| Project/Area Number |
01480398
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
TAMADA Taro Dept of Obstet Gynecol, Professor, 医学部, 教授 (60048949)
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| Co-Investigator(Kenkyū-buntansha) |
MOTOYAMA Mitsuhiro Dept of Obstet Gynecol, Instructor, 医学部, 助手 (60210100)
CHIKAZAWA Koshiro Dept of Obstet Gynecol, Instructor, 医学部, 助手 (30171929)
AKABORI Akio Dept of Obstet Gynecol, Associate Professor, 医学部, 講師 (30095004)
ARAKI Shigeo Dept of Obstet Gynecol, Associate Professor, 医学部, 助教授 (20102254)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | hypothalamus / GnRH / GHRH / CRH / hypothalamic hormone / primate / LHーRH |
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| Research Abstract |
We have developed a new method for monitoring the releasing patterns of the hypothalamic hormones in conscious monkeys. Female castrated monkeys (Macaca fuscata) were used after implamtation of a silasdc capsule containing estradiol. Under anesthesia, the orbital contents were removed. A double lumen needle was inserted along the medial wall of the superior orbital fissure into the upper part of the pituitary adjacent to the stalk. Heparinized saline was constantly fed into the infusion tube at a rate of 1.6ml / 15min. Every 15-min faction for a consecutive 12-h or longer period was analyzed for hypothalamic hormones by a RIA. Blood samples obtained before and during the experiment also were analyzed for GH and cortisol by a RIA.
Growth hormone - releasing hormone (GHRH) often increased from base levels to a peak. GHRH pulses in all animals were 60 in 84h. Themean (<plus-minus>SEM) GHRHpulseintervalwas79.5<plus-minus>6.3min. ThemeanpeakievelofGHRHpulseswas<plus-minus>2. lpg/15min, rangi
… More
ng from7. OtolO5pg/15min. The intervals between major GHRH pulses (peak value>15pg/15min ; increment>lopg/ 15min) were 148.0<plus-minus>16.4min, ranging from 30 to 300min. Seventy - six percent of GH pulses were found to synchronize with the GHRH pulses. Plasma cortisol was remarkably increased during the perfusate collections, suggesting surgical stress. Our findings suggest that the changes of the hypothalamic hormone in the perfusate collected by our procedure represent its secretary patterns from the hypot
In other groups of animals the similar experiments have been performed to elucidate the releasing patterns of corticotropin-releasing hormone (CRID and gonadotropin - releasing hormone (ONRH) in monleys under the same experimental conditions. Female castrated monkeys, were used after sc implantation of a silastic capsule containing estradiol.
Although several methods have been developed for assessing the releasing pattems of hypothalamic hormones, such as portal blood collection (Carmel et al., 1976 ; Neill et al., 1977), the push - pull perfusion of the hypothalamus (Levine et al., 1982), some technical and theoretical problems restrict their wide use. Our technique, developed as an alternative method, permits the hypothalamic hormonal release in unanesthesized animals. Less
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