| Project/Area Number |
01480333
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Medical College |
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Principal Investigator |
KIMURA Kouzaburo Tokyo Medical College, School of Medicine, Professor, 医学部, 教授 (90074509)
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| Co-Investigator(Kenkyū-buntansha) |
TANI Chiaki Tokyo Medical College, School of Medicine, Assistant professor, 医学部, 講師 (60147189)
TSURUI Shigeru Tokyo Medical College, school of Medicine, Fellow, 医学部, 助手 (30217385)
EIRAKU Hitoshi Tokyo Medical College, School of Medicine, Fellow, 医学部, 助手 (60203618)
KATOH Kohitiroh Tokyo Medical College, School of Medicine, Fellow, 医学部, 助手 (50201421)
NAKAJIMA Atsushi Tokyo Medical College, School of Medicine, Fellow, 医学部, 助手 (10211418)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥5,400,000 (Direct Cost: ¥5,400,000)
Fiscal Year 1990: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1989: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Colorectal Cancer / Irradiation therapy / Electron Irradiation / Nuclear DNA Content / Nuclear Protein Content / Immunostimulative Agents / Immunosuppressive Agents / ACNU [1-(4-amino-2-methlpyrimidin-5-y1)-methl-3-(2-chloroethy1)-3-nitrosourea-hydrochroride] / 大腸癌 / 直腸癌 / 核蛋白質 / 動物実験 |
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| Research Abstract |
Colon carcinoma cell line colon 26 was transplanted to mouse, and electron irradiation, ACNU and their combined therapy were performed for analysis of their effects. Tumor growing inhibition effect and survival rate of the groups with ACNU 20mg/kg and electron 3Gy were not statistical significantiy increased for the control group. These effects of the treated groups with ACNU 40mg/kg, electron 9Gy and ACNU 20mg/kg+electron 3Gy were statistical significant increased. DNA analysis suggested that these effects originated on G2 phase block. The results of histopathological analysis showed that the effect of ACNU 20mg/kg+electron 3Gy were similar with electron 9Gy. These results suggested additional ACNU made the electron dosage reduced for gaining the similar effect.
Immunostimulative and immunosuppressive agents were analyzed the effects for tumor bearing hosts. Cyclosporin A and Predonisolone as immunosuppressive agents were used. And beta-Glucan as anti tumor polysaccharide and OK-432 as Streptococcal preparation were used for immunostimulating agents. Survival rates showed that immunostimulating agents were statistical significantly increased. Immunosuppressive agents made the survival rate decreased, but not significantly. Two color analysis with flow cytometry using monoclonal antibodies Lyt 2 and L3T4 were performed for the study of immunological change by these agents. The results showed the tumor bearing mice had cells which could not find on normal mice. These cells were L3T4 negative and Lyt 2 weak positive. And these cell were increased with immunostimulating agents treatment. With dot plot analysis with Lyt 1, the same results were found
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