| Project/Area Number |
01480179
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Osaka University |
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Principal Investigator |
HONDA Takeshi Res. Inst. for Microb. Dis., Osaka Univ. ; Professor, 微生物病研究所, 教授 (60029808)
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| Co-Investigator(Kenkyū-buntansha) |
YOH Myonsun Res. Inst. for Microb. Dis., Osaka Univ. ; Instructor, 微生物病研究所, 助手 (70093482)
YAMAMOTO Koichiro Res. Inst. for Microb. Dis., Osaka Univ. ; Assoc. Prof, 微生物病研究所, 助教授 (30158274)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1991: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Vibrio Parahaemolyticu / Vp-TDH / Vp-TRH / Hemolysin / Colonization / Monoclonal Antibody / 耐熱性溶血毒 / プロテア-ゼ / モノクロ-ナル抗体 |
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| Research Abstract |
In this study, we investigated the pathogenic mechanism of gastroenteritis due to Vibrio parahaemolyticus. Following results were obtained through this experiment.
1) A Vp-TDH-related hemolysin (named VP-TRH) was discovered as a virulence factor from human isolates of Kanagawa phenoffienon-negative V. parahaemolyticus
2) Production of Vp-TDH/TRH-related toxins by V. choleras non-51, V. mimicus, and V. hollisae was demonstrated. These VP-TDH/TRH family toxins were purified
3) and iharacterized. Gene cloning of these toxins was also performed.
3) Immunological properties of VP-TDH/TRH were analysed by both monoclonal and polyclonal antibodies.
4) Importance of amino acid residues at 60 and 90 position from the N terminus of VP-TDH for. expressing biological. activity was-demonstrated.
5) By preparing chimeric toxins, importance of amino acid residues from 33 to 100 for the heat-stability of Vp-TDH was demonstrated.
6) These chimeric toxins also suggested the importance of two amino acid residues at 89 and 91 for the erythrocyte-specificity.
7) Analysis of mode of action of VP-TDH revealed that VP-TDH acts as a "preforming toxin"
8) Purification and characterization of pili produced by V. parahaemolytiucs were performed.
9) Haemagglutinins of V. parahaemolyticus was demonstrated to be a possible colonization factor of this organism,
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