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Development of an Experimental System by Using Perfused Organs to Study the Combined Effects of Environmental Factors

Research Project

Project/Area Number 01480204
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 公衆衛生学
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TAKANO Takehito  Tokyo Medical and Dental University School of Medicine, Professor, 医学部, 教授 (80126234)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Keiko  Tokyo Medical and Dental University School of Medicine, Research associate, 医学部, 助手 (00211433)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1990: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1989: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsPerfused organ / Redox state / Intracellular respiration / Stress / Nutrition / Organic solvents / Ethanol / Antiーinflammatory drugs / 潅流腎臓 / 腎機能 / 灌流肝臓
Research Abstract

1. When we assess the effect of chemical agents on our health in actual environment, we must consider interractions of their effects. In addition to the interactions between chemical agents, the interactions of chemical agents with some environmental factors including stress and nutrition are also taken into the consideration. We have developed a system consisting of scanning reflectance spectrophotometry and surface fluorometry at the organ level for this purpose. This experimental system enables us to observe the real-time intracellular biochemical changes in perfused rat organs like liver and kidney. 2. Contribution of substrates on eneygy production in perfused rat kidney was studied. L-lactate and alpha-ketoglutarate were efficient substrates for rat kidney to maintain energy metabolism. 3. In the study of effect of stress on trichloroethylene metabolism, it was obtained that 10nM epinephrine increased trichloroethlene metabolism rate in rat liver. 4. Effect of ethanol on styrene metabolism was assessed in the perfused rat liver. Ethanol decreased the rate of styrene uptake in rat liver. And it was suggested that the rate was modified due to changes in nutritional condition.
5. Interactions of flufenamic acid (FA), an anti-inflammatory drug, and ethanol on their metabolisms were studied in the perfused liver from rats under fed and fasted conditions. FA suppressed ethanol oxidation at the concentrations of 0.1mM (2mM of ethanol) that could occur in the actual situation. It was also observed that the effect of FA on the ethanol oxidation was modified by the nutritional condition. An uncoupling effect of FA on renal intracellular energy metabolism was studied in the perfused rat kidney. FA decreased the reduced PN level, the fractional reabsorption of Na, and the fractional reabsorption of Ca. It was suggested that function of energy dependent ion transport systems was influenced because of uncoupling effect of FA surpressing ATP production.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 富田 和久: "エタノ-ル代謝に及ぼすフルフェナム酸(非ステロイド系抗炎症剤)の影響(2)" 日本衛生学雑誌. 45. 304 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 仲田 和代: "アルブミンを使用しないSingleーpassラット潅流賢臓における供給基質の組成について" 日本衛生学雑誌. 45. 554 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Takano: "Validation of a toxicity testing model by evaluating oxygen supply and evergy state in the isolated perfused rat kidney:singleーpass preparation without albumin." Journal of Pharmacological Methods. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K.Tomita: "Effect of an antiーinflammatory drug,fluferamic acid,on ethanol metabolism in the perfused rat liver." Alcoholism. 14. 346 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Kawakami: "Enhanced arrhythmogenicity of freon 113 by hypoxia in the perfused rat heart." Toxicology and Industrial Health. 6. 493-498 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Tomita and T. Takano.: "Effect of a non-steroidal anti-inflammatory drug(flufenamic acid) on ethanol metabolism (2)" Japanese Journal of Hygiene. 45. 304 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Nakata and T. Takano.: "Substrate composition and intracellular energy metabolism in the single-pass perfusion of rat kidney with non-albumin oncotic medium" Japanese Journal of Hygiene. 45. 554 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] K. Tomita and T. Takano.: "Effect of an anti-inflammatory drug, flufenamic acid, on ethanol metabolism in the perfused rat liver" Alcoholism. 14. 346 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Kawakami, T. Takano, and R. Araki.: "Enhanced arrhythmogenicity of freon 113 by hypoxia in the perfused rat heart" Toxicology and Industrial Health. 6. 493-498 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Takano, K. Nakata, T. Kawakami, Y, Miyazaki, M. Murakami, Y. Seo, and E. Suzuki.: "Validation of a toxicity testing model by evaluating oxygen supply and energy state in the isolated perfused rat kidney : single-pass preparation without albumin" J. Pharmacol. Methods.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Takano: "Validation of a foxicity testing model by evaluating oxygen supply and energy state in the isolated perfused rat kidney:singleーpass preparation without albumin." Journal of Pharmacological Methods.

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Tomita: "Effect of an antiーinflammatory drug,flufenamic acid,on ethanol metabolism in the perfused rat liver." Alcoholism. 14. 346 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Kawakami: "Enhanced arrhythmogenicity of freon 113 by hypoxia in the perfused rat heart." Toxicology and Industrial Health.6. 493-498 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 富田和久: "エタノ-ル代謝に及ぼすフルフェナム酸(非ステロイド系抗炎症剤)の影響(2)" 日本衛生学雑誌. 45. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 仲田和代: "アルブミンを使用しないsingle-possラット灌流腎臓における供給基質の組成について" 日本衛生学雑誌. 45. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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