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Immunosuppressive Therapy and Preservation in Liver Transplantation

Research Project

Project/Area Number 01480311
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionNagoya University

Principal Investigator

TAKAGI Hiroshi  Nagoya Univ. School of Med., Professor, 医学部, 教授 (70154755)

Co-Investigator(Kenkyū-buntansha) TOMITA Tadao  Nagoya Univ. School of Med., Professor, 医学部, 教授 (50078763)
HABA Toshihito  Nagoya Univ. School of Med., Assistant Professor, 医学部, 助手 (30228522)
HAYASHI Shuji  Nagoya Univ. School of Med., Assistant Professor, 医学部, 助手 (30218573)
HARADA Akio  Nagoya Univ. School of Med., Assistant Professor, 医学部, 助手 (50198909)
NONAMI Toshiaki  Nagoya Univ. School of Med., Assistant Professor, 医学部, 助手 (80189422)
佐久間 貞行  名古屋大学, 医学部, 教授 (90079963)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1990: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsLiver preservation / Endothelial cell / Factor VIII related antigen / FK506 / UW-solution / ^<31>P-NMR / NMR / 肝類洞内皮細胞 / 血液凝固関連因子Factor VIII / 部分肝移植 / 血行動態 / 類洞内皮細胞 / 血液凝固関連因子factor VIII
Research Abstract

I. Liver Preservation :
1. ^<31>P-NMR Study of Phospholipid Metabolites in Hypothermic-Preserved Liver.
Liver was perfused by Euro-Collins- or UW-solution, quickly resected and preserved at 4'C. By the ^<31>P-NMR study, it was clearly demonstrated that alpha-glycerophosphate (alpha-GP) increased in livers preserved in both solutions, but much pronounced in EC-solution. Thus, alpha-GP may reflect the extent of phospholipid degradation and correlate with organ viability.
2. Microvascular Changes of the Liver Preserved in UW-solution.
Livers preserved in UW solution or in Euro-Collins solution were examined with an immunohistochemical method using anti-Factor VIII related antigen (FRA). In EC group, endothelial cell damages was severe and FRA expression on the endothelium was diminished. Furthermore, sinusoid in zone III was filled with FRA-positive contents. In UW group, these changes were mild and only a few FRA-positive contents were recognized. Thus, UW solution prevents endothelial cell damage and microcirculatory injury in zone III during preservation period.
II. Immunosuppressant, FK506 :
1. Pathological and Immunohistochemical Examination in the Rat Treated with FK506.
Rats were administered FK506 for 4 days, and sacrificed on day 2, 5, 7, 9, and 11. Liver and pancreas were resected, fixed in PLP and immunostained using anti-FRA MoAb. Endothelium of sinusoid was not damaged and the levels of GOT and GPT showed S little increase. However, it was clearly demonstratedin UW group that endothelium of the vessels in pancreas was detached from basement membrane and lost its expression of FRA, and that serum amylase level markedly increased during FK506 administration. These change were reversible by the cessation of the FK506 administration. Thus, FK506 has a toxicity on vascular endothelium of pancreas and that has a possibility to cause pancreatitis.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] S.Hayashi: "How is the thymus affected by alteration in immunologic environment" Transplantation Proceedings. 21. 222-223 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Haba: "Microvascular changes of the liver preserved in UWーsolution ーー Pathological and immunohistochemical examination ーー" Cryobiology.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Haba: "Pathological and immunohistochemical examination in the rat treated with FK506" Transplantation Proceedings.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 蜂須賀 丈博: "新しいviability assayとしての保存肝リン脂質代謝産物の検討 ^<31>PーNMRを用いて" 日本移植学会雑誌. 25. 160-160 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shuji, Hayashi, et al.: "How is the thymus affected by alteration in immunologic environment" Transplantation Proceedings. Vol. 21. 222-223 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshihito, Haba, et al.: "Microvascular changes of the liver preserved in UW-solution. -- Pathological and immunohistochemical examination --" Cryobiology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshihito, Haba, et al.: "Pathological and immunohistochemical examination in the rat treated with FK506" Transplantation Proceedings.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takehiro, Hachisuka, et al.: " ^<31>P Nuclear magnetic resonance study of phospholipid metabolites in hypothermic-preservation" Transplantation Proceedings. Vol. 22. 485-487 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Hachisuka: " ^<31>P Nuclear magnetic resonance study of phospholipid metabolites in hypothermic preserved liver" Transplantation Proceedings. 22. 485-487 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 幅 俊人: "UW Solutionを用いてのラット肝保存の検討:肝保存における血管内皮細胞の意義" 日本外科学会雑誌. 90. 176-176 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 幅 俊人: "FK506投与時における血管内皮障害の検討" 日本移植学会雑誌. 25. 360-360 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 平 昇: "部分肝移植の現況と将来の展望ー1Donor,2Recipientの実験的研究ー" 日本消化器外科学会雑誌. 24. 344-344 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] N.Taira: "Partial liver transplantation in dogs:Preserviing the IVC of the recipient and use of a heparinized catheter(Anthron)for portal vein bypass." Transplantation Proceedings. 21(1). 2362-2362 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 林衆治: "ラット拒絶肝におけるNK細胞の意義" 日本消化器外科学会雑誌. 23. 344-344 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 幅俊人: "UW Solutionを用いてのラット肝保存の検討-組織所見及び組織ヌクレオチドの比較検討-" 日本消化器外科学会雑誌. 23. 341-341 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 蜂須賀丈博: "^<31>P NMR spectroscopyを用いた温阻血肝のリン脂質代謝産物の検討" 日本消化器外科学会雑誌. 23. 338-338 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1990-04-01   Modified: 2016-04-21  

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