13) when injected in the supraoptic nucleus, and it induced much less potent effects than dynorphin (1-13), when injected into the paravetricular nucleus.
The results indicate that the stimulation of muscarinic or substance P -receptors in the nuclei by specific agonists induced the release of vasopressin, and that dynorphin (1-13) stimulated the neurons in the paraventricular nucleus through opioid receptors and it stimulated neurons in the supraoptic nucleus via non-opioid receptors, to release vasopressin.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (32 results)

All Other

All Publications (32 results)

  • [Publications] Mayumi Mori,Hiromi Tsushima,and Tomohiro Matsuda: "Effect of vasopressin antagonist on antidiuresis by oxotremorine microinjected into the hypothalamic supraoptic and paraventricularnuclei in a water-loaded and ethanol anesthetized rat." Japan.J.Pharmacol.49. 357-364 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 森 眞由美,津島 宏美,松田 友宏: "視床下部バゾプレッシンニュ-ロン神経核(視索上核)におけるサブスタンスPとそのアナログの作用" 神経化学. 28. 266-267 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiromi Tsushima,Mayumi Mori,and Tomohiro Matsuda: "Effects of fentanyl,injected into the hypothalamic supraoptic and paraventricular nuclei,in a water-loaded and ethanol-anesthetized rat." Neuropharmacology. 29. 757-763 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 森 眞由美,神谷 稔夫,津島 宏美,松田 友宏: "視床下部バゾプレッシンニュ-ロン神経核(視索上核)におけるM_2ームスカリン様レセプタ-によるアセチルコリン遊離調節" 神経化学. 29. 228-229 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 津島 宏美,森 眞由美,松田 友宏: "左右両側、視床下部視索上核間の神経連絡" 神経化学. 29. 262-263 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiromi Tsushima,Mayumi Mori,and Tomohiro Matsuda: "Effects of dynorphin,injected into the hypothalamic supraoptic and paraventricular nuclei,in a water-loaded and ethanol-anesthetized rat." Neuropharmacology. (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 栗山 欣弥,松田 友宏: "脳と神経の薬理(改計2版)" 金芳堂, 262 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mayumi Mori, Hiromi Tsushima, and Tomohiro Matsuda: "Effect of vasopressin antagonist on antidiuresis by oxotremorine microinjected into the hypothalamic supraoptic and paraventricular nuclei in a water-loaded and ethanol-anesthetized rat." Japan. J. Pharmacol.49. 357-364 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mayumi Mori, Hiromi Tsushima, and Tomohiro Matsuda: "Effects of substance P and its analogs in the hypothalamic vasopressinergic nucleus (supraoptic nucleus)." Neurochemistry (Japan). 28. 266-267 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiromi Tsushima, Mayumi Mori, and Tomohiro Matsuda: "Effects of fentanyl, injected into the hypothalamic supraoptic and paraventricular nuclei, in a water-loaded and ethanol-anesthetized rat." Neuropharmacology. 29. 757-763 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tomohiro Matsuda, Hiromi Tsushima, and Mayumi Mori: "Functional roles of opioid receptors in the hypothalamic vasopressinergic nuclei." Transactions of the American Society for Neurochemistry. 21. 297 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mayumi Mori, Toshio Kamiya, Hiromi Tsushima, and Tomohiro Matsuda: "Regulation of release of acetylcholine in the hypothalamic vasopressinergic nucleus (suproptic nucleus) through muscarinic (M2)-receptors." Neurochemistry (Japan). 29. 228-229 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hiromi Tsushima, Mayumi Mori, and Tomohiro Matsuda: "Neuronal connections between the bilateral supraoptic nuclei in the hypothalamus." Neurochemistry (Japan). 29. 262-263 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mayumi Mori,Hiromi Tsushima,and Tomohiro Matsuda: "Effect of vasopressin antagonist on antidiuresis by oxotremorine microinjected into the hypothalamic supraoptic and paraventricular nuclei in a waterーloaded and ethanolーanesthetized rat." Japan.J.Pharmacol.49. 357-364 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] 森 眞由美,津島 宏美,松田 友宏: "視床下部バソプレッシンニュ-ロン神経核(視索上核)におけるサブスタンスPとそのアナログの作用" 神経化学. 28. 266-267 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiromi Tsushima,Mayumi Mori,and Tomohiro Matsuda: "Effects of fentanyl,injected into the hypothalamic supraoptic and paraventricular nuclei,in a waterーloaded and ethanolーanesthetized rat." Neuropharmacology. 29. 757-763 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 森 眞由美,神谷 稔夫,津島 宏美,松田 友宏: "視床下部バゾプレッシンニュ-ロン神経核(神索上核)におけるM_2ームスカリン様レセプタ-によるアセチルコリン遊離調節" 神経化学. 29. 228-229 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] 津島 宏美,森 眞由美,松田 友宏: "左右両側視床下部視索上核間の神経連絡." 神経化学. 29. 262-263 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hiromi Tsushima,Mayumi Mori,and Tomohiro Matsuda: "Effects of dynorphin,injected into the hypothalamic supraoptic and paraventricular nuclei,in a waterーloaded and ethanolーanesthetized rat." Neuropharmacology. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 栗山 欣弥,松田 友宏: "脳と神経の薬理(改訂2版)" 金芳堂, 262 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Mayumi Mori et al: "Effect of vasopressin antagonist on antidiuresis by oxotremorine microinjected into the hypothalamic supraoptic and paraventricular nuclei in a waterーloaded and ethanolーanesthetized rat." Japan.J.Pharmacology. 49. 357-364 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hiromi Tsushima et al: "Effects of fentanyl,injected into the hypothalamic supraoptic and paraventricular nuclei,in a waterーloaded and ethanolーanesthetized rat." Neuropharmacology. 29. 757-763 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 松田 友宏: "神経系の各種レセプタ-:アセチルコリンレセプタ-" Clinical Neuroscience. 8. 18-21 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 森 眞由美ら: "視床下部バソプレッシンニュ-ロン神経核におけるサブスタンスPとそのアナログの作用" 神経化学. 28. 266-267 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 森 眞由美ら: "視床下部バソプレッシンニュ-ロン神経核(視索上核)におけるM_2ームスカリン様レセプタ-によるアセチルコリン遊離調節" 神経化学. 29. 228-229 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 津島 宏美ら: "左右両側視床下部視索上核間の神経連絡" 神経化学. 29. 262-263 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 栗山 欣弥・松田 友宏: "脳と神経の薬理" 金芳堂, 262 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Mayumi Mori: "Effect of vasopressin antagonist on antidiuresis by oxotremorine microinjected into the hypothalamic supraoptic and paraventricular nuclei in a water-loaded and ethanol-anesthetized rat." Japan.J.Pharmacology. 49. 357-364 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hiromi Tsushima: "Effects of fentanyl microinjected into the hypothalamic supraoptic and paraventricular nuclei in a water-loaded and ethanol-anesthetized rat." Neuropharmacology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 松田友宏: "神経系の各種レセプタ-:アセチルコリンレセプタ-" Clinical Neuroscience. 8. 18-21 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 森(河辺)眞由美: "視床下部バソプレッシンニュ-ロン神経核におけるサブスタンスPとそのアナログの作用" 神経化学. 28. 266-267 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 栗山欣弥: "脳と神経の薬理" 金芳堂, 262 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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