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Pathogenetic Significance of Serum Glycoprotein Abnormality in Alcoholic Liver Injury.

Research Project

Project/Area Number 01480231
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionKanazawa Medical University

Principal Investigator

TAKADA Akira  Division of Gastroenterology, Department of Internal Medicine, Professor., 医学部, 教授 (30064497)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥5,700,000 (Direct Cost: ¥5,700,000)
KeywordsAlcoholic liver injury / Pathogenesis / mechanism of development / Glycoproteins / Microheterogeneity / Transferrin / Retention in hepatocytes / Golgi apparatus / 病因論 / 等電点電気泳動 / シア-ル酸 / アルコ-ルピラゾ-ル肝炎 / トランスフェリン / 糖蛋白の分泌障害 / glycosilationの障害
Research Abstract

Both in human and rat alcoholic liver injury (ALI), transferrin (TF) was strongly stained in the ballooned hepatocytes. This change was related to the appearance of microheterogeneity (MCHT) of serum TF. By immunoelectron microscopy, Tf retained in the Golgi apparatus. Incorporation rate of ^<14>C-fucose and ^3H-leucine, especially fucose, to serum Tf decreased, and secretion of these labels from the Golgi apparatus delayed in ALI. By the Pulse-chase labeling analysis in cultured hepatocytes using ^<35>S-methionine, it was suggested that maturation of Tf may be retarded in ALI. MCHT of serum Tf and alphal-antitrypsin, but not other glycoproteins, was clearly detected by Western blotting. MCHT disappeared by the treatment of sialidase, indicating that MCHT of TF was caused by changes in silalic acid. In severe liver disease, such as decompensated liver cirrhosis, MCHT of TF was also found. However, the isoelectric focusing pattern of such disease was different from that of ALI. The differences was found even in the sialic acid-treated serum, suggesting that MCHT of Tf may not be related to the decrease of asialo-glycoprotein receptor on the hepatocyte membrane. A simple method to detect MCHT of serum TF was developed. Using this method, MCHT of TF was found in 73 % of 100 patients with ALI. MCHT was more frequently found in severe type of ALI. However, MCHT of TF could not found in any case of alcoholic pancreatitis and heavy drinkers without liver disease. These results suggest that MCHT is a marker of alcohol specific liver injury, but not chronic alcoholism.

Report

(3 results)
  • 1991 Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Yoshiro Matsuda: "Effects of ethanol on the secretion of hepatic secretory protein in rat alcoholic liver injury." Alcohol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiro Matsuda: "Accumulation of glycoprotein in the golgi apparatus of the hepatocytes in alcoholic liver injuries." American Journal of Gastroenterology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高田 昭: "アルコ-ル性肝障害における肝分泌性糖蛋白異常の病因論的意義." 病態生理. 9. 915-920 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高田 昭: "わが国におけるアルコ-ル性肝障害の病態" 日本消化器病学会雑誌. 88. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 王 健松: "アルコ-ル性肝障害の生化学マ-カ-の分析ー特に血清transferrinの微小変異の意義ついて." アルコ-ル代謝と肝.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiro Matsuda, Akira Takada, et al.: "Effects of ethanol on the secretion of hepatic secretory protein in rat alcoholic liver injury." Alcohol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiro Matsuda, Akira Takada, et al.: "Accumulation of glycoprotein in the Golgi apparatus of the hepatocytes in alcoholic liver injury." Am. J. Gastroenterol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akira Takada: "The pathogenetic significance of serum glycoprotein abnormality in alcoholic liver injury." Byotai Seiri (Pathophysiology). 9. 915-920 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Akira Takada: "Pathophysiologic features of alcoholic liver injury in Japan." Jpn J Gastroenterol. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Jian-Song Wang, Akira Takada, et al.: "Studies on the biochemical markers of alcoholic liver injury ; especially to the significance of microheterogeneity of serum transferrin."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 高田 昭: "アルコ-ル性肝障害における肝分泌性糖蛋白異常の病因論的意義." 病態生理. 9. 915-920 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高田 昭: "わが国におけるアルコ-ル性肝障害の病態" 日本消化器病学会雑誌. 88. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 王 健松: "アルコ-ル性肝障害の生化学マ-カ-の分析ー特に血清transferrinの微小変異の意義について." アルコ-ル代謝と肝.

    • Related Report
      1990 Annual Research Report
  • [Publications] Yoshiro Matsuda,Akira Takada,et al.: "Effects of ethanol on the secretion of hepatic secretory protein in rat alcoholic liver injury." ALCOHOLISM:Clinical and Experimental Reseach.

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshiro Matsuda,Akira Takada,et al.: "Accumulation of glycoprotein in the golgi apparatus of the hepatocutes in alcoholic liver injuries." The American Journal of Gastroenterology.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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