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Biochemical and Immunological Studies on Pathogenic Factors of Candida Albicans

Research Project

Project/Area Number 01480176
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionYamanashi Medical College

Principal Investigator

FUKAZAWA Yoshimura  Professor, Department of Microbiology, Yamanashi Medical College, 医学部, 教授 (10087760)

Co-Investigator(Kenkyū-buntansha) ARAI Tadashi  President, Biotherapy Research Association, 会長
MIYAKAWA Yozo  Research Associate, Department of Microbiology, Yamanashi Medical College, 医学部, 助手 (00166125)
KAGAYA Keiko  Research Associate, Department of Microbiology, Yamanashi Medical College, 医科部, 助手 (60112984)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1990: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1989: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsCandida albicans / Catalase / Intracellular killing / Adherence / Mannan / Ergosterol / Dimorphism / Antifungal agents / アゾ-ル系抗真菌剤
Research Abstract

We studied the biological and immunological aspects of pathogenic factors of Candida albicans, a main pathogen for candidiasis.
First, we investigated the involvement of catalase in pathogenic factors of the intracellular parasitism of C. albicans. Although catalase production in C. albicans increased by H_2O_2 stimulation, no increase in resistance to killing by neutrophils was observed in the H_2O_2-stimulated yeast cells, indicating that stress-induced catalasre is not a factor of the intracellular parasitism of C. albicans.
Second, we determined the nature of adhesin which is required for adherence of C. albicans to host epitherial cells. Adherence ability of antigen mutants defective in C. albicans serotype A-specific antigen (antigen 6) to epitherial cells markedly reduced as compared with their parent and reverlants. ^1H-nuclear magnetic resonance spectral analyses of the purified mannans from the parent and the mutants, and fractionation of their acetolyzed products revealed that … More the mutant mannan lost mannohexaose (M6) with terminal beta-linkage. The fact that the M6 inhibited the agglutination of C. albicans with antigen 6-specific monoclonal antibody showed the significance of M6 as the determinant for antigen 6. These results suggest that the side chains of M6 specific for C. albicans (A) mannan are largely involved in the adherence mechanisms as ligands. Moreover, such finding was supported finding was supported further by the evidence that adherence ability of antigen 6-possessing Candida species (i. e. C.tropicalis) was higher than that of antigen 6-nonpossessing species (i. e. C. guilliermondii.)
Finally, we investigated the biochemical mechanisms for dimorphism of C. albicans. To determine the mechanism of inhibition of dimorphism, effect of inhibition of various steps in the pathway for ergosterol biosynthesis on dimorphism were examined by several antifungal agents. As a results, it is suggested that inhibition of dimorphism is related to the loss of ergosterol in cytoplasmic membrane. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Y.Miyakawa: "Isolation and chemical biological characterization of antigenic mutants of Candida albicans serotype A" Yeast. 5. 225-229 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮川 洋三: "カンジダ表層抗原と病原性因子" 細胞. 22. 58-62 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y.Miyakawa: "Role of specific determinant of Candida albicans serotype Aーmannan in adherence of Candida species to human buccal cells" Infection and Immunity.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kuribayashi: "Comparative study of Candida adherence to various cell lines of human epitherium" Infection and Immunity.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Miyakawa,: "Isolation and chemical and biological characterization of antigenic mutants of Candida albicans serotype A" Yeast. 5. 225-229 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Miyakawa,: "Role of cell surface antigen in Candida infection" The cell. 22. 58-62 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Miyakawa,: "Role of specific determinant of Candida albicans serotype A-mannan in adherence of Candida species to human buccal cells" Infection and Immunity.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kuribayashi,: "Comparative study of Candida adherence to various cell lines of human epitherium" Infection and Immunity.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 宮川 洋三: "カンジダ表層抗原と病原性因子" 細胞. 22. 58-62 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Miyakawa: "Role of specific determinant of Candida albicans serotype Amannan in adherence of Candida species to human bucal cells" Infection and Immunity.

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kuribayashi: "Comparative study of Candida adherence to various cell lines of human epitherium" Infection and Immunity.

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.MIYAKAWA: "Isolation and Chemical and Biological Characterization of Antigenic Mutants of Candida albicans Serotype A" Yeast. 5. 225-229 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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