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Transplantation of genetically modified cultured neurons into the brain with Parkinson's disease.

Research Project

Project/Area Number 01480361
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Cerebral neurosurgery
Research InstitutionKeio University

Principal Investigator

TOYA Shigeo  Keio Univ., School of Medicine, Professor, 医学部, 教授 (40051205)

Co-Investigator(Kenkyū-buntansha) UEMURA Keiichi  Keio Univ., School of Medicine, Professor, 医学部, 教授 (90049792)
ASADA Hideo  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (10184144)
YOSHIDA Kazunari  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (70166940)
TAKAYAMA Hideichi  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (50171559)
OTANI Mitsuhiro  Keio Univ., School of Medicine, Instructor, 医学部, 助手 (80051605)
高坂 新一  国立精神神経センター, 代謝研究部, 部長 (50112686)
高野 利也  慶應義塾大学, 医学部, 教授 (60051364)
中野 幸照  慶應義塾大学, 医学部, 助手 (70172377)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1991: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1990: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsParkinson's disease / Transplantation / Cultured neuron / Nerve growth factor / Neuroblastoma / Retinoic acid / Vascularization / Rejection / NGF / 遺伝子導入細胞 / 脳内移植
Research Abstract

1. The reconstruction, the origin, and the blood-brain-barrier (BBB) of the blood vessels of mouse cortical tissue grafted into the third ventricle of rat brain were investigated. The following are the results of the study. 1) Some of the blood vessels in the graft were derived from the donor mouse tissue. 2) The host blood was supplied to the blood vessels derived from the donor. 3) BBB is existent iii the graft from 5th day after transplantation, but injured at the 14th post-operative day presumably by the immunological rejection mechanism.
2. The immunological rejection in the xenograft was sufficiently suppressed by FK-506, the product of Streptomyces tsukubaensis.
3. Human neuroblastoma cells (SH-SY5Y) were found to differentiate to mature neurons following retinoic acid treatment in vitro. The differentiated SH-SY5Y cells were grafted into the brain of Wistar albino rats, Balb/c mice, and nude inice. The grafted differentiated SH-SY5Y cells did not survive because of the immunological rejection and other reasons. However, the intravetriclar administration of NGF significantly promoted the survival of the graft. These results suggest that the cultured neurous derived from human Neuroblastoma cells could be a source of graft for Parkinson's disease.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 浅田 英穂,高山 秀一,吉田 一成,中野 幸照,陳 宗孚,大谷 光弘,戸谷 重雄: "ヒト神経芽細胞腫培養神経細胞のラットへの脳内移植" 神経組織の再生・成長・移植. 1. 27-28 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yukiteru Nakano,Kohtaro Take,Shigeo Toya,Yasuzo Tsukada,Said Ghandour,Shinichi Kohsaka: "Mosaic reconstruction of blood vessels in mouse neocortical tissre transplanted into the third ventricle of brain." Brain Research. 496. 336-340 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 浅田 英穂,高山 秀一,中野 幸照,陳 宗孚,杜子威,河瀬 文武,大谷 光弘,戸谷 重雄: "ヒト神経芽細胞腫培養細胞の脳内移植における免疫学的検討および脳室内NGF投与の効果" 神経組織の再生・成長・移植. 2. 112-113 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 浅田 英穂,高山 秀一,中野 幸照,陳 宗孚,杜子威,大谷 光弘,戸谷 重雄: "ヒト神経芽細胞腫培養細胞のラットおよびマウス脳内移植における免疫学的検討" Neuroimmunological Research. 3. 32-37 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 浅田 英穂,白尾 智明,戸田 正博,戸谷 重雄,植村 慶一: "培養ヒト神経芽細胞腫SY5Y細胞のレチノ-ル酸による分化におけるニュ-ロフィラメントおよびドレブリンの変化" 神経組織の再生・成長・移植. 3. 92-93 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中野 幸照: "異種間脳内移植における組織内血管構築と血液脳関門に関する基礎的研究" 慶應医学. 68. 607-618 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Asada H. et al.: "Transplantation of cultured human neuroblastoma cells into rat brain" Neural Growth, Regeneration and Transplantation. 1. 27-28 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakano Y, et al.: "Mosaic reconstruction of blood vessels in mouse neocortical tissue transplanted into the third of rat brain." Brain Research. 496. 336-340 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Asada H, et al.: "Immunological study on transplatation of human neuroblastoma cultured cells into mouse brain : Effect of intraventricular administration of NGF." Neural Growth, Regeneration and Transplantation. 2. 112-113 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Asada H, et al.: "Immunological study on transplantation of human neuroblastoma cultured cells into mouse brain." Immunological Research. 3. 32-37 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Asada H. et al.: "Changes of neurofilament and drebrin during differentiation of human neuroblastoma cells by retinoic acid." Neural Growth, Regeneration and Transplantation. 3. 92-93 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nakano Y.: "The reconstruction of blood vessels and blood-brain barrier in the xenogeneic neural transplatation." Keio Igaku. 68. 607-618 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 中野 幸照: "異種間脳移植における組織内血管構築と血液脳関門に関する基礎的研究" 慶應医学. 68. 607-618 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 浅田 英穂 他: "培養ヒト神経芽細胞腫SY5Y細胞のレチノ-ル酸による分化におけるニュ-ロフィラメントおよびドレブリンの変化" 神経組織の再生・成長・移植. 3. 92-93 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 浅田 英穂: "ヒト神経芽細胞腫培養細胞の脳内移植における免疫学的検討および脳室内NGF投与の効果" 神経組織の成長,再生,移植. 2. 112-113 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 浅田 英穂: "ヒト神経芽細胞腫培養細胞のラットおよびマウス脳内移植における免疫学的検討" 神経免疫研究. 3. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nakano: "Xenogeneic Neural Transplantation;Role of Vasculature and MHC Antigen in Immunological Rejection" Stereotactic and Functional Neurosurgery. 54. 358-363 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高山 秀一: "FK506の異種間脳移植におけるグラフトの生着に及ぼす効果" 神経組織の成長,再生,移植. 2. 66-67 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 浅田英穂: "ヒト神経芽細胞腫培養神経細胞のラットへの脳内移植" 神経組織の成長・再生・移植. 1. 27-28 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Nakano: "Mosaic reconstruction of blood vessels in mouse neocortical tissue transplanted into the third ventricle of rat brain." Brain Research. 496. 336-340 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 井上洋: "末梢神経組織の移植による中枢神経系の再生" Clinical Neuroscience. 7. 416-419 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Nakano: "Xenogeneic neural transplantation:Role of vasculature and MHC antigen in Immunological rejection." Applied Neurophysiology. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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