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Studies on the Molecular Mechanisms of Regulation of Activation of Various Functions in Phagocytes

Research Project

Project/Area Number 01480490
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionHokkaido University, Faculty of Pharmaceutical Sciences

Principal Investigator

KOYAMA Jiro  Hokkaido Univ. Fac. of Pharm. Sci. Professor, 薬学部, 教授 (10025679)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Toshiyuki  Hokkaido Univ. Fac. of Pharm. Sci. Instructor, 薬学部, 助手 (90192400)
TAKAHASHI Kazuhiko  Hokkaido Univ. Fac. of Pharm. Sci. Instructor, 薬学部, 助手 (10113581)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1990: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1989: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsMacrophage / Polymorphonuclear lukocyte / Antibody / Fc receptor / NADPH oxidase / Phospholipase A_2 / Phagocytosis / Phosphatidylinositol / 多形核白亜球 / F_Cレセプタ-
Research Abstract

The molecular mechanisms by which phagocytes can recognize and eliminate antigens were studied with guinea pig macrophages and polymorphonuclear leukocytes. The main results obtained are as follows. 1. Both macrophages and polymorphonuclear leukocytes were found to possess two distinct Fc receptors : FcR_2 for IgG2, and FcR_<1,2> for both IgG1 and IgG2.2. In the phagocytosis of immune complexes of protein antigens, macrophage FcR_<1,2> operated predominantly, due to its larger number per cell than that of FcR_2, and also to existence of the intracellular pool of FcR_<1,2> For these reasons, the phagocytosis mediated by FcR_<1,2> continued at least up to 8 hr, though that by FcR_2 ceased within 2 hr. 3. The^<1,2> abilities of macrophage FcR_2 to trigger activation of the NADPH oxidasa and phospholipase A_2 were markedly higher than those of FcR_<1,2> . 4. Both the mobilization of Ca^<2+> from its intracellular pool and the phosphatidylinositol turnover were more strongly induced by FcR_2 -mediated stimulation than by FcR_<1,2> -midiated stimulation. 5. The effects of Ca^<2+> depletion with EGTA and Inomycin and of staurosporine revealed that the mechanisms of FcR_2 -and FcR_<1,2> -mediated signal transmission leading to activation of the NADPH oxidase and phospholipase A_2 differed from each other. FcR_2 triggered activation of the NADPH oxidase through the Ca^<2+>-independent pathway, though FcR_<1,2> did through two pathways : the Ca^<2+> -independent and Ca^<2+> -calmodulin pathways. In addition, the activation of phospholipase A_2 through FcR_<1,2> required the Ca^<2+> influx, though that through FcR_2 did not so. 6. The abilities of polymorphonuclear leukocyte FcR_2 to trigger activation of the NADPH oxidase and phospholipase A_2 also were higher than those of FcR_<1,2> due to different mechanisms of FcR_2 -and FcR_<1,2>-mediated signal transmission leading to activation of these enzymes.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Masaki Sato: "Characterization of the Fc receptor for IgG2 on guineapig polymorphonuclear leukocytes by the use of a monoclonal antibody" Journal of Biochemistry. 108. 66-71 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Different abilities of two types Fc_r receptor on guineaーpig macrophages to trigger the intracellular Ca^<2+> mobilization and O^ー_2 generation" Molecular Immunology. 27. 829-838 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Two distinct types of Fc receptor for IgG on guinea pigmacrophages activate the NADPH oxidase through different signalーtransduction pathways" Biochemical and Biophysical Research Communications. 172. 223-228 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Functions of two types of Fc_r receptor on guineaーpig macrophages in immune complexーinduced arechidonic acid relese" Molecular Immunology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Different abilities of two types of Fc_r receptor on guineaーpig macrophages to trigger the phosphatidylーinositol turnover" Molecular Immunology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takayuki Hida: "Different behaviour of two distinct types of Fc_r receptor on guineaーpig peritoneal macrophages during phagocytosis of soluble immune complexs" Molecular Immunology. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masaki Sato: "Characterization of the Fc receptor for IgG2 on guinea Pig polymorphonuclear leukocytes by the use of a monoclonal antibody" Journal of Biochemistry. 108. 66-71 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Different abilities of two types of Fc_r receptor on guinea-pig macrophages to trigger the intracellular Ca^<2+> mobilization and O^-_ generation" Molecular Immunology. 27. 829-838 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Two distinct types of Fc receptor for IgG on guinea Pig macrophages activate the NADPH oxidase through different signal-transduction pathways" Biochemical and Biophysical Research Communications,. 172. 223-228 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Functions of two types of Fc_r receptor on guinea-pig macrophages in immune complexinduced arachidonic acid release" Molecular Immunology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tomozumi Imamichi: "Different abilities of two types of Fc_r receptor on guinea-pig macrophages to trigger the phosphatidylinositol turnover" Molecular Immunology. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takayuki Hida,: "Different behavior of two distinct types of Fc_r receptor on guinea-pig peritoneal macrophages during phagocytosis of soluble immune complexes" Molecular Immunology,. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masaki Sato: "Characterization of the Fc receptor for IgG2 on guinea pig polymorphonuclear leukocytes by the use of a monoclonal antibody" Journal of Biochemistry. 108. 66-71 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tomozumi Imamichi: "Different abilities of two types of Fc receptor on guineaーpig macrophages to trigger the i^^rntracellular Ca^<2+> mobilization and O^ー_2 generation" Molecular Immunology. 27. 829-838 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tomozumi Imamichi: "Two distinct types of Fc receptor for IgG on guinea pig macrophages activate the NADPH oxidase through different signalーtransduction pathways" Biochemical and Biophysical Research Communications. 172. 223-228 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tomozumi Imamichi: "Functions of two types of Fc receptor on guineaーpig macrophages in immune complex^^rーinduced arachidonic acid release" Molecular Immunology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tomozumi Imamichi: "Different abilities of two types of Fc receptor on guineaーpig macrophages to trigger the p^^rhosphatidylinositol turnover" Molecular Immunology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Takayuki Hida: "Different behaviour of two distinct types of Ec_r receptor on guineaーpig peritoneal macrophages during phagocytosis of soluble immune complexes" Molecular Immunology. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideki Sato: "Relative abilities of two Fc_r receptors on guinea pig macrophages to operate in the phagocytosis of soluble immune complexes" Molecular Immunology. 26. 309-317 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Koichi Tamoto: "Phosphorylation of NADPH-cytochrome c reductase in guinea pig peritoneal macrophages stimulated with phorbol myristate acetate" FEBS Letters. 244. 159-162 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Wataru Sakamoto: "In vitro effects of vitamin E on peritoneal macrophages and T-kininogen level in rats" International Journal for Vitamin and Nutrition Research. 59. 131-139 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tomozumi Imamichi: "A Chinese traditional medicine,Juzentaihoto,inhibits the O^ー_2 generation by macrophages" Journal of Pharmacobio-Dynamics. 12. 693-699 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tomozumi Imamichi: "Different abilities of two types of Fc_r receptors on guinea-pig macrophages to trigger the intacellular Ca^<2+> mobilization and O^-_2 generation" Molecular Immunology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tomozumi Imamichi: "Different effects of cinnamic acid on the O^ー_2 generation by guinea pig macrophages stimulated with a chemotactic peptide and immune complex" Journal of Pharmacobio-Dynamics. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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