• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cytokine Status and Susceptibility to Specified Pathogens in Human Neonates and in Recipients of Marrow Grafts

Research Project

Project/Area Number 01480257
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pediatrics
Research InstitutionKanazawa University

Principal Investigator

TANIGUCHI Noboru  Kanazawa Univ. Sch. of Med. Professor, 医学部, 教授 (10019888)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1990: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1989: ¥4,100,000 (Direct Cost: ¥4,100,000)
KeywordsCord blood / Cytokines / Very low birth weight infants / Bone marrow transplant / CD45 antigens / Naive T cells / Memory T cells / CD2-mediated proliferation / CD45抗原ファミリ- / モノカイン / リンホカイン / 新生児 / インタ-ロイキン-6 / 細菌刺激
Research Abstract

Several kinds of cytokines were identified in blood plasma when heparinized whole blood was stimulated directly with various stimuli, such as bacteria, bacterial products, and lectins. Immunohistologic and in situ hybridization studies confirmed that IL-1, IL-6, and EFN-gamma were produced almost exclusively by stimulated monocytes in this whole blood system. IL-6 producing ability of cord blood from neonates with over 1,800 gm of birth weight was comparable to adult controls, while very low birth weight infants born at less than 30 weeks of gestation weighing less than 1,500 gm showed poor ability to produce IL-6.
It has been claimed that isoform expression patterns of CD45 antigen family denote mutually exclusive two T cell subsets : "naive" T cells expressing CD45RA^+, RO^- and "memory T cells with CD45RO^-, RO^+ phenotype. Almost all T cells in cord blood expressed naive phenotype. Relative proportions of circulating memory T cells increased with advancing age and reached adult leve … More ls around 12 years of age.
By using reverse transcriptase/PCR technology, it was confirmed that CD4^+CD45RO^- naive T cells could not express mRNAs for IL-4, IL-5, and IFN-gamma on stimulation. CD4^+, CD45RO^+ memory T cells had the ability to produce these lymphokines. In addition, CD4^+ naive T cells as well as whole cord CD4^+ T cells exerted no significant help for B cell differentiation, while CD4^+ memory T cells acted as an efficient helper in PWM- stimulated coculture system. In vitro stimulation of CD4^+ naive T cells and whole cord CD4^+ T cells with PHA in the presence of exogenous IL-2 caused phenotypic conversion from CD45RO^- to CD45RO^+. Following phenotype conversion, originally naive CD4^+ T cells acquired some memory T cellーlike function.
FACS-purified CD4^+ naive T cells could not respond to a combination of anti-CD2 antigens in the absence of monocytes, whereas CD4^+ memory T cells showed vigorous proliferation. CD4^+ memory T cells, but not CD4^+ naive T cells, could produce IL-6 in response to anti-CD2. Whether IL-6 was produced or not seems to be crucial for anti-CD-2mediated cellular proliferation. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kato,K.: "Detection by in situ hybridization and phenotypic characterization of cells expressing ILー6 mRNA in human stimulated blood." JournaL of Immunology. 144. 1317-1322 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yachie,A.: "The capability of neonatal leukocytes to produce IL 6 on stimulation assessed by whole blood culture." Pediatric Reserch. 27. 227-233 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyawaki,T.: "Differential expression of CD45RO(UCHL 1) and its functional relevance in two subpopulations of circulating TCR-S/8^+ lymphocytes." Journal of Experimental Medicine. 171. 1833-1838 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yokoi,T.: "EpsteinーBarr virusーimmortalized B cells produce ILー6 as an autocrine growth factor." Immunology. 70. 100-105 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara,Y.: "Role of interleukin 6 for differential responsiveness of naive and memory CD4^+ T cells in CD2ーmediated activation." Journal of Experimental Medicine. 172. 1419-1424 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taga,K.: "Preferential expression of ILー2 receptor subunits on memory populations within CD4^+ and CD8^+ T cells." Immunology. 72. 15-19 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kato, K. et al.: "Detection by in situ hybridization and phenotypic characterization of cells expressing IL-6 mRNA in human stimulated blood." Journal of Immunology. 144. 1317-1322 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yachie, A. et al.: "The capability of neonatal leukocytes to produce IL-6 on stimulation assessed by whole blood culture" Pediatric Research. 27. 227-133 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miyawaki, T. et al.: "Differential expression of CD45RO (UCHL I) and its functional relevance in two subpopulations of circulating TCR gamma/delta^+lymphocytes." Journal of Experimental Medicine. 171. 1833-1838 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yokoi, T. et al: "EpseinーBarr virusーimmortalized B cells produce IL-6 as an autocrine growth factor." Immunology. 70. 100-105 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kasahara, Y. et al: "Role of interleukin 6 for differential responsiveness of naive and memory CD4^+ T cells in CD2-mediated activation." Journal of Experimental Medicine. 172. 1419-1424 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Taga, K. et al.: "Preferential expression of IL-2 receptor subunits on memory populations within CD4^+ and CD8^+ T cells." Immunology. 72. 15-19 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yachie,A.et al.: "The capability of neonatal leukocytes to produce IL 6 on stimulation by whole blood" Pediatric Research. 27. 227-233 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Miyawaki,T.et al.: "Differential expression of CD45RO(UCHL 1) and its functional relevance in two subpopulations of circulating TCRー lymphocytes" Journal of Experimental Medicine. 171. 1833-1838 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 笠原 善仁: "ヒト新生児CD4抗原陽性T細胞の機能的分化誘導におけるCD45抗原の意義" 金沢大学+金医学会雑誌. 99. 749-764 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yokoi,T,et al.: "EpsteimーBarr virusーimmortalized B cells produce ILー6 as an autocrine growth factor" Immunology. 70. 100-105 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kasahara,Y.et al.: "Role of interleukin 6 for differential responsiveness of naive and memory CD4^+ T cells in CD2ーmediated activation" Journal of Experimental Medicine. 172. 1419-1424 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Taga,K.et al.: "Preferential expression of ILー2 receptor subunits on memory populations within CD4^+ and CD8^+ T cells" Immunology. 72. 15-19 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Saikawa,Y.et al.: "Interelukin 3 enhanced interleukin 2-dependent maturation of NH progenitor cells in bone marrow from mice with severe combined immunodeficiency." Cellular Immunology. 124. 38-49 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yachie,A.et al.: "Developmental changesoof double negative(CD3^+,4^-,8^-)T cells in human peripheral blood." Clinical and Experimental Immunology. 76. 258-261 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kato,K.et al: "Detection by insitu hybridization and phenotypic characterization of cells expressing IL-6 mRNA in humanstimulated blood." Journal of Immunology. 144. 1317-1322 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yachie,A.et al.: "The capacity of neonatal leukocyte to produce IL-6 on stimulation by whole blood." Pediatric Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Miyawaki,T.et al.: "Differential expression and its functional relevance of CD45RO(UCHL-1)in two subpopulations of circulating TCR-γ/δ lymphocytes." Journal of Experimental Medicine. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yokoi,T.et al.: "Epstein-Barr virus-immortalized B cells produce interleukin 6 in an autocrine manner." Immunology. (1990)

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi