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Studies on the Pathogenesis of Trophoblastic Tumors by DNA Fingerprint and its Relationship With Growth Factor

Research Project

Project/Area Number 01480394
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionOsaka University Medical School

Principal Investigator

KOYAMA Masayasu (1990)  Osaka University, Medical School Department of Obstetrics and Gynecology, Clinical fellow, 医学部, 助手 (00183351)

東 千尋 (1989)  大阪大学, 医学部, 助手 (20151061)

Co-Investigator(Kenkyū-buntansha) OHASHI Kazutomo  Osaka University, Medical School Department of Obstetrics and Gynecology, Clinic, 医学部, 助手 (30203897)
SAJI Fumitaka  Osaka University, Medical School Department of Obstetrics and Gynecology, Assist, 医学部, 講師 (90093418)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1990: ¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1989: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsDNA fingerprint / Hydatidiform mole / Choriocarcinoma / Androgeners / Mitochondrial DNA / Macrophage colony-stimulating factor / ミニサテライトDNAプロ-ブ / サザンブロットハイブリダイゼ-ション / ノ-ザンブロットハイブリダイゼ-ション / MCSF / c-fms
Research Abstract

We have investigated the pathogenesis of trophoblastic tumors by analyzing restriction fragment length polymorphisms (RFLPs) using a minisatellite DNA probe (DNA fingerprint) to complete hydatidiform molecules, riocarcinoma, and hydrotropic abortus. The polymorphic fragments of the complete mole were all transmitted from the paternal DNA, while some of the polymorphic bands of paternal DNA were not observed in molar DNA (placenta 1989, 10, pp399). On the other hand, the molar mitochondrial DNA was inherited from maternal ovum (Gynecol Oncol 1991, 40, pp29). Our results verify that complete molecule results from the fertilization of an anuclear empty ovum with normal sperm at the molecular level. In the hydrotropic abortus, the polymorphic fragments of the DNA fingerprint could be traced back to its parent, indicating that the DNA fingerprint could be a useful tool for differential diagnosis between complete molecules and hydrotropic abortus (Am J Obstet Gynecol 1990, 163, pp634).
Intere … More stingly, in some cases of choriocarcinoma, the pattern of inheritance of RFLPs was the same as that in the complete molecule, Whereas in others all the polymorphic fragments in tumor tissues were identical to those in the host DNA (Cancer Res 1990, 50, pp488). These results suggest that the pathogenesis of choriocarcinoma varies, being completely different from that of the complete hydatidiform mole in some cases.
We also investigated the gene expression of the macrophage colony-stimulating factor (M-CSF) and its receptor, c-fms proto-oncogene, in human placenta and decidua in order to clarify the role of this growth factor in the proliferation of trophoblasts. M-CSF and c-fms mRNAs, 4.7kb and 3.9kb respectively, were detected by Northern blot hybridization in the early stage placenta and subsequently increased during pregnancy. These results indicate that M-CSF is deeply involved in the local proliferation and differentiation of trophoblasts and the auto- or paracrine system of this growth factor may play a key role of the growth of trophoblastic tumors (Am J Reprod Immunol 1990, 24, pp99). Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Fumitaka Saji: "Gene expression of macrophage colonyーstimulating factor (MーCSF) and its receptor(cーfms) in human placenta and decidua." Am.J.Reprod.Immunol.24. 99-104 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chihiro Azuma: "Steroid hormones induce macrophage colony stimulating factor (MCSF) and MCSF receptor mRNAs in the human endometrium" J. Molecular Endocrinology. 5. 103-108 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chihiro Azuma: "Application of gene amplification by polymerase chain reaction(PCR) to genetic analysis of molar mitochondrial DNA;the detection of anuclear empty ovum as the cause of complete mole." Gynecologic Oncology. 40. 29-33 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshikatsu Nobunaga: "Differential diagnosis between complete mole and hydropic abortus by deoxyribonucleic acid fingerprints" Am.J.Obstet.Gynec.163. 634-638 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chihiro Azuma: "Studies on the pathogenesis of choriocarcinoma by analysis of restriction fragment length polymorphisms" Cancer.Res.50. 488-491 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 信永 敏克: "分子生物学的手技を用いた胞状奇胎の発生病理の解析" 産婦人科の進歩. 41. 161-163 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 東 千尋: "産婦人科領域への分子生物学の応用 ーーバイオテクノロジ-を用いた 診断と治療ーー DNA指紋を用いた多胎の卵性診断" 産科と婦人科. 56. 2377-2382 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 佐治 文隆: "産婦人科領域への分子生物学の応用 ーーバイオテクノロジ-を用いた 診断と治療ーー 診断・治療への展望" 産科と婦人科. 56. 2361-2366 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masahiko Takemura: "Determination of fetal sex from maternal blood by DNA amplication" Medical Reproductive Immunology. 135-138 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chihiro Azuma: "RFLPs analysis of extranuclear mithochondrial DNA of complete mole by Using gene amplication method of PCR" Proceeding of the 4th Annual meeting of Japan Society for Basic Reproductive Immunology. 100-104 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumitaka Saji: "A new approach using DNA fingerprinting for the determination of androgenesis as a cause of hydatidiform mole" Placenta. 10. 399-406 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Chihiro Azuma: "Zygosity Determination of multiple pregnancy by DNA fingerprinting" Am.J.Obstet.Gynec.160. 734-736 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] C. Azuma, F. Saji, Y. Tokugawa, T. Kimura, T. Nobunaga, M. Takemura, T. Kameda, and O. Tanizawa.: "Application of gene amplification by polymerase chain reaction to genetic analysis of molar mitochondrial DNA : the detection of anuclear empty ovum as the cause of complete molecules." Gynecologic Oncology. 40. 29-33 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] F. Saji, C. Azuma, T. Kimura, M. Koyama, K. Ohashi, and O. Tanizawa.: "Gene expression of macrophage colony-stimulating factor and its receptor in human placenta and decidua." Am. J. Reproduc. Immunol.24. 99-104 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] C. Azuma, F. Saji, T. Kimura, Y. Tokugawa, M. Takemura, Y. Samejima, and O. Tanizawa.: "Steroid hormones induce macrophage colony-stimulating factor (MCSF) and MCSF receptor mRNAs in the human endometrium." J. Mole. Endocrinol. 5. 103-108 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] C. Azuma, S. Kamiura, T. Nobunaga, T. Negoro, F. Saji, and O. Tanizawa.: "Zygosity determination of multiple pregnancy by DNA fingerprinting." Am. J. Obstet. Gynec. 160. 734-736 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Fumitaka Saji: "Gene expression of macrophage colonyーstimulating factor (MーCSF) and its receptor(cーfms) in human placenta and decidua." Am.J.Reprod.Immunol.24. 99-104 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chihro Azuma: "Steroid hormones induce macrophage colony stimulating factor (MCSF) and MCSF receptor mRNAs in the human endometrium" Gynecologic Oncology. 5. 103-108 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chihiro Azuma: "Application of gene amplification by polymerase chain reaction(PCR) to genetic analysis of molar mitochondrial DNA;the detection of anuclear empty ovum as the cause od complete mole." Gynecologic Oncology. 40. 29-33 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshikatsu Nobunaga: "Differential diagnosis between complete mole and hydropic abortus by deoxyribonucleic acid fingerprints" Am.J.Obstet.Gynec.163. 634-638 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chihiro Azuma: "Studies on the pathogenesis of choriocarcinoma by analysis of restriction fragment length polymorphisms" Cancer Research. 50. 488-491 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 信永 敏克: "分子生物学的手技を用いた胞状奇胎の発生病理の解析" 産婦人科の進歩. 41. 161-163 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 東 千尋: "産婦人科領域への分子生物学の応用ーバイオテクノロジ-を用いた診断と治療ーDNA指紋を用いた多胎の卵性診断" 産科と婦人科. 56. 2377-2382 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] 佐治 文隆: "産婦人科領域への分子生物学の応用ーバイオテクノロジ-を用いた診断と治療ー診断・治療への展望" 産科と婦人科. 56. 2361-2366 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masahiko Takemura: "Determination of fetal sex from maternal blood by DNA amplication" Medical Reproductive Immunology. 135-138 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chihiro Azuma: "RFLPs analysis of extranuclear mithochondrial DNA of complete mole by using gene amplication method of PCR" Proceeding of the 4th Annual meeting of Japan Society for Basic Reproductive Immunology. 100-104 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fumitaka Saji: "A new approach using DNA fingerprinting for the determination of androgenesis as a cause of hydatidiーform mole" Placenta. 10. 399-406 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chihiro Azuma: "Zygosity Determination of multiple pregnancy by DNA fingerprinting" Am.J.Obstet.Gynec.160. 734-736 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Chihiro Azuma: "Site specific mutagenesis of human chorionic gonadotropin(hCG)beta subunit;influence of mutation on hCG production" The Journal of Molecular Endocrinology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Fumitaka Saji: "Synthesis of functional human chorionic gonadotropin by α and β subunit mRNA in Xenopus leavis oocytes" Hormone and Metaboric Research. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshihiro Tokugawa: "Analysis of site-directed mutagenesis of hCG subunit" Journal of Reproductive Immunology suppl.204 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshihiro Tokugawa: "Effect of hCG production of point mutation in CAGY region of hCG β gene." 7th International Congress of Immunology ABSTRUCT. 836 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshihiro Tokugawa: "Production of hCG from α subunit mRNA and site-specific mutant β subunit mRNA" Japanese Society of Basic Reproductive Immunology. 116-121 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] M.Mochizuki,R.Hussa(eds.): "Placental Protein Hormones. Analysis of restriction fragment length polymorphism in trophoblastic tumors." Excerpa Medica., 335 (1988)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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