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Lectin binding sites in the stria vascularis of normal and endolymphatic hydrops-bearing guinea pigs.

Research Project

Project/Area Number 01480408
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Otorhinolaryngology
Research InstitutionKochi Medical School

Principal Investigator

SEGUCHI Harumichi  Department of Anatomy & Cell Biol., Kochi Medical School, Professor, 医学部, 教授 (90030866)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Toshihiro  Department of Anatomy & Cell Biol., Kochi Medical School, Assist. Prof., 医学部, 助手 (40153621)
OKADA Teruhiko  Department of Anatomy & Cell Biol., Kochi Medical School, Assoc. Prof., 医学部, 助教授 (00025628)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1991: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsStria vascularis / Lectins / Carbohydrates / Cationized ferritin / Apical vesicles / Endosomes / Na-K-ATPase / Guinea pig / 内リンパ水腫 / 内耳 / 吸収 / 分泌 / 内リンパ
Research Abstract

In order to clarify the function of the marginal cells in the guinea pig stria vascularis, the following three experiments were performed :
1. Lectins were employed to determine the localization of specific carbohydorates on thin sections of Lowicryl K4M embedded materials. SBA, HPA and RCA-II labeling (Gal-NAc and Gal) was observed in many apical vesicles, endosomes and apical tubules as well as on the microvilli and micropinocytotic invaginations of the luminal plasma membrane of the marginal cells. LFA labeling (sialic acid) was found on the plasma membrane of the basal infoldings of the marginal cells. WGA (Glu-NAc) was detected along the plasma membrane of all types of cells constituting the stria vascularis. The fact that the membranes of internalization, many of the apical vesicles, endosomes and apical tubules in the marginal cells possess the same carbohydrate moieties suggested that these structures. may be associated together.
2. The uptake of cationized ferritin (CF) by the a … More pical vesicles in the marginal cells of the stria vascularis was electron microscopically studied. CF particles were found in clusters, scattered on the free surface plasma membrane facing the endolymph and in the caveolae of the marginal cells. They were also detected in the apical vesicles, coated vesicles and endosomes. These findings suggested that the apical vesicles are associated with the free surface plasma membrane, and that they are related to the efficient exchange of ions between the endolymph and the marginal cells.
3. The effect of endolymphatic hydrops on the Na-K-ATPase activity in the stria vascularis was cytochemically investigated one year after experimental induction. The morphological observations revealed intercellular dropsy and shrinkage of the basal infoldings of the marginal cells, and shrinkage and disappearance of intermediate cells. In spite of such morphological alterations, no remarkable difference was found, as regards the localization of the Na-K-ATPase activity, between the striae vasculares of hydrops and control specimens. Less

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (49 results)

All Other

All Publications (49 results)

  • [Publications] 瀬口 春道: "モルモット血管条のレクチン結合部位" Equilibrium Res.Suppl.5. 78-83 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 西山 正司: "モルモット血管条の機能とレクチンレセプタ-" Equilibrium Res.Suppl.6. 40-46 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishiyama,S.: "Carbohydrates in the guinea pig stria vascularis demonstrated with lectin-gold techniques" Acta Otolaryngol.(Stockh.). 111. 1041-1050 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Seguchi,H.: "Biochemical and cytochemical study of trimetaphosphatase activity in mammalian tissues" Acta Histochem.Cytochem.25. 51-57 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishiyama,S.: "Na-K-ATPase activity in the guinea pig stria vasculais in ex-perimentally induced endolymphatic hydrops" Hearing Res.(1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Seguchi,H.: "Ultracytochemical localization of Ca -dependent adenosine tri-phospatase and ouabain-sensitive K-dependent p-nitrophenyl-phosphatase activeties in the organ of Corti of the guinea big cochlea" Acta Otolaryngol.(Stockh.). (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 瀬口 春道: "組織学・組織化学的アプロ-チ(内耳)" (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Kobayashi, H. Seguchi, S. Nishiyama, T. Okada: "Localization of tnmetaphosphatase activity in epithelium of the guinea pig cochlear duct." Equilibrium Res.Suppl. 5. 65-71 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Seguchi, T. Kobayashi, E. Garcia, S. Nishiyama: "Lectin binding sites in the guinea pig stria vascularis." Equilibrium Res.Suppl. 5. 78-83 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Seguchi, T. Kobayashi, S. X. Zhang, T. Okada: "Biochemical characterization and cytochemical localization of rat and guinea pig trimetaphosphatase." Histochem. Cytochem.1989. pp 19-20 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Nishiyama, H. Seguchi, E. Garcia: "The fuction and lectin receptors in the guinea pig stria vasularis." Equilibrium Res.Suppl. 6. 40-46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. X. Zhang, H. Seguchi, Y. J. Piao: "Trimetaphosphatase (TMPase) - Ultracytochemical marker enzyme for lysosome." Bull. 1st Militar Med. Sch.Suppl.467-471 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Nishiyama, E. Garcia del Saz, H. Seguchi: "Carbohydrates in the guinea pig stria vascularis demonstrated with lectin-gold techniques." Acta Otolaryngol. (Stock.). 111. 1041-1050 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Seguchi, T. Kobayashi, T. Okada, S. X. Zhang, S. Nishiyama, E. Garcia del Saz: "Biochemical and cytochemical study of trimetaphosphatase activity in mammalian tissues." Acta Histochem. Cytochem.25. 51-57 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] S. Nishiyama, T. Okada, T. Kobayashi, E. Garcia del Saz, H. Seguchi: "Na-K-ATPase activity in the gunea pig stria vascularis in experimentally induced endolymphatic hydrops." Hearing Res.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Seguchi, T. Kobayashi, T. Okada, E. Garcia del Saz, S. Nishiyama: "Ultracytochemical localization of Ca-dependent adenosine triphosphate and ouabain-sensitive K-dependent p-nitrophenyl phosphatase activities in the organ of Corti of the guinea pig cochlea." Acta Otolaryngol. (Stock.). (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishiyama,S.: "Carbohydrates in the guinea pig stria vascularis demonstrated vith lectinーgold techniques" Acta Otolaryngol.(Stockh.). 111. 1041-1050 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seguchi,H.: "Biochemical and cytochemical study of trimetaphosphatase activity in mammalian tissues" Acta Histochem.Cytochem.25. 51-57 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishiyama,S.: "NaーKーATPase activity in the guineaーpig stria vascularis in experimentally induced endolymphatic hydrops" Hearing Res.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Seguchi,H.: "Ultracytochemical localization of Ca^<++>ーdependent adenosine triーphosphatase and ouabainーsensitive K^+ーdependent Pーnitrophenylーphoshatase activities in the organ of Corti of the guinea pig cochlea" Acta Otolaryngol.(Stockh.). (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kobayshi,T.: "Ultracytochemical study of the trimetaphosphatase activity in the spiral ligament of the guinea pig inner ear" Hearing Res.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 瀬口 春道: "組織学・組織化学的アプロ-チ(内耳)" 朝倉書店, (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 瀬口 春道: "内耳血管条辺縁細胞の核上部細胞質内の小胞について" 厚生省特定疾患前庭機能異常調査研究班平成2年度報告書.

    • Related Report
      1990 Annual Research Report
  • [Publications] 瀬口 春道: "内リンパ水腫モルモット内耳血管条のNaーKーATPase活性" 厚生省特定疾患前庭機能異常調査研究班平成2年度報告書.

    • Related Report
      1990 Annual Research Report
  • [Publications] 西山 正司: "モルモット血管条の機能とレクチンリセプタ-" Equilibrium Res.Suppl.6. 40-46 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Nishiyama: "Carbohydrates in the guinea pig stria vascularis demonstrated with lectinーgold techniques." Acta Otolaryngol.

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Nishiyama: "NaーKーATPase activity in the stria vascularis of endolymphatic hydrops animals." Hearing Res.

    • Related Report
      1990 Annual Research Report
  • [Publications] 瀬口 春道: "組織学・組織化学的アプロ-チ(平衡聴覚器)" 朝倉書店,

    • Related Report
      1990 Annual Research Report
  • [Publications] 小川恭弘: "Zー100の抗腫瘍効果に関する実験的研究 第2報 放射線治療とZー100の長期間投与の併用効果について" 日癌治. 24. 51-56 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Taniguchi,H.: "Production of anti-insulin monoclonal antibody and its application to immuno-assay of insulin and immunohistochemistry" Diabetes Res.Clin.Pract.6. 9-15 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Seguchi,H.: "Three dimensional observations of the palisade-shaped nerve endings of normal hair of rat's snout" Anat.Anz.169. 225-234 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林俊博: "内耳蝸牛管壁におけるtrimetaphosphatase活性の局在について" Equilibrium Res.Suppl.5. 65-71 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 瀬口春道: "モルモット血管条のレクチン結合部位" Equilibrium Res.Suppl.5. 78-83 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 瀬口春道: "新生毛細血管の形成における細胞外基質と内皮細胞の働き" Mebio. 7. 128-129 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogawa,Y.: "Application of the immunohistochemical method as a predictive assay in radiotherapy of squamous cell carcinoma of oropharynx and hypopharynx" Oncology. 47. 155-159 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 瀬口春道: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 10 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林俊博: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 7 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 岡田暉彦: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 5 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 小林俊博: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 7 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Zhang,S.X.: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 12 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Zhang,S.X.: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 12 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 瀬口春道: "文部省科学研究費 移行上皮細胞の細胞膜と細胞骨格の免疫組織細胞化学的研究研究班 昭和63年度研究報告書" 瀬口春道, 3 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] 瀬口春道: "厚生省特定疾患 前庭機能異常調査研究班 昭和63年度研究報告書" 北原正章, 4 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 瀬口春道: "厚生省特定疾患 前庭機能異常調査研究班 昭和63年度研究報告書" 北原正章, 4 (1989)

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      1989 Annual Research Report
  • [Publications] 小川恭弘: "がん治療のあゆみ" 癌徳化学療法社, 10 (1989)

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      1989 Annual Research Report
  • [Publications] Kamiike,O.: "Hyperthermic Oncology 1988" MAG.Bros.Inc., 2 (1989)

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      1989 Annual Research Report
  • [Publications] Seguchi,H.: "Histochemistry and Cytochemistry 1989" Chinese Society for Histochemistry and Cytochemistry, 2 (1989)

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      1989 Annual Research Report
  • [Publications] 瀬口春道: "組織細胞化学の技術、細胞膜" 朝倉書店, 7 (1989)

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      1989 Annual Research Report
  • [Publications] 瀬口春道: "組織細胞化学の技術、細胞膜" 朝倉書店, 14 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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