| Project/Area Number |
01480170
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | Yamagata University |
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Principal Investigator |
SENDO Fujiro Yamagata University, Parasitology, Professor, 医学部, 教授 (80091833)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Susumu Yamagata University, Parasitology, Associate Professor, 医学部, 助教授 (50034004)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥6,200,000 (Direct Cost: ¥6,200,000)
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| Keywords | monoclonal antibody / Schistosoma japonicum / Gnathostoma spinigerum / Trichinella spiralis / Trypanosoma cruzi / specific monoclonal antibody / Polyspicific monoclonal antibody / heterophile monoclonal antibody / 單クロ-ン抗体 / 施毛虫 / IgG1 / IgM / Western blotting法 / 交又反応 / モノクロ-ナル抗体 / Western blot法 / IgG_1 / heterophil antibody / 交叉反応 |
|---|
| Research Abstract |
The fundamental tool for diagnosis of parasite infection is egg detection by fecal examination. However, in some parasite infection, it is impossible to detect eggs in the faces because of the specific life history of the worm. Furthermore, in endemic areas, it is sometimes difficult to find specialists skilled in microscopic diagnosis. For these reasons, immuno-sero diagnosis may be a useful approach, but as is well known, crude parasite antigens always show some degree of cross-reactivity with heterologous parasite antigens. To overcome this problem, purification of these antigens is critical. Affinity chromatography using a monoclonal antibody (MoAb) to a specific parasite appears a promising aid to diagnosis. Furthermore, production of recombinant protein using monoclonal antibody as a probe may be useful not only for diagnosis but for the development of a vaccine against parasite infection. With these points in mind, we attempted to establish MoAbs to various helminths as the first step to purification of diagnostic antigens.
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