| Project/Area Number |
01480227
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Sapporo Medical College |
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Principal Investigator |
YACHI Akira Sapporo Med. Coll., Dept. Int. Med., Professor., 医学部, 教授 (50045324)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJISAKI Masayuki Sapporo Med. Coll., Dept. Int. Med., Instructor., 医学部, 助手 (50217311)
YABANA Tsuyoshi Sapporo Med. Coll., Dept. Int. Med., Associate Professor., 医学部, 助教授 (70045491)
HINODA Yuji Sapporo Med. Coll., Dept. Int. Med., Instructor., 医学部, 助手 (10165128)
SUGIYAMA Toshiro Sapporo Med. Coll., Dept. Int. Med., Instructor., 医学部, 助手 (00196768)
IMAI Kohzoh Sappro Med. Coll., Dept. Int. Med., Assistant Professor., 医学部, 講師 (60117603)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1989: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Campylobacter pylori / Helicobacter pylori / Chronic Gastritis / Monoclinal antibody / Local immune response / Campylobacter pylori / 胃潰瘍 / 十二指腸潰瘍 / 組織学的重症度 |
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| Research Abstract |
Campylobacter pylori or Helicobacter pylori has recently been implicated as an etiologic agent of gastroduodenal disorders. Comparing the antibody to Helicobacter pylori in the sera of patients to that of normal controls by Western blot, a unique antibody was detected in the sera of patients, which reacted with the 25 Kilo molecular weight antigen of Helicobacter pylori. On the other hand, monoclinal antibody CP3 which was prepared in our laboratory also recognized the 25 Kilo molecular weight antigen of Helicobacter pylori. We then examined whether the serum antibody of the patient recognized the CP3 antigen purified by monoclinal antibody CP3. Western blot analysis demonstrated that the patient's serum strongly reacted with the affinity-purified CP3 antigen. By using monoclinal antibody CP3, We have established an enzyme-linked immunosorbent assay to detect CP3 antibody in sera. In chronic gastritis and gastric ulcer patients, the titer of CP3 antibody was significantly higher than that of normal controls and correlated with the histological grade of antral gastritis. The detection of CP3 antibody in sera is useful to diagnose chronic gastritis and gastric ulcer associated with Helicobacter pylori infection and also to evaluate the grade of gastritis.
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