| Project/Area Number |
01480274
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Osaka University |
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Principal Investigator |
KOZUKA Takahiro Osaka University, Radiology, Professor, 医学部, 教授 (40028478)
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| Co-Investigator(Kenkyū-buntansha) |
HARADA Koshi Osaka University, Radiology, Asst. Professor, 医学部, 講師 (70156503)
浜田 星紀 大阪大学, 医学部, 助手 (80198803)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1991: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | MRI / ICP / Contrast Media / metallo-porphyrin / Atherosclerosis / 磁気共鳴画像 / 動脈硬化 / 家兎 / 金属ポルフィリン |
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| Research Abstract |
The present study was undertaken to determine whether MN (III) complex of tetrakis (4-sulfonatophenyl) porphyrin (Mn-TSPP) and Mn (III) complex of tetrakis (4-carboxyphenyl) Porphyrin (Mn-TCPP) could be used to reveal the atheroma and sortie tissue in fifteen male New Zealand White rabbits by means of quantitative analysis employing 4.7T MRI and ICP analysis. Compared with control group, the same significant enhancement of noninvolved tissue were demonstrated in both receiving groups (0.050 mmol/kg of MN-TSPP and 0.125 mmol/kg ot' MN-TCPP) at 48 hours after intravenous administration with ex vivo Tl weighted images. Both Mn-complexes failed to enhance the atheromatous lesions. However, ICP analysis revealed the same distribution of manganese between the atheroma and the noninvolved tissues. The atheromatous tissue can be theoretically divided into the extracellular space and the intracellular one. If these agents can distribute into the intracellular space of atheroma, the abundant free-water should commit the enhancement (Tl shortening mechanism). However, MRI results revealed no-enhancement of atheroma. Therefore, MRI and ICP results implicated that Mn-porphyrins distributed into the extracellular space of atheroma. Based on these consideration an appropriate contrast agent depicting atheroselerosis should enter the intracellular space of atheromatous lesions. These characteristics and an adequate combined screening system which consist of MRI and ICP analysis were established in this report.
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