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Stable Insertion into the Human Cytomegalovirus Genome of a Foreign Gene.

Research Project

Project/Area Number 01480182
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionTokai University

Principal Investigator

WATANABE Yasushi  School of Med., Dept. of Mol. Biol., 医学部, 教授 (60055746)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1990: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsHuman cytomegalovirus / Expression vector / Recombinant virus / Vaccine
Research Abstract

On the bases of previous finding that the HindIII O fragment of 7.8-kb in the genome of human cytomegalovirus strain Towne is nonessential for viral replication, we constructed a vector pKM that directs introduction of foreign genes by homologous recombination precisely replacing the O fragment. Using the vector, we constructed strain Towne-derived recombinant viruses in which a chimeric lacZ gene fused to the SV40 promoter and poly A signals was inserted in place of the O fragment. Two types of recombinants were obtained which carry the chimeric gene in opposite directions. Beta Gal was expressed in HEL cells infected with these recombinants throughout the infection cycle, and the rate of its synthesis in early stages of infection was comparable to that of synthesis of a 65 kDa viral glycoprotein, one of viral proteins expressed abundantly. The chimeric lacZ gene introduced was stable and no lacZ- revertant has been observed so far.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Masataka Takekoshi: "Siteーspecific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter" gene. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 竹腰 正隆: "EB・サイトメガロウイルス" 臨床と微生物. 17. 45-50 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masataka Takekoshi: "Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under the control of the SV40 promoter" Gene. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masataka Takekoshi: "EB・Cytomegalovirus" Clinical Microbiology. 17. 45-50 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masataka Takekoshi: "Siteーspecific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the Sv_<40> premoter" gene. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 竹腰 正隆: "EB・サイトメガロウイルス" 臨床と微生物. 17. 45-50 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takekoshi,Masataka: "Site-specific insertion into the human cytomegalo virus genome of a chimeric lacZ gene fused to the SV40 early promoter." (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 竹腰正隆: "EB・サイトメガロウイルス" 臨床と微生物. 17. 45-50 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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