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Role of Inhibitors in Bacterial Aggregation by Bacteroides Gingivalis

Research Project

Project/Area Number 01480482
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 小児・社会系歯学
Research InstitutionOsaka University

Principal Investigator

TSUNEMITSU Akira  Osaka Univ. Fac. of Dent. Prof., 歯学部, 教授 (40034160)

Co-Investigator(Kenkyū-buntansha) IWAKURA Katsuko  Osaka Univ. Fac. of Dent. Res. Asst., 歯学部, 助手 (60168549)
AMANO Atsuo  Osaka Univ. Fac. of Dent. Res. Asst., 歯学部, 助手 (50193024)
TAKESHITA Tetsuo  Osaka Univ. Fac. of Dent. Res. Asst., 歯学部, 助手 (10163396)
INOSHITA Eiji  Osaka Univ. Fac. of Dent. Res. Asst., 歯学部, 助手 (00135724)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1990: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1989: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsBacteroides gingivalis / Bacterial aggregation / Histatin / Fibrinogen / バクテロイデキジンジバリス / バクテロイデス・ジンジバリス / 血漿 / 唾液
Research Abstract

In this study, we explored the effects of human saliva and plasma on the coaggregation of Bacteroides gingivalis and Streptococcus Bmitis. We also investigated the mechanism on inhibition of the coaggregation by inhibitors such as histatin and fibrinogen. Human plasma and saliva were able to inhibit the coaggregation in a dose dependent reaction. Histidine-rich peptide as the potent inhibitor was purified from human parotid saliva. The peptide contained 12 amino acid residues whose amino acid sequence was unexpectedly the same as histatin 8. The histain was able to bind to B. Gingivalis but not S. Mitis cells. The number of histatin binding sites per cell was 36,000, and the dissociation constant was on the order of 10^<-16> M. Fibrinogen was the most potent inhibitor of the plasma-derived proteins tested. Preincubation of either B. Gingivalis or S. Mitis with fibrinogen disappear e inhibitory effect of fibrinogen. When several thiol-protease inhibitors was added to the preincubation mixture with B. Gingivalis, the inhibition of fibrinogen reappeared. While addition the protease inhibitors to the preincubation mixture with S. Mitis did not affect the coaggregation activity. Fibrinogen was also able to bind B. Gingivalis but not S. Mitis cells. Furthermore, the coaggregation and the binding of fibrinogen to B. Gingivalis cells were inhibited by L-arginine and L-lysine, although these reactions were not unaffected by the sugars tested. Thus, the inhibition of coaggregation by histatin and fibrinogen caused by binding of both inhibitors to B. Gingivalis, and may be involved in cationic amino acid residues in molecule of the inhibitors.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] 永田 英樹: "Bacteroides gingivalisとStretrococcus mitsとの菌凝集に及ぼすヒト血液および唾液の阻害効果" 口腔衛生会誌. 39. 578-579 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hideki Nagata: "Inhibitory effect of human plasma and saliva on coaggregation between <Bacteroides>___ー <gingivalis>___ー and <Streptococcus>___ー <mitis>___ー" J. Dent. Res.69. 1476-1479 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yukitaka Murakami: "Purification and characterization from human parotid secretion of a peptide which inhibits hemagglutination of <Bacteroides>___ー <gingivalis>___ー 381" FEMS Microbiology Letters. 72. 275-280 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yukitaka Murakami: "Inhibitory effects of symthetic histidineーrich peptides on hemagglutination by <Bacteroides>___ー <ginivalis>___ー 381" Archs Oral Biol.35. 775-777 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hideki Nagata: "Inhibition of coaggregation between <B. gingivalis>___ー and <S. mits>___ー by fibrinogen" J. Dent. Res.69. 1050 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yukitaka Murakami: "Inhibition of hemagglutination of <B. gingivalis>___ー by histatin in human parotid saliva" J. Dent. Res.69. 1060 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Nagata et al.: "Inhibitory effect of coaggregation between Bacteroides gingivalis and Streptococcus mitis by human plasma and saliva." J. Dent. Hlth. 39. 578-579 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Nagata et al.: "Inhibitory effect of human plasma and saliva on co-aggregation between Bacteroides gingivalis and Streptococcus mitis." J. Dent. Res. 69. 1476-1479 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Murakami et al.: "Purification and characterization from human parotid secretion of a peptide which inhibits hemagglutination of Bacteroides gingivalis 381." FEMS Microbiol. Letters. 72. 275-280 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Murakami et al: "Inhibitory effects of synthetic histidine-rich peptides on hemagglutination by Bacteroides gingivalis." Archs Oral Biol. 35. 775-777 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Nagata et al.: "Inhibition of coaggregation between B. gingivalis and S. mitis by fibrinogen." J. Dent. Res. 69. 1050 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Y. Murakami et al.: "Inhibition of hemagglutination of B. ginivalis by histatin in human parotid saliva." J. Dent. Res.69. 1060 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 高垣 勝: "ヒト顎舌下腺唾液から精製したシアロ糖タンパク質の生化学的性質とそのモノクロ-ナル抗体の作製" 口腔衛生学会雑誌. 40. 528-529 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 高垣 勝: "ヒト顎舌下腺唾液から精製したシアロ糖タンパク質に対するモノクロ-ナル抗体の応用" 口腔衛生学会雑誌. 40. 530-531 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukitaka Murakami: "Purification and characterization from human parotid secretion of a peptide which inhibits hemagglutination of <Bacteroides>___ー <gingivalis>___ー 381" FEMS Microbiology Letters. 72. 275-280 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukitaka Murakami: "Inhibitory effects of synthetic histidineーrich peptides on hemagglutination by <Bacteroides>___ー <ginivalis>___ー 381" Archs Oral Biol.35. 775-777 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideki Nagata: "Inhibition of coaggregation between <B.gingivalis>___ー and <S.mitis>___ー by fibrinogen" J.Dent.Res.69. 1050 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Yukitaka Murakami: "Inhibition of hemagglutination of B.gingivalis by histatin in human parotid saliva" J.Dent.Res.69. 1060 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 永田英樹: "Bacteroides gingivalisとStreptococcus mitisとの菌凝集に及ぼすヒト血液および唾液の阻害効果" 口腔衛生会誌. 39. 578-579 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideki Nagata: "Inhibitory effect of human plasma and saliva on coaggregation between Bacteroides gingivalis and Streptococcus mitis" J.Dent.Res.69. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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