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Studies on the pathogenesis of periodontal disease from the viewpoint of local immunity

Research Project

Project/Area Number 01480430
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionOsaka University

Principal Investigator

HAMADA Shigeyuki  Osaka University Faculty of Dentistry, Professor, 歯学部, 教授 (60028777)

Co-Investigator(Kenkyū-buntansha) OGAWA Tomohiko  Osaka University Faculty of Dentistry, Associate Professor, 歯学部, 助教授 (80160761)
高田 春比古  鹿児島大学, 歯学部, 教授 (30135743)
向井 徹  大阪大学, 歯学部, 助手 (50209970)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1991: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsPeriodontal disease / Porphyromanas gingivalis / Fimbriae / Immunoglobulin / Local Immunity / Cytokine / Adjuvant / 抗体産生細胞 / ELISA / ELISPOT / 免疫応答 / 線毛タンパク / クラス / サブクラス / Hー2ハプロタイプ / Bacteroides / 菌体成分 / 抗体
Research Abstract

The dynamics of tlie host immune response to periodontal bacteria not only may be informative from the standpoint of specific mucosal protection to these pathogens, but also may reveal the capacity of the mucosal immune response to provide protection of the host. To this end, we have examined the immune response to chromatographically purified fimbriae of P. gingivalis administered orally or systemically with liposomes and adjuvant in BALB/c mice, high responders to this antigen.
Oral administration of P. giizgivalis fimbriae clearly enhanced the fimbriae-specific salivary IgA response. ELISPOT analysis revealed that significant numbers of fimbriae-specific IgA SFC were seen in lamina propria and mesenteric lymph nodes but not in Peyees patches of rice immunized orally. In contrast, antigen-specific IgM and IgG SFC were seen mainly in the circulating blood mononuclear cells. On the other hand, subcutaneous injection of fimbriae with GM-53 also raised the fimbriae-specific IgG followed by IgM and IgA responses in serum, and both IgA and IgG responses in saliva. Oral immunization was less effective than subcutaneous injection in terms of tlie serum antibody response. However, the salivary antibody level of niece injected subcutwieously was similar to that of mice immunized orally. In the subcutaneously immunized mice, fimbriae-specific SFC were detected in the spleen, blood, and brachial lymph nodes by ELISPOT assay. Fimbriae-specific IgM SFC appeared earlier and antigen-specific IgG SFC were seen later. These results show that the combined use of fimbriae together with the adjuvant results in sharply increased IgA responses in saliva wid IgG responses in serum. In summary, it is clear that the nature of tlie host's antibody response in serum and mucosal secretions are distinct, and depend on tlie route of antigen administration, the use of adjuvant and/or liposomes, and the temporal phase of the humoral inunune response following vwious immunization regimes.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Ogawa,T.: "Analysis of human IgG and IgA subclass antibody-secreting cells from localized chronic inflammatory tissue" J.Immunol.142. 1150-1158 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa,T.: "Bacteroides-specific IgG and IgA subclass antibody-secreting cells isolated from chronically inflamed gingival tissues" Clin.Exp.Immunol.76. 103-110 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa,T.: "Bacteroides gingivalis-specific serum IgG and IgA subclass antibodies in periodontal diseases" Clin.Exp.Immunol.82. 318-325 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa,T.: "Humoral immune response to Bacteroides gingivalis fimbrial antigen in mice" Immunology. 69. 8-13 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa,T.: "Porphyromonas gingivalis specific serum IgG and IgA antibodies originate from immunoglobulin-secreting cells in inflamed gingiva" Clin.Exp.Immunol.83. 237-244 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa,T.: "Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species" Oral Microbiol.Immunol.6. 332-340 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hamada,S.: "Periodontal Disease:Pathoge and Host Immune Responses" Quintessence Publiching Co.,Ltd., 410 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa, T., Tarkowski, A., McGhee, M. L., Moldoveanu, Z., Mestecky, J., Hirsch, H. Z., Koopman, W. J., Hamada, S., McGhee, J. R. and Kiyono, H.: "Analysis of human IgG and IgA subclass antibody-secreting cells from localized chronic inflammatory tissue." J. Immunol.142. 1150-1158 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa, T., McChee, M. L., Moldoveanu, Z., Hamada, S., Mestecky, J. McGhee, J. R. and Kiyono, H.: "Bacteroides-specific IgG and IgA subclass antibody-secreting cells isolated from chronically inflamed gingival tissues." Clin. Exp. Immunol.76. 103-110 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa, T., Shimauchi, H. and Hamada, S.: "Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally." Infect. Immun.57. 3466-3471 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa, T., Shimauchi, H., Kusumoto, Y and Hamada, S.: "Humoral immune response to Bacteroides gingivalis fimbrial antigen in mice." Immunology. 69. 8-13 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa, T., Kusumoto Y., Hamada, S., McGhee, J. R. and Kiyono, H.: "Bacteroides gingivalis-specific serum IgG and IgA subclass antibodies in periodontal diseases." Clin. Exp. Immunol.82. 318-325 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa, T., Kono, Y., McGhee, M. L., McGhee, J. R., Roberts, J. E., Hamada, S., and Kiyono, H.: "Porhyromonas gingivalis-specific serum IgG and IgA antibodies originate from immunoglobulin-secreting cells in inflamed gingiva." Clin. Exp. Immunol.83. 237-244 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shimauchi, H., Ogawa, T. and Hamada, S.: "Immune response gene regulation of the humoral immune response to Porphyromonas gingivalis fimbriae in mice." Immunology. 74. 362-364 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ogawa,T.: "Analysis of human IgG and IgA subclass antibodyーsecreting cells from localized chronic inflammatory tissue" J.Immunol.142. 1150-1158 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ogawa,T.: "Bacteroidesーspecific IgG and IgA subclass antibodyーsecreting cells isolated from chronically inflamed gingival tissues" Clin.Exp.Immunol.76. 103-110 (1989)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ogawa,T.: "Bacteroides gingivalisーspecific serum IgG and IgA subclass antibodies in periodontal diseases" Clin,Exp.Immunol.82. 318-325 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ogawa,T.: "Humoral immune response to Bacteroides gingivalis fimbrial antigen in mice" Immunology. 69. 8-13 (1990)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ogawa,T.: "Porphyromonas gingivalis specific serum IgG and IgA antibodies originate from immunoglobulinーsecreting cells in inflamed gingiva" Clin.Exp.Immunol.83. 237-244 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Ogawa,T.: "Distribution and immunochemical specificities of fimbriae of Porphyromonas gingivalis and related bacterial species" Oral Microbiol.Immunol.6. 332-340 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hamada,S.: "Periodontal Disease:Pathogen and Host Immune Responses" Quintessence Publiching Co.,Ltd., 410 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 小川 知彦: "歯周炎病巣における局所免疫応答" 歯科ジャ-ナル. 31. 880-888 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ogawa,T.: "Bacteroides gingivalisーspecific serum IgG and IgA subclass antibodies in periodontal diseases" Clin.Exp,Immunol.82. 318-325 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ogawa,T.: "Humoral immune response to Bacteroides gingivalis fimbrial antigen in mice" Immunology. 69. 8-13 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Fujiwara,T.: "Chemical,immunobiological and antigenic characterizations of lipopolysaccharides from Bacteroides gingivalis strains" J.Gen.Microbiol.136. 319-326 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takada,H.: "Bacteroides liopopolysaccharide (LPS) induce anaphylactoid and lethal reactions in LPSーresponsive and ーnonresponsive mice primed with MDP" J.Infect.Dis.162. 428-434 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hamada,S.: "Antimicrobial activities of thiolactomycin against gramーnegative anaerobes associated with periodontal disease" Oral Microbiol.Immunol.5. 340-345 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hamada,S: "Periodontal Disease:Pathogens and Host Immune Responses" Quintessence Publishing Co.,Ltd., 410 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ogawa,T.: "Bacteroides-specific IgG and IgA subclass antibody-secreting cells isolated fron chronically inflamed gingival tissues" Clin.Exp.Immunol.76. 103-110 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogawa,T.: "Analysis of human IgG and IgA subclass antibody-secreting cells from localized chronic inflammatory tissue" J.Immunol.142. 1150-1158 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ogawa,T.: "Mucosal and systemic immune responses in BALB/c mice to Bacteroides gingivalis fimbriae administered orally" Infect.Immun.57. 3466-3471 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hamada,S.: "Lipopolysaccharides of oral anaerobes associated with chronic inflammation:chemical and immunomodulating properties." Internat.Rev.Immunol.5. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 浜田茂幸: "歯周病は感染症か?" 日本歯科評論. 568. 67-75 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 浜田茂幸: "口腔内の嫌気性グラム陰性桿菌" 化学療法の領域. 6. 91-104 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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