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Electrophysiologic and Histopathologic Evaluation of the Transplanted Heart

Research Project

Project/Area Number 01480346
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionTokyo Women's medical college

Principal Investigator

KOYANAGI Hitoshi  Tokyo Women's Medical College. Dept. of Cardiovascular Surgery. Professor., 医学部, 教授 (90138884)

Co-Investigator(Kenkyū-buntansha) NONOYAMA Masaki  Tokyo Women's Meddical College. Dept. of Cardiovascular Surgery. Assistant., 医学部, 助手 (40201701)
KITAMURA Masaya  Tokyo Women's Meddical College. Dept. of Cardiovascular Surgery. Assistant., 医学部, 助手 (90183300)
HACHIDA Mitsuhiro  Tokyo Women's Meddical College. Dept. of Cardiovascular Surgery. Assistant., 医学部, 助手 (00167587)
ENDO Masahiro  Tokyo Women's Meddical College. Dept. of Cardiovascular Surgery. Professor., 医学部, 教授 (20075302)
HASHIMOTO Akimasa  Tokyo Women's Meddical College. Dept. of Cardiovascular Surgery. Professor., 医学部, 教授 (90075185)
平田 欽也  東京女子医科大学, 医学部, 助手
江石 清行  東京女子医科大学, 医学部, 助手 (20167290)
榊原 尚豪  東京女子医科大学, 循環器外科学, 助手 (70119957)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥4,900,000 (Direct Cost: ¥4,900,000)
KeywordsHeart Transplantation / Rejection / Electrophysiologic Study / refractory Period / 食道ペ-シング / 電気生理学的方法 / 心筋拒絶反応 / 肺再潅流障害 / 免疫抑制療法 / 多臓器保存法 / UCLA溶液 / 急性拒絶反応 / 電気生理学的検査 / 病理組織学的検査 / 刺激伝導系
Research Abstract

Electrophysiologic and Histopathologic Evaluation of the Transplanted Heart
MATERIALS AND METHODS
Lewis (LEW/Crj) were donors of isografts or Lewis (LEW/Crj ; n=6) or Wistar King (WKAH/HKm ; n=12) recipients. Intra-abdominal heart transplantation (Tx) was performed by the method of Ono and Lindsey in adult male inbred rats (250 to 300g).
The hook-tipped bipolar endocardial leads were set up in the right atrial and right ventricular walls. ERP-AVCS was asseeed by programed extrastimulus method (basic cycle length of 150 msec) in early (2nd day), medium (7-9th day) and late (11-14th day) post-Tx periods.
Specimens of the conduction system and vetricular septum were examined liistopathologically at the end of final ERP study. A control group of isogenic Tx (between LEW/Crj rats : n=6) had no immunosuppressant. The allogenic Tx group (LEW/Cri WKAH/HK m ; n=12) was treated with cyclosporine (lOmg/kg/day) until sacrificed and six rats of this group were given prednisolone (I. 0 mg/kg/day) orally … More on the second postoperative week.
RESULTS
Control group showed no change in both ERP-AVCS and histopathologic findings. Individual values of ERP-AVCS obtained in days seven to nine were elevated 20% or higher than those of day two in all cases of allogenic Tx group. This change was statistically significant ; mean <plus-minus> SD were 84 <plus-minus> 6 msec on day two to 108 <plus-minus> 10 msec on day 7-9(p<0.0 1). Of these, six hearts were examined after medium ERP study, showing mononuclear cell infiltration without myocyte necrosis especially in the conduction system. ERP-AVCS of the remaining six rats on day 11-14 was recovered to initial values obtained on day two within 10% range. Their specimens showed less predominant cell infiltration. Tlie ERP-AVCSs of one allograft (Oo. 9) are represented. The ERP-AVCSof this heart was prolonged from 84 msec on post-Tx day two to 114 msec on day 7 and recovered to 88 msec on postoperative d
CONCLUSIONS
These results suggest that the prolongation of ERP-AVCS could be a useful paramenter for diagnosing mild acute rejection in the prenecrotic stage under immunosuppressive treatment following heart transplantation. This offers that the electrophysiologic monitoring may be suitable for use during the first four to six weeks after heart transplantation, while the appropriate epicardial transcutaneous electrodes are in place. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Masaya Kitamura,et al.: "Electrophysiologic and Histopathologic Evaluation of the Transplanted Rat Heart." Transplantation Proceedings. 21(1). 2544-2546 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 北村 昌也,他: "急性拒絶反応のラット移植心の電気生理学的変化 病理組織学的所見との対応" 今日の移植. 2(6). 456-460 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 八田 光弘,他: "Multiple Organ Procurementーその手枝、保存方法、Viablityー" 日本外科学会雑誌. 92(2). 214-221 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 北村 昌也,他: "右供大静脈洞切開・吻合法によるイヌ同所性心臓移植ー洞結節及び右供組織全体の温存ー" 日本胸部外科学会雑誌. 39(2). 37(169)-41(173) (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 八田 光弘,他: "心肺同時移植後の心筋拒絶反応についての組織学的考察(I)・(II)" 日本胸部外科学会雑誌. 37(11). 6(2250)-17(2261 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 八田 光弘,小柳 仁,他: "心臓移植・肺移植ー技術評価と生命倫理に関する総括レポ-トー" 金芳堂, 189 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Koyanagi,et al.: "Artificial Heart 3" Springer-Verlag, 351 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masaya Kitamura, et al.: "Electrophysiologic and Histopathologic Evaluation the Transplanted Rat Heart." Transplantation proceedings. 21, No 1. 2544-2546 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, et al.: "A New Multiple Organ Preservation-Assessment of Viability After the Heart and Lung Preservation U Sing Ucla Formula." Journal of Japan Surgical Society. 92, No 2. 214-220 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masaya Kitamura, et al.: "Orthotopic Heart Transplantation with Sinus Venosus of Right Atrium in Dogs -Preservation of Sinus Node and Oridinal Right Atrium-." The Journal of the Japanese Association for Thoracic Surgery. 39, No 2. 37(169)-41(173) (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masaya Kitamura, et al.: "Selection of Candidates for The Ventricular Assist Device as a Bridge to Heart Transplantation." The Journal of the Japanese Association for Thoracic Surgery. 37, No 7. 69(1341)-74(1346) (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, et al.: "Assessment of Cardiac Rejection Following Heart and Lung Transplantation (I)." The Journal of the Japanese Association for Thoracic Surgery. 37, No 11. 6(2250)-11(2255) (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, et al.: "Assessment of Cardiac Rejection Following Heart and Lung Transplantation (II)." The Journal of the Japanese Association for Thoracic Surgery. 37, No 11. 12(2256)-16(2260) (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, et al.: "Lung function after prolonged lung preservation." The journal of Thotacic and Cardiovascular Surgery. 97, No 6. 911-919 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, Hitoshi Koyanagi.: "Increase of Surfactant and Protein Content in the Alveolar space After Lung Preservation." The Journal of the Japanese Association for Thoracic Surgery. 38, No 9. 30(1424)-34(1428) (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, et al.: "Significant Immunosuppressive Effect of Ganglioside GM_3 in Organ Transplantation." Transplantation Proceedings. 22, No 4. 1663-1665 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hachida, Hitoshi Koyanagi.: "Assessment of Tumor Growth after Heterotopic Heart and Lung transplant in rat" Japanese Journal of Transplantation. 25, No 1. 69-73 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mitsuhiro Hchida, et al.: "New ERA ; Heart Transplantation in Japan -Problem and Solution-." Journal of Japan Surgical Society. 91, NO 9. 1403-1405 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 八田 光弘,小柳 仁: "心肺同時移植後の問題点と展望" 「癌・免疫・栄養」誌. 5(3). 14-23 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 八田 光弘,野々山 真樹,小柳 仁,他: "心臓移植の拒絶反応の診断" 今日の移植. 4(3). 281-288 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 北村 昌也,今村 伸一郎,他: "右房大静脈洞切開・吻合法によるイヌ同所性心臓移植ー洞結節及び右房組織全体の保存方法ー" 日本胸部外科学会雑誌. 39(2). 37(169)-41(173) (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 北村 昌也,広田 潤,他: "イヌ同所性心臓移植における傍食道左房吻合法ー経食道心房ペ-シングによる電気生理学的検査の可能性ー" 胸部外科. 44(4). 299-302 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 八田 光弘,小柳 仁,他: "multiple organ procurementーその手技、保存方法、Viability." 日本外科学会雑誌. 92(2). 214-221 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 八田 光弘,小笠原 定雅,他: "心不全体系の中の心蔵移植" ハ-トナ-シング. 4(1). 79-82 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 八田 光弘,小柳 仁,他: "心蔵移植・肺移植ー技術評価と生命倫理に関する総括レポ-トー" 金芳堂, 189 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] H.Koyanagi,et al.: "Artificial Heart 3" Springer-Verlag, 351 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 八田 光弘,小柳 仁他: "Multiple organ procurementーその手技,保存方法,Viability" 日本外科学会誌. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 八田 光弘,小柳 仁他: "脳死下臓器移植としての心,心肺移植の問題点と展望" 日本外科学会誌. 91. 1403-1405 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 小柳 仁,八田光弘他: "拡張型心筋症,ー心臓移植の適応基準" 最新医学社. 45. 1931-1937 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] M.Hachida et al.: "Significant Immuno suppressive Effect of Ganglioside GM_3 in Ozgan Transplantation" Transplantation Proceedings. 22. 1663-1665 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 八田 光弘,小柳 仁: "肺保存後の虚血ー再潅流障害の肺胞系変化に関する研究" 日本胸部外科学会雑誌. 38. 1424-1428 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 八田 光弘,小柳 仁: "心肺同時移植後の癌細胞発育に関する実験的検討" 移植. 25. 69-73 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masaya Kitamura,et al.: "Electrophysiologic assessment of the transplanted canine heart" Journal of Heart Transplantation. 7. 213-222 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 北村昌也: "イヌ異所性移植心内電気生理学的及び病理組織学的検討" 日本胸部外科学会雑誌. 36. 961-968 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Masaya Kitamura,et al.: "Electrophysiologic and Histopathologic Evaluation of the transplanted Rat Heart" Transplantation Proceeding. 21. 2544-2546 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 北村昌也,他: "急性拒絶反応時のラット移植心の電気生理学的変化" 今日の移植. 2. 456-460 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 北村昌也,他: "正中切開・交叉循環下のイヌ同所性心臓移植ー大静脈洞切開・洞結節側位による右房吻合法ー" 日本移植学会雑誌. 25. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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