| Project/Area Number |
01480248
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka University |
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Principal Investigator |
INOUE Michitoshi Osaka University Hospital, Department of Medical Information Science, Professor, 医学部・附属病院, 教授 (30028401)
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| Co-Investigator(Kenkyū-buntansha) |
CHIHARA Kunihiro Osaka University, Faculty of Engineering Science, 基礎工学部, 助教授 (80029561)
UEMATSU Masaaki Osaka University Hospieal Clinical Feffon, 医学部・附属病院, 医員
TANOUCHI Jun Osaka University School of Medicine, Assistant, 医学部, 助手 (20197544)
HORI Masatsugu Osaka University School of Medicine, Assistant, 医学部, 助手 (20124779)
TAKEDA Hiroshi Osaka University Hospieal, Department of Medical Information Scinece, Assistant, 医学部・附属病院, 助教授 (20127252)
北畠 顕 大阪大学, 医学部, 講師 (00124769)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥4,100,000 (Direct Cost: ¥4,100,000)
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| Keywords | Atherosclerosis / Shear Stress / Doppler Velocimeter / Endothelial Cells / Microfilaments / Hypercholesterolemia / Intimal Lesions / atherosclerosis / endothelial cells / Shear Stress / Doppler / hypercholesterolemia |
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| Research Abstract |
To interrelate low shear stress conditions in vivo, endothelial microfilament bundle formation and intimal plaque formation, we introduced a mild aortic stenosis in hypercholesterolemic fat-fed beagles. Shear stress was calculated from the instantaneous velocity profiles reconstructed from the data obtained with a 80 channel 20 MHz pulsed Doppler velocimeter. The amount of endothelial microfilament bundles was evaluated as the ratio of the integrated area filled with microfilaments to the total area of the endothelial cell (F/C) on transmission electron photomicrographs. Three sites were compared : 10 mm proximal to the stenosis (PROX), immediately upstream from the the stenosis (ST) and immediately distal to the stenosis (DISY). Shear stress was increased at ST and decreased at DIST as compared with PROX. A positive correlation was noted between shear stress and F/C (r=0.94, p<0.01). Intimal plaques were formed exclusively immediately distal to the stenosis, where shear stress was significantly low. We have also developed an in vitro shear stress loading instrument to study the alterations in various cell responses under variable shear stress conditions. Thus, low shear stress conditions may attenuate endothelial microfilament bundle formation and may promote the formation of intimal plaques under atherogenic conditions.
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