• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Activation and differentiation of eosinophils in asthma

Research Project

Project/Area Number 01480234
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Respiratory organ internal medicine
Research InstitutionChiba University

Principal Investigator

KOIKE Takao (1991)  Chiba Univ., Medicine Lecturer, 医学部, 講師 (80146795)

岩本 逸夫 (1990)  千葉大学, 医学部, 助手 (10111436)

富岡 玖夫 (1989)  千葉大学, 医学部, 助教授 (20009632)

Co-Investigator(Kenkyū-buntansha) TAKABAYASHI Katsumi  Chiba Univ., Medicine Lecturer, 医学部, 助手 (90188079)
IWAMOTO Itsuo  Chiba Univ., Medicine Lecturer, 医学部, 助手 (10111436)
TOMIOKA Hisao  Toho Univ., Medicine Professor, 医学部, 教授 (20009632)
小池 隆夫  千葉大学, 医学部, 助手 (80146795)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsBronchial asthma / Eosinophil / Anti-eosinophil antibody / Cytokine / Substance P / Eosinophilia / ロイコトリエン / 化学伝達物質 / 抗アレルギ-薬 / 神経ペプチド / ヒト好酸球 / 低親和性IgEレセプタ- / 抗好酸球抗体
Research Abstract

In the first series of experiments, we have developed and characterized an anti-eosinophil monoclonal antibody AE500 that had been raised by im = izing mice with blood eosinophils from patients with hypereosinophilic syndron-c. AE500 selectively reacted with blood eosinophils and neutrophils from patients with blood eosinophilia, but not from normal subjects. The reactivity of AE500 with eosinophils was induced in vitro by incubating the eosinophils with GM-CSF, but not with IL-3 or IL-5. These findings suggest that the anti-eosinophil monoclonal antibody AE500 recognizes a molecule that is expressed on eosinophils and neutrophils which have been activated by GM-CSF in vivo. In the second series of experiments, we examined the effects of cytokines on the differentiation of eosinophils from the progenitors in human cord blood. Mature eosinophils were obtained after a 4-week culture of cord blood mononuclear cells with IL-3 and IL-5. INF-r decreased the eosinophil differentiation by greater than 90%. IL-2 and IL-4 also decresed the eosinophil differentiation, but increased the number of T lymphocytes. These results suggest that IFN-r, IL-2 and IL-4 negatively regulate the differentiation of human eosinophils from the progenitors. Finally, we examined the effect of a neuropeptide substance P on the activation of human= eosinophils. Substance P caused superoxide generation and ECP release in a dose-dependent manner, the effect of which was mediated by the C-terminal peptide. In conclusion, our results indicate that cytokines and neuropeptides regulate the activation and differentiation of human eosinophils.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 岩本 逸夫: "未梢血液中における好酸球の動態" 炎症. 10. 375-378 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 落合 賢一: "好酸球増多症の顆粒球を認識するモノクロ-ナル抗体の確立" アレルギ-. 39. 459-464 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中川 典明: "Substance Pによるヒト好酸球活性化の機序" アレルギ-. 39. 1142 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ochiai,K.: "Characteristics of an anti-eosinophil monoclonal antibody that recognizes granulocytes from patieuts with blood eosinophilia but not from subjects witout eosinophilia" Clin.Exp.Immunol. 84. 283-288 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamoto,I.: "The effect of cytokines on IL-3 and IL-5-induced eosinophil differentiation from cord blood mononuclear cells" J.Allergy Clin.Immunol.87. 349 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩本 逸夫: "好酸球活性化におけるサイトカインの役割" 呼吸. 10. 1096-1101 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamoto I.: "Analysis of heterogeneity of human eosinophils." Jpn. J. Inflammation. 10. 375-378 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ochiai K.: "An anti-eosinophil monoclonal antibody AE500 that reacts only with granulocytes from hypereosinophilic patients." Jpn. J. Allergology. 39. 459-464 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakagawa N.: "Mechanism of activation of human eosinophils by substance P." Jpn. J. Allergology. 39. 1142 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ochiai K.: "Characteristics of an anti-eosinophil monoclonal antibody that recognizes granulocytes from patients with blood eosinophilia but not from subjects without eosinophilia." Clin. Exp. Immunol.84. 283-288 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamoto I.: "The effect of cytokines on IL-3 and IL-5-induced eosinophil differentiation from cord blood mononuclear cells." J. Allergy Clin. Immunol.87. 349 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Iwamoto I.: "Role of cytokines in eosinophil differentiation and activation." Kokyu. 10. 1096-1101 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩本 逸夫: "好酸球活性化におけるサイトカインの役割" 呼吸. 10. 1096-1101 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwamoto,I.: "The effect of cytokines on ILー3 and ILー5ーinduced eosinophil differentiation from cord blood mononuclear cells." J.Allergy Clin.Immunol. 87. 349 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 川島 敏男: "新しい抗アレルギ-薬TBXのヒト好酸球からのChemical mediator遊離抑制作用" アレルギ-. 40. 1121 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 岩本 逸夫: "臍帯血単核球の好酸球分化に対するILー2、ILー4およびIFNーγの効果" 好酸球1990. 94-95 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 冨岡 玖夫: "新しい抗アレルギ-薬Lー705の好酸球・好中球活性化に対する抑制作用" 好酸球1990. 4-5 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ochiai,K.: "Characteristics of antiーeosinophil monoclonal antibody that recognizes granulocytes from patients with blood eosinophilia but not from subjects without eosinophilia" Clin.Exp.Immunol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Iwamoto,I.: "The effect of cytokines on ILー3 and ILー5ーinduced eosinophil differentiation from cord blood mononuclear cells" J.Allergy Clin.Immunol.87. 349 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 中川 典明: "Neuropeptidesと白血球活性化 2.Substance Pによるヒト好酸球活性化の機序" アレルギ-. 39. 1142 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 落合 賢一: "好酸球増多症の頼粒球を認識するモノクロ-ナル抗体の確立" アレルギ-. 39. 459-464 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takabayashi.,K.,Kioke,T.,Kurasawa,K.,Matsumura,R.,Sato,T.,Tomioka,H.,Ito,I.,Yoshiki.T.,and Yoshida.,S.: "Effect of FK-506,a novel immunosuppressive drug on murine SLE." Clin.Immunol.Immunopathol.,. 51. 110-117 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Iwamoto,I.,Tanaka,M.,Ra,C.,Tomioka,H.,Yoshida,S.,Fujita,Y.and Inoue,K.: "Differentiation in Dose-Resposne Curves of Airway Responsiveness to Inhaled Methacholine Between Asthmatics and Nonasthmatics." J.Asthma.26. 291-298 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 富岡玖夫: "抗IgE自己抗体" アレルギ-. 38. 305-313 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 富岡玖夫: "気管支喘息における炎症論とMejor Basic Protein(MBP)." フアルマシア. 26. 41-45 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Koike,T.,Tsutsumi,A.,Nawato,Y.,Tomioka,H.Yoshida,S.,: "Prevalence and Role of IgG anti-IgE Autoantibody in Allergic Disorders.Monogr Allergy.vol.26,Autoantibodies to to Immunoglobulins" P.Dukor,L.A.Hanson,P.Kallos,F.Shakib Karger(Basel), 274 (1989)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi