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正常意識下の霊長類における視床下部ホルモンの分泌動態の解明

Research Project

Project/Area Number 01480398
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionJichi Medical School

Principal Investigator

TAMADA Taro  Dept of Obstet Gynecol, Professor, 医学部, 教授 (60048949)

Co-Investigator(Kenkyū-buntansha) MOTOYAMA Mitsuhiro  Dept of Obstet Gynecol, Instructor, 医学部, 助手 (60210100)
CHIKAZAWA Koshiro  Dept of Obstet Gynecol, Instructor, 医学部, 助手 (30171929)
AKABORI Akio  Dept of Obstet Gynecol, Associate Professor, 医学部, 講師 (30095004)
ARAKI Shigeo  Dept of Obstet Gynecol, Associate Professor, 医学部, 助教授 (20102254)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1991: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordshypothalamus / GnRH / GHRH / CRH / hypothalamic hormone / primate / LHーRH
Research Abstract

We have developed a new method for monitoring the releasing patterns of the hypothalamic hormones in conscious monkeys. Female castrated monkeys (Macaca fuscata) were used after implamtation of a silasdc capsule containing estradiol. Under anesthesia, the orbital contents were removed. A double lumen needle was inserted along the medial wall of the superior orbital fissure into the upper part of the pituitary adjacent to the stalk. Heparinized saline was constantly fed into the infusion tube at a rate of 1.6ml / 15min. Every 15-min faction for a consecutive 12-h or longer period was analyzed for hypothalamic hormones by a RIA. Blood samples obtained before and during the experiment also were analyzed for GH and cortisol by a RIA.
Growth hormone - releasing hormone (GHRH) often increased from base levels to a peak. GHRH pulses in all animals were 60 in 84h. Themean (<plus-minus>SEM) GHRHpulseintervalwas79.5<plus-minus>6.3min. ThemeanpeakievelofGHRHpulseswas<plus-minus>2. lpg/15min, rangi … More ng from7. OtolO5pg/15min. The intervals between major GHRH pulses (peak value>15pg/15min ; increment>lopg/ 15min) were 148.0<plus-minus>16.4min, ranging from 30 to 300min. Seventy - six percent of GH pulses were found to synchronize with the GHRH pulses. Plasma cortisol was remarkably increased during the perfusate collections, suggesting surgical stress. Our findings suggest that the changes of the hypothalamic hormone in the perfusate collected by our procedure represent its secretary patterns from the hypot
In other groups of animals the similar experiments have been performed to elucidate the releasing patterns of corticotropin-releasing hormone (CRID and gonadotropin - releasing hormone (ONRH) in monleys under the same experimental conditions. Female castrated monkeys, were used after sc implantation of a silastic capsule containing estradiol.
Although several methods have been developed for assessing the releasing pattems of hypothalamic hormones, such as portal blood collection (Carmel et al., 1976 ; Neill et al., 1977), the push - pull perfusion of the hypothalamus (Levine et al., 1982), some technical and theoretical problems restrict their wide use. Our technique, developed as an alternative method, permits the hypothalamic hormonal release in unanesthesized animals. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report

Research Products

(16 results)

All Other

All Publications (16 results)

  • [Publications] 荒木 重雄: "A prolonged monitoring of the hypothalamic hormonal release in monkeys(Macaca fuscata):Releasing patterns of growth hormoneーreleasing hormone" 内分泌学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 荒木 重雄: "A prolonged monitoring of the hypothalamic hormonal release in monkeys(Macaca fuscata).Releasing patterns of corticotropinーreleasing hormone(CRH) and gonadotropinーreleasing hormone(GnRH)" 内分泌学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Baldwin, D. M: "The effect of glucocorticoids on estrogen dependent luteinizing hormone release in the ovariectomized rat and on gonadotropin secretion in the intact rat." Endocrinology. 105. 120-128 (1970)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Baldwin, D. M and Sawyer, C. H.: "Effects of dexamethasone on LH release and ovulation in the cycling rat." Endocrinology. 94. 1393-1403 (1974)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Bullen, B. A., et al: "Induction of men-strual disorders by strenuous exercise in untrained women." N. Engl. J. Med.312. 1349-1353 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Carmel, P. W., et al: "Pituitary stalk portal blood in rhesus monkeys : evidence for pulsatile release of gonadotropin releasing-hormone (GnRH)." Endocrinology. 99. 243-248 (1976)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Charpenet, G., et al: "Effects of chronic intermittent immobilization stress on rat testicular androgenic function." Endocrinology. 109. 1254-1258 (1981)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Clarke, I. F. and Cummins, J. T.: "The temporal relationship between gonadotropinreleasing hormone secretion in ovariectomized ewes." Endocrinology. 111. 1737-1739 (1982)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Frisch, R. E., Wyshak, G. and Vincent.: "Delayed memarche and amenorrhea in ballet dancers." N. Engl. J. Med.303. 17-19 (1980)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 荒木 重雄: "A prolonged monitoring of the hypothalamic hormonal release in monkeys(Macaca fuscata):Releasing patterns of growth hormoneーreleasing hormone" 内分泌学会誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 荒木 重雄: "A prolonged monitoring of the hypothalamic hormonal release in monkeys(Macaca fuscata).Releasing pattern of corticotropin relcasing hormone(CRH) and gonadotropinーreleasing hormone(GnRH)" 内分泌学会誌.

    • Related Report
      1991 Annual Research Report
  • [Publications] 荒木 重雄: "A prolonged monitoring of the bypothalamic hormonal release in monkeys (Macaca fuscata):Releasing potterns of growth hormoneーreleasing hormone" 内分泌学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] 荒木 重雄: "A prolonged monitoring of the hypothalamic hormonal release in monkeys (Macaca fuscata).Releasing patterns of corticotropinーreleasing hormone (CRH) and gonadotropinーreleasing hormone (GhRH)." 内分泌学会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] 荒木重雄 他: "Effects of ru 486 on the hypothalamic releases of corticotropin-releasing hormone(CRH)and gonadotropin-releasing hormone(GnRH)" Human Reproduction(Excerpta Media). (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 荒木重雄: "A prolonged monitoring of the hypothalamic hormonal release in monkeys(Macaca fuscata):Releasing patterns of growth hormone-releasing hormone" 内分泌学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 荒木重雄: "A prolonged monitoring of the hypothalamic hormanal release in monkeys(Macaca fuscata).Releasing patterns of corticotropin-releasing hormone(CRH)and gonadotropin-releasing hormone(GnRH)" 内分泌学会誌.

    • Related Report
      1989 Annual Research Report

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Published: 1989-03-31   Modified: 2016-04-21  

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