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Analysis of the Mechanism of Renal Injury by Application of Monoclonal Antibodies.

Research Project

Project/Area Number 01480163
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNiigata University

Principal Investigator

SHIMIZU Fujio  Niigata University School of Medicine, Professor, 医学部, 教授 (40012728)

Co-Investigator(Kenkyū-buntansha) MORIOKA Tetsuo  Niigata University School of Medicine, Assistant, 医学部, 助手 (00210146)
OITE Takashi  Niigata University School of Medicine, Associate professor, 医学部, 助教授 (60018744)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsMonoclonal Antibody / Proteinuria / Glomerular Epithelial Cell / Mesangium / Mesangiolysis / Cultured Cell / 腎糸球体硬化 / 補体 / slit diaphragm / 腎糸球体上皮細胞 / アミノヌクレオシド腎症
Research Abstract

The purpose of this study is to analyze the mechanism of renal injury at the molecular level by application of monoclonal antibody (MA). We have produced 2 kinds of MAs which induce a massive proteinuria in rats by a single intravenous injection. MA 5-1-6, IgG1, binds to the surface of glomerular epithelial foot processes, mainly to slit diaphragm MA 1-22-3, IgG3, binds to the limited surface of mesangial cells facing endothelial cells. The minimum dose of both MAs required to induce proteinuria is very small in comparison with already reported one in several kinds of experimental glomerulonephritides. This also indicates that each epitope recognized by both MAs is a very limited point which plays the critical role in keeping the normal glomerular permeability.
The study of our MAs indicates that a chain reaction leading to proteinuria with or without mesangial lesions is initiated by the reaction between 2 defined molecules (MA and very specific surface molecules of glomerular cells). In the case of MA 5-1-6 the proteinuria is induced without the involvement of the classical mediators of immune injury such as complement or leukocytes.
We have demonstrated that glomerular cell injury depends on an epitope specific interaction between a cell surface epitope as a receptor and a MA as a ligand. This seems to be an entirely new mechanism by which proteinuria can be induced. The simplicity of MA-induced in vivo models is ideal for analyzing the immunopathology of induced lesions at molecular levels.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Kawachi,H.;Shimizu,F.: "Quantitative study of proteinuria inducing monoclonal antibody 5ー1ー6." Kidney Int.35. 351 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Oite,T.;Shimizu,F.: "Hatenーspecific celluar immune response producing glomerular injury." Clin.exp.Immunol.76. 463-468 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Howie,A.J.;Shimizu,F.: "Different types of sclerosing glomerular lesions in six experimental models of proteinuria." J. Pathol.157. 141-151 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 清水 不二雄: "分子レベルにおける腎障害機序解析の最前線 ーモノクロ-ナル抗体はどこまで有用かー" 医学のあゆみ. 152. 595 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu,F.: "Kinetics of injected proteinuriaーinducing monoclonal antibody and its recognized antigen in rats." J. Am. Soc. Nephrol.1. 539 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okasora,T.;Shimizu,F.: "Altered localization of antigen recognized by proteinuriaーinducing monoclonal antibody in experimental nephrosis." J. Am. Soc. Nephrol.1. 539 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 清水 圧二雄(分担執筆): "臨床腎蔵病学" 朝倉書店, 584 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimzu,F.(contributor): "Structural basis for glomerular dysfunction" Nishimura Co. Ltd., (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Howie, A. J: "Shimizu, F. Different types of sclerosing glomerular lesions in six experimental models of proteinuria." J. Pathol.157. 141 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawachi, H. --: "Shimizu, F. Quantitative study of proteinuria inducing monoclonal antibody 5-1-6." Kidney Int.35,. 351 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] OITE, T. --: "Shimizu, F. Hapten-specific cellular immune response producing glomerular injury." Clin. exp. Immunol.,. 76,. 463, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kagami, S.: "Shimizu, F. Mechanism of formation of subepithelial electron dense deposits in active in situ immune complex glomerulonephritis." Am. J. Pathol.136. 631 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawachi, H.: "Shimizu, F. Induction of proteinuria and morphological changes in the rat by a monoclonal antibody against mesangial cell surface." Kidney Int.,. 37,. 418 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsuyama, M.: "Shimizu, F. Genetic regulation of the development of glomerular sclerotic lesions in the BUF/Mna rat." Nephron. 54. 334 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, F.: "Kinetics of injected proteinuria-inducing monoclonal antibody and its recognized antigen in rats." J. Am. Soc. Nephrol.1. 539 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, F.: "Effects of traditional Chinese medicine on exeperimental nephrosis in rats. In proceedings of the joint meeting 11th international congress of nephrology." Kanebo Pharmaceutical Co Ltd. 17. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Moritaka, T: "Shimizu, F : Production by cultured human monocytes of mesangial cell proliferation factor(s) differing from IL-1 and IL-6" Clin. exp. Immunol.83. 182 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okasora, T.: "Shimizu, F. Altered localization of antigen recognized by proteinuria-inducing monoclonal antibody in experimental nephrosis." Virchows Archiv B cell pathol.60. 41 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato, T.: "Shimizu, F. Nephrotoxic serum nephritis in nude rats : the roles of host immune reactions." Clin. exp. Immunol.84.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Narita, I.: "Shimizu, F. Monocytes secrete factors regulating GAG synthesis in mesangial cells in vitro." Clin. exp. Immunol.84.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, F.: "Analysis of the mechanism of renal injury at molecular levels by application of monoclonal antibodies." proceedings of the IVth Asian Pacific congress of nephrology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, F.: Nishimura Co. Ltd.New concepts of glomerular injury : role of an epitope specific interaction. In structural basis for glomerular dysfunction.,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, F.: Springer-Verlag,. Cell and matrix in monoclonal antibody-induced mesangiolysis. In proceedings of the XIth international congress of nephrology.,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kawachi,H.,Shimizu,F.: "Induction of proteinuria and morphological changes in the rat by a monoclonal antibody against mesangial cell surface." Kidney Int.37. 418 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Matsuyama,M.,Shimizu,F.: "Genetic regulation of the development of glomerular sclerotic lesions in the BUF/Mna rat." Nephron. 54. 334-337 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 清水 不二雄: "分子レベルにおける腎障害機序解析の最前線ーモノクロ-ナル抗体はどこまで有用かー" 医学のあゆみ. 152. 595 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimizu,F.: "Kinetics of injected proteinuriaーinducing monoclonal antibody and its recognized antigen in rats." J.Am.Soc.Nephrol.1. 539 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Morioka,T.,Shimizu,F.: "Production by cultured human monocytes of mesangial cell proliteration factor(s) differing from ILー1 and ILー6." Clin.exp.Immunol.83. 182-186 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Okasora,T.,Shimizu,F.: "Altered localization of antigen recognized by proteinuriaーinducing monoclonal antibody in experimental nephrosis." Virchows Archiv B Cell Pathol. 60. 41-46 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 清水 不二雄(分担執筆): "臨床腎臓病学" 朝倉書店, 584 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimizu,F.(contributor): "Structural basis for glomerular dysfunction" Nishimura Co.Ltd., (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kawachi,H.: "Quantitative study of proteinuria-inducing monoclonal antibody 5-1-6" Kidney International. 35. 351 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 清水不二雄: "単クロ-ン抗体応用による腎障害機序の解析" 日本病理学会会誌. 78(補). 8 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 清水不二雄: "蛋白尿惹起単クロ-ン抗体5-1-6認識抗原の実験腎炎における動態-第2報-" 日本腎臓学会誌. 32. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 清水不二雄: "単クロ-ン抗体応用による腎障害機序の解析" 日本医師会雑誌. 102. 1417 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 清水不二雄: "分子レベルにおける腎障害機序解析の最前線-モノクロ-ナル抗体はどこまで有用か-" 医学のあゆみ. 152. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kawachi,H.: "Quantitative studies of monoclonal antibody 5-1-6-induced proteinuric state in rats" Kidney International.

    • Related Report
      1989 Annual Research Report
  • [Publications] 清水不二雄(分担執筆): "臨床腎臓病学(本田・小磯編)" 朝倉書店, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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