| Project/Area Number |
01480268
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Kyorin University School of Medicine |
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Principal Investigator |
SHIOHARA Tetsuo Kyorin Univ. Sch. of Med. Associate Professor, 医学部, 助教授 (10118953)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Masaru Kyorin Univ. Sch. of Med. Assistant Professor, 医学部, 講師 (30142417)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥4,500,000 (Direct Cost: ¥4,500,000)
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| Keywords | Epidermotropism / T-cell clones / LFA-1 / Thy 1^+ epidermal cells / Thymus dependency / Thy1^+epidermal cell / 胸腺依存性 / Thy1^+ EC / サイトカインレセプタ- / 表皮由来サイトカイン / ILー6 / GMーCSF / Cell adhesion nolecule |
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| Research Abstract |
We previously established murine T cell clones with special tropism the epidermis both in vitro in vivo. Using these T cell clones, we attempted to elucidate the mechanisms by which certain T cells preferentially migrate to the epidemis.
1. To determine which cell adhesion molecules are involved in epidermotrpoism of T cells, the expression of rariaus T-cell surface molecules on epidermotropic and non-epidermotropic T cell clones was examined by flow cytometric analysis. The results showed that LFA-1 was preferentially expressed on the surface of epidermotropic T cell clones, while non-epidermotropic T cell clones were negative or weakly positive for LFA-1.
2. These epidermotropic T cells showed directional migration toward the epidermis in an in vitro migration assay under agar gel. Antibody inhibition studies demonstrated that of various mAb to T-cell surface molecules only anti-LEA-1 mAb completely inhibited the in vitro migartion of the T cells toward the epidermis. These findings indicate that the LFA-1 also plays an important role in directing T cell migration to the epidermis.
3. It was found that epidermal structures that had spontaneously recovered from the destruction induced by inteademal injection of the epidermotropic T cells became resistant to subsequent attempts to induce the destruction by the T cells. This resistance correlated well with the great increase in Thy 1^+ epidermal cell numbers. The resistance was never induced in athymic nude mice and adult thymectomized mice. The expansion of the Thy 1^+ EC that mature in thymus would be crucial to the induction of the resistance.
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