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Analysis and suppression of Graft-versus-Host (GVH) reaction after bone marrow transplantation

Research Project

Project/Area Number 01480296
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionUniversity of Tokyo

Principal Investigator

MINAMI Matsuhiko  University of Tokyo, Dept Transfusionn Medicine, Associate Professor, 医学部(病), 助教授 (60092342)

Co-Investigator(Kenkyū-buntansha) TAKIGUCHI Masafumi  University of Tokyo, Dept Immunology, Assistant Professor, 医科学研究所, 助手 (00183450)
JUJI Takeo  University of Tokyo, Dept Transfusionn Medicine, Professor, 医学部(病), 教授 (20009997)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1990: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1989: ¥5,000,000 (Direct Cost: ¥5,000,000)
KeywordsGVH / MHC / minor histocompatibility / CTL / HLA / T cell receptor / alloreactive T cell / 細胞障害性T細胞 / イディオタイプ / Adhesion molecule / CD2 / ICAM-1 / トランスジェニックマウス
Research Abstract

The project suppored by this grant intended to clarify mechanisms of induction of graft-versus-host (GVH) diseases. The clarification is required for establishment of methods for prevention or therapy of GVH diseases. We did the following subjects ; 1) analysis of major histocompatibility complex (MHC) molecules, especially HLA class I molecules, 2) analysis of minor histocompatibility (mH) antigenic system in human, and 3) finally suppression of alloreactive T cells inducing GVH diseases. First, we analyzed molecular structure and molecular evolution on HLA-B51 and B52, HLA-B35 and HLA-C molecules. Further, from the peripheral blood lymphocytes of a patient with kidney transplantation, we established cytotoxic T cell clones responding to a type of mH antigen on the cells of the patient. The CTL clones could kill HLA-B35-positive cells without expression of the type of mH antigen in the presence of the peptide (s) eluted from the cells of the patient. Also, from the transfused patient we established T cell clone reacting to mH antigen in a HLA-DR9-restricted manner. Finally, in the serum of a multitransfused patient we detected and characterized antibodies reacting to T cell receptors expressed on the T cells reacting to allo-HLA expressed on the patient.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Matsumoto,K.,Yamamoto,J.,Hiraiwa,M.,Kano,K.,and Takiguchi,M.: "Discrimination of HLA-B5 crossreactive group antigens by human allospecific CTL clones." Transplantation. 49. 1164-1167 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kariyone,I.,Tanabe,M.,Jiji,T.,Kano,K.,and Takiguchi,M.: "Functional expression of HLA-C black antigens on human blood lymphocytes." Journal of Immunology. 145. 3714-3718 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto,J.,Hiraiwa,M.,Hayashi,H.,Tanabe,M.,Kano,K.,and Takiguchi,M.: "Two amino acid substitutions at residues 63 and 67 between HLA-B51 and HLA-Bw52 from multiple epitopes recognized by allogeneic T cells." Immunogenetics. 33. 286-289 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura,M.,Sakai,K.,Akaza,T.,Mitomi,Y.,Nieda,M.,Minami,M.,and Juji,T.: "Anti-idiotypic antibody to T cell receptor in multiply transfused patients may play a role in resistance to graft-versus-host disease." Transfusion. 32. 719-728 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura,M.,Sakai,K.,Akaza,T.,Mitomi,Y.,Nieda,M.,Minami,M.,and Juji,T.: "Establishiment of human minor histocompatibility antigen-specific cytotoxid T cell clones restricted by HLA-DR9." Transplantation. 55. 1181-1186 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanabe,M.,Minami,M.,Kano,K.and Takiguchi.M.: "The allorecognition of H-2k^6-specific CD_4-CD_8-T cell hybridomas is influenced by the substitution at reside 256 of MHC class I motecules." J.Immunol.145. 1968-1973 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ymanoto,J.,Kariyone,I.,Akiyama,N.,Kano,K.,and Takiguchi,M.: "Presentation of human minor histocompatibility antigens by HLA-B35 and HLA-B38 mo" Proc.Natl.Acad.Sci.USA. 87. 2583-1587 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tanabe, M., Minami, M., Kano, K., and Takiguchi, M.: "The allorecognition of H-2K^b-specific CD4^-CD8^-T cell hybridomas is influenced by the substitution at residue 256 of MHC class I molecules." J.Immunol.145. 1968-1973 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ymamoto, J., Kariyone, I., Akiyama, N., Kano, K., and Takiguchi, M.: "Presentation of human minor histocompatibility antigens by HLA-B35 and HLA-B38 molecules." Proc.Natl.Acad.Sci.USA.87. 2583-2587 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Matsumoto, K., Yamamoto, J., Hiraiwa, M., Kano, K., and Takiguchi, M.: "Discrimination of HLA-B5 crossreactive group antigens by human allospecific CTL clones" Transplantation. 49. 1164-1167 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kariyone, I., Tanabe.M., Juji, T., Kano, K., and Takiguchi, M.: "Functional expression of HLA-C blank antigens on human blood lymphocytes." J.Immunol.145. 3714-3718 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamamoto, J., Hiraiwa, M., Hayashi, H., Tanabe, M., Kano, K., and Takiguchi, M.: "Two amino acid substitutions at residues 63 and 67 between HLA-B51 and HLA-Bw52 from multiple epitopes recognized by allogeneic T cells." Immunogenetics. 1991.33. 286-289 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura, M., Sakai, K., Akaza, T., Mitomi, Y., Nieda, M., Minami, M., and Juji, T.: "Anti-idiotypic antibody to T cell receptor in multiply transfused patients may play a role in resistance to graft-versus-host disease." Transfusion. 32. 719-728 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishimura, M., Sakai, K., Akaza, T., Mitomi, Y., Nieda, M., Minami, M., and Juji, T.: "Establishment of human minor histocompatibility antigen-specific cytotoxic T cell clones restricted by HLA-DR9." Transplantaion. 55. 1181-1186 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] M.Tanabe,M.Minami,K.Kano,M.Takiguchi: "The allorecognition of Hー2K^bーspecific CD4ーCD8ーT cell hybridomas is influenced by the substitution at residue 256 of MHC class I molecules" J.Immunology. 145. 1968-1973 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Nieda,T.Juji,S.Imao,M.Takamizawa,M.Minami: "Characterization of effector suppressor T cells in human mixed lymphocyte reaction"

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Nieda,T.Juji,S.Imao,M.Minami: "Specific killing of human CD4 T cell clones by CD8 cytotoxic T cell line"

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Hayashi,T.Ooba,S.Nakayama,M.Sekimata,K.Kano,M.Takiguchi: "Allospecificities between HLAーBw53 and HLAーB35 are generated by substitution of the residues associated with HLAーBw4/Bw6 public epitopes" Immunogenetics. 32. 195-199 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Sekimata,M.Hiraiwa,M.Andrien,E.Dupont,S.Karaki,J.Yamamoto,K.Kano,M.Takiguchi: "Allodeterminants and evolution of a novel HLAーB5 CREG antigen,HLAーB SNA" J.Immunollogy. 144. 3228-3233 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] J.Yamamoto,A.Kariyone,N.Akiyama,K.Kano,M.Takiguchi: "Presentation of human histocompatibility antigens by HLAーB35 and HLAーB38 molecules" Proc.Natl.Acad.Sci.USA. 87. 2583-2587 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yamamoto,J.,Tanabe,Minami,M.,Kano,K.,Takiguchi,M.: "Allo-class I-reactive CD4^-8^- T cell hybridomas recognize the conformational change of class I molecules resulting from the exchange of the α3 domain." Eur.J.Immunol.19. 1861-1865 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Takamizawa,M.,Juji,T.,Fujii,T.,Nieda,M.,Minami,M.: "Functional characterization of CD2^-3^+4^-8^-,TCRαβ^+T cell clones established from human peripheral lymphocytes."

    • Related Report
      1989 Annual Research Report
  • [Publications] Takiguchi,M.,Nishimura,I.,Hayashi,H.,Karaki,S.,Kariyone,A.,Takiguchi,M.: "The structure and expression of gen encoding serologically undetected HLA-Clocus antigens." J.Immunol.143. 1372-1378 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sekimata,M.,Hayashi,H.,Nakayama,S.,Kano,K.,Takiguchi,M.: "Serological demonstration of HLA-Bw4/Bw6 on hybrid molecules between HLA-B35 and HLA-B51." Immunogenetics. 30. 229-231 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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