| Project/Area Number |
01480399
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Saitama Medical School |
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Principal Investigator |
KINOSHITA Katsuyuki Saitama Medical School Dept. of Ob. & Gyn, Professor, 医学部, 教授 (80010354)
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| Co-Investigator(Kenkyū-buntansha) |
IHNO Yoshiaki Saitama Medical School Dept. of Ob. & Gyn, Lecturer, 医学部, 助手 (20211021)
TAKADA Shinichi Saitama Medical School Dept. of Ob. & Gyn, Lecturer, 医学部, 助手 (00211334)
SEKI Hiroyuki Saitama Medical School Dept. of Ob. & Gyn, Assistant Professor, 医学部, 講師 (20179328)
ISHIHARA Osamu Saitama Medical School Dept. of Ob. & Gyn, Assistant Professor, 医学部, 講師 (70176212)
TAKEDA Satoru Saitama Medical School Dept. of Ob. & Gyn, Associate Professor, 医学部, 助教授 (20143456)
小島 俊行 埼玉医科大学, 産婦人科, 講師 (90153535)
佐藤 和雄 日本大学医学部, 産婦人科, 教授 (80010180)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1991: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1989: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | decidual stromal cell / PGE_2 / cyclooxygenase / IL-1 / ararachidonic acid / 12-HETE / macrophage / inhibitor of PG biosynthesis / ILー1 / Tーcell / 脱落膜 / PG / eporygenase / lipoxygenase / cyclooxygenase / 顆粒大リンパ球 / 子宮内膜 / HETE / レクチン / 着床 / ガラクト-ス |
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| Research Abstract |
Prostaglandins (PGs) biosynthesized fran arachidonic acid in human decidua play a significant role for the implantation ell the maintenance of pregnancy. Therefore, this study revealed the part of the regulatory mechanisms of arachidmic acid metabolism in decidua. (1) endametriun forred PGE_2 at the secretory phase, whereas PG biosynthesis was inhibited in decidua. (2) The inhibitor m PG biosynthesis in decidua was purified about 15-fold, giving a preparation with a molecular weight of 55-60 KDa. (3) Human deciua was prepared by a density gradient system to obtain decidual stromal cells and macrophages, separately, in which cyclooxygenase was demonstrated by ytochemistry. (4) IL-1 gener-ated by nwrophages stimulated decidual stromal cells to induce cyclooxygenase-and to generate PGE_2. (5) Term decidual stromal cells metabolized extracellular arachidonic acid to mainly cyclooxygenase prodLxts, whereas intracellular arachidonic acid was metabolized predominantly to an epoxygenase products, together with sow lipoxygenase products. It seems likely that IL-1 and inhibitor of PG biosynthesis might be involoved in the regulation on arachidonic acid metabolism in human decidua.
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