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Protection of Spinal Cord Injury, Experimental and Clinical Study on Scopic Surgery.

Research Project

Project/Area Number 01480369
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionThe University of Tokushima

Principal Investigator

IKATA Takaaki  The University of Tokushima School of Medicine Professors, 医学部, 教授 (80108860)

Co-Investigator(Kenkyū-buntansha) MITSUHASHI Tadashi  The University of Tokushima School of Medicine Research Associates, 医学部, 助手 (40229745)
TAKAI Hiroaki  The University of Tokushima School of Medicine Professors, 医学部・附属病院, 講師 (40197041)
MURASE Masaaki  The University of Tokushima School of Medicine Assistant Professors, 医学部・附属病院, 講師 (00182121)
FUKAZAWA Kenji  The University of Tokushima Faculty of Pharmaceutical Sciences. Associate Profes, 薬学部, 助教授 (90035551)
OKA Motoo  The University of Tokushima School of Medicine Professors, 医学部, 教授 (60028298)
森本 訓明  徳島大学, 医学部附属病院(整形外科学), 助手 (80210186)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1990: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1989: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsSpinal cord injury / Thromboxane B_2 / 6-keto-prostaglandin F, alpha / Vascular damage / Leukotriene C_4 / Ascorbic Acid / alpha-tocopherol / Vitamin E / αートロクエロ-ル / プロスタグランディン / VitE欠乏ラット / TBARS / トロンボキサンA_2 / 選択的トロンボキサンA_2合成酵素阻害剤
Research Abstract

Experimental Spinal Cord Injury : 1) In a rat spinal cord compression injury model, eicosanoids production was determined by radioimmunoassay. The increased TXB_2 production was inhibited more than 80% by OKY-046, but the degree of vascular damage was reduced by only 40%. In a guinea pig spinal cord compression model, TXB_2 production was dependent on the degree of compression strength, while LTC_4 production was not affected by the degree of compression injury. The LTC_4 production confirmed in the injured spinal cords suggested its relationship to secondary disorder in the spinal cord injury. 2) ODS rats were used for experiments. Studies were made on the influence of Vitamin C on the effect of compression injury of the spinal cord associated with ischemia in rats. We investigated the level of ascorbic acid and alpha-Tocopherol in the serum and spinal cord tissue, and the level of thiobarbituric acid (TBA)-reactive substances in the spinal cord. Level of ascorbic acid and alpha-Tocop … More herol in the serum and spinal cord are decresed in the Vitamin C-deficient group and Vitamin C-supplemented group. The motor disturbance induced by spinal cord injury was greatly reduced by Vitamin C supplementation. Pathological examination of the spinal cord showed less damage, such as bleeding, in the Vitamin C-supplemented group. In the spinal cord injury, Vitamin C and Vitamin E may prevent tissue damage by inhibiting lipid peroxidation in the separate phases. Experiment of microscopic surgery : 1) We researched on osteoinductive activity by histological study, using the models of pseudoarhrotic type spondylolysis in the 5th lumbar spine of Wistar rats. Methods of restration were to use transplantation of auto cancellous bone, auto cancellous bone+fibrin glue ; F group and F group+Bone Morphogenetic Protein ; BMP group. Though perfect bone fusion was not obtained through all methods, bone formation through BMP group was superior to other groups. Consiouently it is suggested that there is a possibility of clinical application by using transplantation of BMP group with microscopic method. 2) The efficacy of anterior decompression by the trans-vertebral approach was evaluated in the following studies. To determine whether the reherniation and degeneration of segment motion after an anterior decompression by the trans-vertebral approach, the posterior part of the cervical disc of bipedal rat was extirpated with the same procedure. Progressing disc degeneration was noted at the operated level compared to the adjacent disc. The experimental results, included effective decompression and no serious coplication are encouraging. Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (54 results)

All Other

All Publications (54 results)

  • [Publications] Takaaki Ikata et.al: "Clinical Considerrations and Bochemical Basis of Prognosis of Cervical Spinal Cord Injury." Spine. 14. 1096-1101 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 井形 高明、森本 訓明,藤内 武春: "脊髄損傷とプロスタブランジン" 現代医療. 21. 2776 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 田岡 祐二,井形 高明,岩佐 賢一,福沢 健治: "実験的脊髄損傷に対するVitaminE.欠乏の影響" 中部日本整形外科災害外科学会雑誌. 32. 138-140 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 藤内 武春、井形 高明、森本 訓明、正木 国弘: "損傷脊髄におけるTXA_2産生増加と血管透過性" 日本パラプレジア医学会雑誌. 2. 120-121 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 森本 訓明,井形 高明,正木 国弘,藤内 武春: "OKYー046のラット脊髄損傷におけるTXA_2産生と血管透過性に対する効果" 日本パラプレジア医学会雑誌. 2. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 森本 訓明,井形 高明,藤内 武春: "選択的TXA_2合成酸素阻害剤OKYー046のラット脊髄損傷におけるトロンボキサンB_2産生と血管損傷に対する効果" 日本形形外科学会雑誌. 64. 82-88 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuuji Taoka,Takaaki Ikata,Kenji Fukuzawa: "Influence of Dietory VitaminE Deficiency on Compression Injury of Rat Spinal Cord" J.Nutr.Sci.Vitaminol. 36. 217-226 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 三橋 雅,井形 高明,村瀬 正昭,森本 訓明,藤内 武春: "損傷脊髄におけるロイコトリエンC_4産生増加" 日本整形外科学会雑誌. 64. 1106- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 三橋 雅,井形 高明,村瀬 正昭,森本 訓明,藤内 武春: "損傷脊髄におけるロイコトリエンC_4産生増加" 日本パラプレジア医学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 加藤 大輔,井形 高明,村瀬 正昭,田岡 祐二,福沢 健治: "実験的脊髄損傷におけるビタミンCとビタミンEの関連について" 日本整形外科学会雑誌. 64. 1107- (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 加藤 大輔,井形 高明,村瀬 正昭,田岡 祐二,福沢 健治: "実験的脊髄損傷におけるビタミンCとビタミンEの関連について" 日本パラプレジア医学会雑誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 加藤 大輔,井形 高明,田岡 祐二,福沢 健治: "ODSラットのE量におよぼすC欠乏の影響と脊趨損傷時の量的変化" ビタミンE研究の進歩.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 福沢 健治: "ビタミンEとプロスタグラジン" 現代医療21. 21. 1251- (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 福沢 健治,高石 喜久: "抗酸化剤" 活性酸素、フリ-ラジカル. 1. 55-70 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomohito Fujii,Yasushi Hiramoto,Junji Terao,Kenji Fukuzawa: "SiteーSpecific Mechanisms of Initiation by Chelated Iron and Inhibition by αーTocopherol of Lipid PeroxideーDependent Lipid Peroxidation in Charged Micelles." Archives of Biochemisitry and Biophysics. 284. 120-126 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hozumi Iwasa,Toshihiro Aono,Kenji Fukuzawa: "Protective effect of Vitamin E on Fetal Distress Induced by Ischemia of the Uteroplacental system in Pregnant Rats." Free Radical Biology & Medicine. 8. 393-400 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kenji Fukuzawa,Yasuhiro Kurotori,Akira Tokumura,Hiroaki Tsukatani: "Vitamin E Deficiency Increases the Synthesis of PlateletーActivating Factor(PAF)in Rat Polymorphonuclear Leucocytes." Lipids. 24. 236-239 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Atsushi Nakanishi,Masanori Yoshizumi,Shuichi Hamano,Kyoji Morita,Motoo Oka: "Myosin lightーchain kinase inhibitor,1ー(5ーchlorーnaphthaleneー1ーsulfonyl)ー1Hーhaxahydroー1,4ーdiazepine (MLー9),inhibits catecholamine secretion from adrenal chromaffin cells by inhibiting Ca^<2+>uptake into the cells" Biochemical Pharmacology. 38. 2615-2619 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Manabu Yoshikawa,Haruhiko Saito,Toshiaki Sano,Takeshi Ohuchi,Yasuko Ishimura,Kyoji Morita,Shiro Saito,: "Localization and release of immunoreactive vasoactive intestinal polypeptide in bovine adrenal medulla." Neuroscience Letters. 111. 75-79 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Atsushi Nakanishi,Masanori Yoshizumi,Kyoji Morita,Yoshihiro Murakumo,Hitoshi Houchi,Motoo Oka: "Palytoxin,a potent stimulator of catecholamine release from cultured bovine adrenal chromaffin cells" Neuroscience Letters. 121. 163-165 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 田岡 祐二,井形 高明,岩佐 賢一,加藤 大輔,福沢 健治: "ビタミンE研究の進歩I 食餌性ビタミンEによるラット脊髄圧迫損傷の防御作用" エイド出版, (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takaaki Ikata, et al.: "Clinical Considerations and Biochemical Basis of Prognosis of Cervical Spinal Cord Injury." Spine. Vol. 14. 1096-1101 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takaaki Ikata, et al.: "Prostaglandin in. Spinal Cord Injury" Gendaiiryou. Vol. 21. 2776-1989

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuuji Taoka, et al.: "Effect of OKY-046 on production of TXB_2 and vascular Permeability." The Central Japan Journal of Orthopaedic Surgery & Traumatology. Vol. 32. 138-140 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takeharu Tounai, et al.: "Increased Production of TXA_2 and Vascular Permeability in Spinal Cord Injury." The Journal of the Japan Medical Society of Paraplegia. Vol. 2. 120-121 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kuniaki Morimoto, et al.: "Effect of OKY-046 on Production of TXA_2 and Vascular Permeability in Spinal Cord Injury." The Journal of the Japan Medical Society of Paraplegia. Vol. 2. 122- (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kuniaki Morimoto, et al.: "Beneficial Effect of a Selective TXA Synthetase Inhibitor, OKY-046, both on Thromboxane B Production and Vascular Damage after Spinal Cord Injury in Rat Spinal Cord." The Journal of the Japanese Orthopaedic Association. Vol. 64 No. 1. 82-88 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuuji Taoka, et al.: "Influence of Dietary Vitamin E Deficiency on Compression Injury of Rat Spinal Cord." J. Nutr. Sci, Vitaminol. Vol. 36. 217-226 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Mitsuhashi, et al: "Increased Production of LTC_4 in Experimental Spinal Cord Injury" The Journal of the Japanese Orthopaedic Association. Vol. 64. 1106- (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Mitsuhashi, et al: "Increased Production of LTC_4 in Experimental Spinal Cord Injury." The Journal of the Japan Medical Society of Paraplegia.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Daisuke Katoh, et al.: "An Experimental Study of the Effect of Vitamin C and Vitamin E in Spinal Cord Injury." The Journal of the Japanese Orthopaedic Association. Vol. 64. 1107- (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Daisuke Katoh, et al.: "An Experimental Study of the Effect of Vitamin C and Vitamin E in Spinal Cord Injury." The Journal of the Japan Medical Society of Paraplegia.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Daisuke Katoh, et al.: "Influence of Dietary Vitamin C Deficiency on Compression Injury of ODS Rat Spinal Cord. -Correlation of Vitamin C and Vitamin E-" Progress in Study of Vitamin E.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kenji Fukuzawa: "Vitamin E and Prostaglandin" Gendaiiryou. Vol. 21. 1251- (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kenji Fukuzawa, et al.: "Antioxydants" Journal of Active Oxygens & Free Radicals. Vol. 1. 55-70 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomohito Fujii, et al.: "Site-Specific Mechanisms of Initiation by Chelated Iron and Inhibition by alpha-Tocopherol of Lipid Peroxide-Dependent Lipid Peroxidation in Charged Micelles." Archives of Biochemisitry and Biophysics. Vol. 284. 120-126 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hozumi Iwasa, et al.: "Protective effect of Vitamin E on Fetal Distress Induced by Ischemia of the Uteroplacental system in Pregnant Rats." Free Radical Biology & Medicine. Vol. 8. 393-400 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kenji Fukuzawa, et al.: "Vitamin E Deficiency Increases the Synthesis of Platelet-Activating Factor. (PAF) in Rat Polymorphonuclear Leucocytes." Lipid. Vol. 24. 236-239 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Atsushi Nakanishi, etal.: "Myosin light-chain kinase inhibitor, 1-(5-chlor-naphthalene-1-sulfonyl)-1H-Haxahydro-1, 4-diazepine (ML-9), inhibits cate-cholamine secretion from adrenal chromaffin cells by inhibiting Ca^<2+> uptake into the cells" Biochemical Pharmacology. Vol. 38. 2615-2619 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Manabu Yoshikawa, et al.: "Localization and release of immunoreactive vasoactive intestinal polypeptide in bovine adrenal medulla." Neuroscience Letters. Vol. 111. 75-79 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Atsushi Nakanishi, et al: "Palytoxin, a potent stimulato of catecholamine release from Cultured bovine adrenal chromaffin cells." Neuroscience Letters. Vol. 121. 163-165 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yuuju Taoka, et al.: "Preventive Effect of Vitamin E on compression Injury of Rat Spinal Cord." Progress in Study of Vitamin E.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 森本 訓明,井形 高明,藤内 武春: "選択的TXA_2合成酵素阻害剤OKYー046のラット脊髄損傷におけるトロンボキサンB_2産生と血管損傷に対する効果" 日本整形外科学会雑誌. 64. 82-88 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 福沢 健治,高石 喜久: "抗酸化剤" 活性酸素、フリ-ラジカル. 1. 55-70 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tomohito Fujii,Yasushi Hiramoto,Junji Terao,Kenji Fukuzawa: "SiteーSpecific Mechanisms of Initiation by Chelated Iron and Inhibition by αーTocopherol of Lipid PeroxideーDependent Lipid peroxidation in Charged Micelles." Archives of Biochemisitry and Biophysics. 284. 120-126 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hozumi Iwasa,Toshihiro Aono,Kenji Fukuzawa: "Protective effect of Vitamin E on Fetal Distress Induced by Ischemia of the Uteroplacental system in Pregnant Rats." Free Radical Biology & Medicine. 8. 393-400 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Manabu Yoshikawa,Haruhiko Saito,Toshiaki Sano,Takeshi Ohuchi,Yasuko Ishimura,Kyoji Morita,Shiro Saito Motoo Oka: "Localization and release of immunoreactive Vasoactive intestinal polypeptide in bovine adrenal medulla." Neuroscience Letters. 111. 75-79 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Atsushi Nakanishi,Masanori Yoshizumi,Kyoji Morita,Yoshihiro Murakumo,Hitoshi Houchi,Motoo Oka: "Palytoxin,a potent stimulator of catecholamine release from cultured bovine a drenal chromaffincells." Neuroscience Letters. 121. 163-165 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田岡祐二,井形高明,岩佐賢一,福沢健治: "実験的脊髄損傷に対するVitaminE欠乏の影響" 中部日本整形外科災害外科学会誌. 32. 138-140 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 井形高明,正木国弘,森本訓明,藤内武春,田岡祐二: "特集、脊髄損傷ー初期治療ー" 救急医学. 12. 1599-1605 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 井形高明,岩佐賢一,森本訓明,藤内武春,田岡祐二: "Clinical Consideration and Biochemical Basis of Prognosis of Cervical Spinal Cord Injury" Spine by J.B.Lippincott Co.14. 1096-1101 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 井形高明,森本訓明,藤内武春: "脊髄損傷とプロスタグランジン" 現代医療. 21. 2776 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 森本訓明,井形高明,藤内武春: "選択的TXA_2合成酵素阻害剤OKY-046のラット脊髄損傷におけるトロンボキサンB_2産生と血管損傷に対する効果" 日本整形外科学会雑誌. 64. 82-88 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 森本訓明,井形高明,村瀬正昭,加藤大輔,三橋雅,福島孝: "重錘圧迫法による実験的脊髄損傷の病態に関する生化学的検討" 日本パラプレジア医学会誌.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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