Grant-in-Aid for General Scientific Research (B)
RNAs derived from human and mouse hypothalami were reversetranscrived to each cDNA. Using each cDNA as a temprate and primers corresponding to the sequence of cDNA encoding rat proTRH, polymerase chain reaction was performed. The resulted cDNA was subcloned into pGEM3Z vector and the sequence determined. The human proTRH cDNA was transfected into CHO cells and resulted proteins were analyzed for proTRH using the specific anti-proTRH antibody. Restriction fragment length polymorphism using the human proTRH cDNA showed no significant difference in DNAs of normal control subjects and patients with TRH-deficiency and dpression.
Attribution of KAKENHI