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Studies on the regulatory mechanisms and the biological significance of metabolisms of membrane phospholipids in mammalian cells by using technics of biochemical genetics.

Research Project

Project/Area Number 01480495
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionNational Institute of Health, Japan

Principal Investigator

AKAMATSU Yuzuru  National Institute of Health, Dept of Chemistry, Director, 化学部, 部長 (00072900)

Co-Investigator(Kenkyū-buntansha) KUZE Yohko  National Institute of Health, Dept of Chemistry, Senior Investigator, 化学部, 主任研究官 (50178000)
AMANO Fumio  National Institute of Health, Dept of Chemistry, Senior Investigator, 化学部, 主任研究官 (90142132)
TANAKA Yasuhito  National Institute of Health, Dept of Chemistry, Senior Investigator, 化学部, 主任研究官 (30113484)
KITAGAWA Takayuki  National Institute of Health, Dept of Chemistry, Section Chief, 化学部, 室長 (80092188)
NISHIJIMA Nasahiro  National Institute of Health, Dept of Chemistry, Section Chief, 化学部, 室長 (60072956)
久下 理  国立予防衛生研究所, 化学部, 研究員 (30177977)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1989: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsMammalian cells / Phospholipid / Mutant / CHO cell / Macrophage / Membrane permeability / Phosphatidylserine / LPS / スフィンゴミエリン / ホスファチジルイノシト-ル / アラキドン酸 / ホスホリパ-ゼA_2
Research Abstract

This research was performed mainly by using technics of biochemical genetics with variants of mammalian cells concerning metabolisms of membrane phospholipids. 1. A CHO(Chinese hamster ovary)cell mutant with altered regulation of PS(phosphatidylserine)was isolated and the existence of a regulatory factor of PS biosynthesis was suggested. 2. Gene cloning of PS synthase I and PS decarboxylase was succeeded by using a variant defective in PS synthase I, and significance of the enzyme was suggested in regulation of PS and PE(phosphatidylethanolamine)synthesis and in growth and division of cells. 3. Further. importance of PS and PE in the membrane fusion was suggested by the experiment of Sindbis virus infection to this mutant. 4. A CHO variant defective in the Na' dependent inositol transport, which is an important process in phosphatidylinositol synthesis, was also isolated, and the presence of two inositol transport systems was suggested. 5. A temperature sensitive mutant with a defect in serine-parmitoyl transferase, the rate limiting enzyme of the synthesis of sphingolipids, was isolated from CHO cells, and sphingolipids were suggested essential in the mammalian cell growth. 6. A defective mutant of PG(phosphatidylglycerol)synthase was also isolated from CHO cells and importance of PG and cardiolipin in cellular growth was also suggested. 7. A resistant mutant to high concentrations of LPS(lipopolysaccharide)was isolated from J774.1 macrophage cells, and found a defect in both arachidonate release and O_2^- generation after LPS treatment. Further, regulatory pathways from phospholipases activation was suggested to be involved in the control of LPS-induced O_2^- generation by interferon gamma. 8. Importance of calmodulin in control of the membrane permeability with extra-cellular ATP was suggested. In mouse 3T3 cells, the expression of GLUT-1, a glucose transporter gene, by TGF-beta and an altered glycosylation of GLUT-1 protein were suggested.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (209 results)

All Other

All Publications (209 results)

  • [Publications] Mure,T.: "Modulation of Membrane Permeability,Cell Proliferation and Cytotoxicity of Antitumor Agents by External ATP in Mouse Tumor Cells." Jpn.J.Cencer Res.J.Biol.Chem.83. 121-126 (1992)

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      1991 Final Research Report Summary
  • [Publications] Kitagawa,T.: "Regulation of glucose transport and the expression of glucose trans-porter gene by growth factors in mouse 3T3 cells" Keystone Symposium on Molecular and Cellular Biology,Corolade,USA.(1992)

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      1991 Final Research Report Summary
  • [Publications] Nishijima,M.: "Genetic and biochemcal approaches to understanding macrophage LPS receptor" Keystone Symposium on Recognition of Endotoxin in Biologic Systems,Lake Tahoe,California,USA.(1992)

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  • [Publications] 大塚 敏明: "CHO細胞ホスファチジルグリセロ-ル生合成欠損変異株の分離と解析" 第112回日本薬学会大会、福岡. (1992)

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  • [Publications] 西島 正弘: "マクロファ-ジ様培養細胞LPS結合能欠損変異株の損傷部位の同定" 第112回日本薬学会大会、福岡. (1992)

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  • [Publications] 益見 厚子: "TGFβ1によるSwiss 3T3細胞膜糖輸送体タンパク質合成の促進" 第112回日本薬学会大会、福岡. (1992)

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  • [Publications] 北川 隆之: "動物細胞表面膜の透過性調節機構とその薬学的応用" 第112回日本薬学会奨励賞授賞講演、福岡. (1992)

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  • [Publications] 田中 康仁: "マクロファ-ジ系細胞RAW264.7のホスホリパ-ゼA_2に対するモノクロ-ナル抗体の調製と性質" 第112回日本薬学会大会、福岡. (1992)

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  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1のPMAによる細胞接着性の誘導とその調節機構" 第112回日本薬学会大会、福岡. (1992)

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  • [Publications] 斎藤 恭子: "CHO細胞ホスファチジルセリン生合成に関与する遺伝子pssAの遺伝子産物に対する抗体の調製とこれを用いた解析" 第112回日本薬学会大会、福岡. (1992)

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  • [Publications] Amano,F.: "Genetic characterization of human KB cell lines resistant to epidermal growth factor-Pseudomonas exotoxin conjugates" J.Cell.Physiol.135. 18066-18071 (1991)

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      1991 Final Research Report Summary
  • [Publications] Kuge,O.: "A cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a chinese hamster ovary cell mutant" J.Biol.Chem.268. 6370-6376 (1991)

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      1991 Final Research Report Summary
  • [Publications] Kitagawa,T.: "Transforming growth factor-β1 stimulates glucose uptake and the expression of glucose transporter mRNA in quiescent Swiss mouse 3T3 cells" J.Biol.Chem.266. 18066-18071 (1991)

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  • [Publications] Kuge,O.: "A Chinese hamster cDNA encoding a protein essential for the phospatidylserine synthase I activity" J.Biol.Chem.266. 24184-24189 (1991)

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      1991 Final Research Report Summary
  • [Publications] Hanada,K.: "Functional reconstitution of sphingomyelin synthase in Chinese hamster ovary cell membranes" Biochim.Biophys.Acta.1086. 151-156 (1991)

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  • [Publications] Shiozaki,M.: "Synthesis of 2-Deoxy-2-〔(2,2-difluoro-3-hydroxytetradecanoyl)-amino〕-3-O-〔(R)-3-tetradecanoyloxytetradecanoyl〕-D-glucopyranose 4-phosphate" J.Med.Chem.34. 2643-2646 (1991)

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  • [Publications] Amano,F.: "A lipopolysaccharide(LPS)-resistant mutant isolated from a macrophagelike cell line,J774.1,exhibits an altered activated-macrophage phenotype in response to LPS" Infect.Immun.59. 2166-2174 (1991)

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  • [Publications] Shiozaki,M.: "Synthesis of 2-deoxy-2-〔(R)-3-hydroxytetradecanoylamino〕-3-O-〔(R)-3-hydroxytetradecanyl〕-D-glucopyranosyl-1α-and 4-phosphate" Carbohydr.Res.222. 57-68 (1991)

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  • [Publications] Shiozaki,M.: "Synthesis of 3-ether analogue of Lipid A" Carbohydr.Res.222. 69-82 (1991)

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  • [Publications] Kobayashi,Y.: "〔(3R)-3-(tetradecanoyloxy)-tetradecanoyl〕-D-glucopyranose 4-(hydrogen phosphate)and 2-Deoxy-2-〔(3R and 3S)-3-hydroxytetradecan-amido〕-3-O-〔(3R)-3-(tetradecanoyloxy)tetradecanoyl〕-a-D-glucopyranosyl fluoride 4-(dihydrogene phosphate):Fluorosugar Analogues of GLA-60" Carbohydr.Res.222. 83-97 (1991)

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  • [Publications] Hanada,K.: "Sphingolipids are Essential for the Growth of Chinese Hamster Ovary Cells:Restoration of the Growth of a Mutant Defective in Sphingoid Base Biosynthesis by Exogenous Sphingolipids."

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      1991 Final Research Report Summary
  • [Publications] 大賀 洋子: "動物培養細胞より分離したイノシト-ル輸送欠損変異株を用いたイノシト-ル輸送機構の解析" 日本膜学会第13年会、東京. (1991)

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  • [Publications] Nishijima,M.: "Genetic and molecular approaches to phosphatidylserine biosynthesis in cultured mammalian cells" Gordon Research Conferences,New Hampshire,U.S.A.(1991)

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      1991 Final Research Report Summary
  • [Publications] Iwaki,M.: "Cytotoxicity of aflatoxin B1 and its binding proteins in chicken embryo primary cultured hepatocytes" Grodon Research Conferences,New Hampshire,U.S.A.(1991)

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  • [Publications] 久下(原) 小百合: "マクロファ-ジ系細胞J774.1細胞のLPS受容体の精製と性質" 第33回脂質生化学会、京都. (1991)

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      1991 Final Research Report Summary
  • [Publications] 花田 賢太郎: "CHO細胞膜におけるスフィンゴミエリン合成酵素の機能的再構成" 第33回日本脂質生化学研究会研究集会、京都. (1991)

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  • [Publications] 天野 富美夫: "ヒトKB細胞およびEGFー毒素耐性変異株におけるグルコ-ス取り込み能のEGFによる調節" 日本癌学会第50回総会,東京. (1991)

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  • [Publications] 横尾 信夫: "ATPの抗癌剤に対する作用増強について" 第50回日本癌学会、東京. (1991)

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  • [Publications] 久下 理: "CHO細胞リン脂質・セリン交換酵素IのcDNAの単離と解析" 第64回日本生化学会大会、東京. (1991)

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  • [Publications] 田中 康仁: "各種脂肪酸修飾したマクロファ-ジ系細胞株J774.1における貪食能の変動について" 第64回日本生化学会大会、東京. (1991)

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  • [Publications] 天野 富美夫: "ザイモサン刺激したマクロファ-ジ細胞株からのアラキドン酸遊離調節機構におけるグルコ-ス等の役割" 日本生化学会第64回大会、東京. (1991)

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  • [Publications] 北川 隆之: "増殖因子による糖輸送・輸送体遺伝子発現の調節:TGFβの促進作用" 第64回日本生化学会大会、東京. (1991)

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  • [Publications] 益見 厚子: "TGFβ1によるSwiss3T3細胞の糖輸送活性及び糖輸送体タンパク質合成の促進" 第64回日本生化学会大会、東京. (1991)

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  • [Publications] 花田 賢太郎: "スフィンゴ脂質はCHO細胞の生育に必須である" 第64回日本生化学会大会、東京. (1991)

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  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1由来のLPS耐性変異株、LPS1916におけるLPSによるO_<2^ー>産生誘導の欠損とIFNγによる回復" 第13回生体膜と薬物の相互作用シンポジウム、東京. (1991)

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  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1由来のLPS耐性変異株LPS1916における02ー産生欠損とIFNーγによる回復" 第66回日本細胞学会関東支部総会、薬師寺、栃木. (1991)

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  • [Publications] 益見 厚子: "TGFβ1によるSwiss 3T3細胞の糖輸送活性および糖輸送体タンパク質合成の促進" 第13回生体膜と薬物の相互作用シンポジウム、東京. (1991)

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  • [Publications] 西島 正弘: "リン脂質・塩基交換反応" 新生化学実験講座(4)脂質IIリン脂質(東京化学同人). 349 (1991)

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  • [Publications] 西島 正弘: "遺伝生化学アプロ-チー動物細胞" 新生化学実験講座(4)脂質IIリン脂質(東京化学同人). 475 (1991)

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  • [Publications] 西島 正弘: "レプリカ法" 新生化学実験講座(18)細胞培養技術(東京化学同人). 279-284 (1991)

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  • [Publications] 西島 正弘: "動物細胞におけるホスファチジルセリンとホスフアチジルエタノ-ルアミンの代謝と意義ー遺伝生化学的解析ー" 蛋白質・核酸・酵素. 36. 554-562 (1991)

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  • [Publications] 久下 理: "セルロ-ス濾紙あるいはポリエステル布を用いた基質接着性細胞のレプリカ法" 蛋白質・核酸・酵素(別冊)分子細胞遺伝学実験法. 36. 2106-2109 (1991)

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  • [Publications] 久下 理: "cDNAライブラリ-を用いた遺伝子の導入と回収" 蛋白質・核酸・酵素(別冊)分子細胞遺伝学実験法. 36. 2271-2274 (1991)

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  • [Publications] 花田 賢太郎: "膜脂質代謝欠損変異株" 蛋白質・核酸・酵素. 36. 2180-2184 (1991)

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  • [Publications] 天野 富美夫: "EGF結合性変異株のEGF応答異常" 蛋白質・核酸・酵素 特集「細胞増殖因子の基礎と臨床」. 36. 978-985 (1991)

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  • [Publications] 天野 富美夫: "EGF" 治療学. 25. 1045-1048 (1991)

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  • [Publications] 赤松 穣: "動物細胞におけるリン脂質生合成の遺伝生化学" 日本農芸化学会誌. 66. 45-48 (1992)

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  • [Publications] 天野 富美夫: "中性脂質" 「脂質の分析法」(赤松 穣 編).

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  • [Publications] 天野 富美夫: "受容体研究法" 「細胞生物学実験法」(堀田 康雄・大熊 勝治編).

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  • [Publications] 益見 厚子: "Swiss 3T3細胞におけるTGFβ1による増殖と糖輸送活性の促進効果." 日本薬学会. (1991)

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      1991 Final Research Report Summary
  • [Publications] 花田 賢太郎: "スファンゴミエリン合成酵素の可溶化再構成." 日本薬学会. (1991)

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  • [Publications] 久下 理: "リン脂質・セリン交換酵素IのcDNAクロ-ニング." 日本薬学会第110年会. (1990)

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      1991 Final Research Report Summary
  • [Publications] 北川 隆之: "新規合成スルホンアミド誘導体のATP依存性動物細胞膜透過性変化の促進作用." 日本薬学会第110年会. (1990)

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  • [Publications] 岩城 正昭: "ニワトリ胚初代培養肝細胞のアフラトキシンB_1結合蛋白質." 日本薬学会第110年会. (1990)

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  • [Publications] Ohga,Y.: "Chinese hamster ovary cell mutants defective in myo-inositol transport." J.Biol.Chem.265. 18083-18086 (1990)

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      1991 Final Research Report Summary
  • [Publications] Hanada,K.: "A temperature-sensitive mammalian cell mutant with thermolabile serine palmitoyl transferase for the phingolipid biosynthesis." J.Biol.Chem.265. 22137-22142 (1990)

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  • [Publications] Nishizawa,N.: "Mitogenic signaling pathways of growth factors can be distinguished by the involvement of pertussis toxin-sensitive guanosine triphosphate-binding protein and of protein kinase C." Cell Regulation. 1. 747-761 (1990)

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      1991 Final Research Report Summary
  • [Publications] Tanaka,Y.: "Purification and properties of phosphatidyl-N-monometylethanolamine N-methyl transferase,the enzyme catalyzing the second and the third steps in the phosphatidylethanolamine N-methyltransferase system,from mouse liver microsomes." Jpn.J.Med.Sci.Biol.43. 59-73 (1990)

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    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hasegawa,Kazuhide: "Isolation and characterization of Chinese hamster ovary cell mutants defective in glucose-transport" Biochim.Biophys.Acta. 1051. 221-229 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hara-Kuge,Sayuri: "Isolation of an LPS-resistant mutant,with defective LPS binding,of cultured macrophage-like cells" J.Biol.Chem.265. 6606-6610 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 岩城 正昭: "トリ胚肝臓由来アフラトキシンB_1結合性蛋白質の性質およびその精製." 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 北川 隆之: "動物細胞表面膜のATPによる透過性調節と抗癌剤の作用増強." 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 天野 富美夫: "マウスマクロファ-ジ系細胞株J774.1より得られた高濃度LPS耐性変異株、LPS1916細胞における活性酵素産生異性について." 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1より分離したLPS耐性変異株、LPS1916細胞におけるアラキドン酸代謝." 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久下 小百合: "マクロファ-ジLPS受容体の遺伝生化学的解析" 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久下 理: "ホスファチジルセリン代謝に関与するチャイニ-ズ・ハムスタ-の遺伝子とcDNAの解析" 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 西島 正弘: "マクロファ-ジ系細胞株J774.1細胞のLPS結合損傷株の分離と解析" 第63回日本細菌学会総会(徳島). (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 北川 隆之: "動物細胞の増殖調節に関与する遺伝子の解析ー増殖因子による糖輸送・輸送体遺伝子の調節機構." ビオメディカ. 5. 76-81 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 川越 淳一: "細胞膜透過性変化を利用したATPによる抗癌剤の増強効果." 日本薬学会第110年会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kuge,O.: "Molecular cloning of a gene which suppresses the mutation in a phosphatidylserine-auxotroph of CHO cells." The Konrad Bloch Symposium. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kuge,O.: "Molecular cloning of a gene that complements the mutation in a phosphatidylserine-auxotrophic Chinese hamster ovary cell mutant." 31st International Conference on the Biochemistry of lipids. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 天野 富美夫: "ヒトKB細胞由来EGFー毒素結合体耐性変異株のEGFレセプタ-異常と発癌性." 第49回日本癌学会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
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      1991 Final Research Report Summary
  • [Publications] 花田 賢太郎: "スフィンゴ脂質生合成の欠損した突然変異動物細胞の単離と解析." 第63回日本生化学会大会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 花田 賢太郎: "スフィンゴ脂質生合成の欠損した突然変異細胞の単離と解析." 第32回日本脂質生化学研究会研究集会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久下 小百合: "リピドAモノサッカライド類縁体のマクロファ-ジ活性化能." 日本薬学会第110年会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 花田 賢太郎: "スフィンゴ脂質生合成の欠損した突然変異細胞の単離." 日本薬学会第110年会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 天野 富美夫: "LPSで活性化したマクロファ-ジ系細胞株J774.1におけるプロスタグランジン遊離のシクロヘキシミドによる阻害." 日本薬学会第110年会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田中 康仁: "マクロファ-ジ系細胞株RAW264.7の2ーアラキドニルPCまたはPEに特異的なホスホリパ-ゼA_2の分離・精製と性質." 日本薬学会第110年会. (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田中 康仁: "マクロファ-ジ系細胞系RAW264.7に見い出されたCa^<2+>依存性ホスホリパ-ゼA_2の部分精製と性質" 生化学. 61. 1031 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 天野 富美夫: "プロスタグランジンによるLPS活性化マクロファ-ジからのアラキドン酸遊離の調節" 生化学. 61. 934 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大賀ー久世 洋子: "CHOーK1細胞のイノシト-ル輸送の性質と輸送欠損変異株の分離" 日本薬学会第109年会. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 大賀ー久世 洋子: "CHO細胞のイノシト-ル輸送機構" 第11回生体膜と薬物の相互作用シンポジウム. (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 原ー久下 小百合: "マクロファ-ジ系細胞株J774.1細胞のLPS結合損傷変異株の分離と解析" 生化学. 61. 934 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久下 理: "動物細胞におけるホスファチジルセリンの生合成機構" 生化学. 61. 1259-1264 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 久下 理: "リン脂質ーセリン交換酵素IのcDNAクロ-ニングの試み" 生化学. 61. 883 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kuge,Osamu: "Abortive infection with Sindbis virus of a Chinese Hamster ovary cell mutant defective in phosphatidylserine and phosphatidy lethanolomine" Biochim.Biophys.Acta. 986. 61-69 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tanaka,Yasuhito: "Degradation of arachidonyl phospholipids catalyzed by two phospholipases A_2 and phospholipase C in a lipopolysaceharide-treated macrophage cell line,RAW264.7" Arch.Biochem.Biophys.272. 210-218 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hasegawa,Kazuhide: "Isolation and characterization of a Chinese Hamster overy cell mutant with altered regulation of phosphatidylserine biosynthesis" J.Biol.Chem.264. 19887-19892 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 西島 正弘: "毒性生化学(上) (一部担当)" 地人書館, (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] T. Mure, K. Sano, and Takayuki Kitagawa: "Modulation of Membrane Permeability, Cell Proliferation and Cytotoxicity of Antitumor Agents by External ATP in Mouse Tumor Cells." Jpn. J. Cancer Res.83. 121-126 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Takayuki Kitagawa: "Regulation of glucose transport and the expression of glucose transporter gene by growth factors in mouse 3T3 cells." Keystone Synposium on Molecular and Cellular Biology. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahiro Nishijima: "Genetic and biochemical approaches to understanding macrophage LPS receptor." Keystone Symposium on Recognition of Endotoxin in Biologic Systems. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] F. Amano, I. Pastan, and M. M. Gottesman: "Genetic characterization of human KB cell lines resistant to epidermal growth factor-Pseudomonas exotoxin conjugates." J. Cell. Physiol.135. 527-532 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kuge, O., Nishijima, M. and Akamatsu, Y.: "A cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a chinese hamster ovary cell mutant." J Biol Chem. 268. 6370-6376 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kitagawa, T., Masumi, A. and Akamatsu, Y.: "Transforming growth factor-beta1 stimulates glucose uptake and the expression of glucose transporter mRNA in quiescent Swiss mouse 3T3 cells." J Biol Chem. 266. 18066-18071 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kuge, O., Nishijima, M., and Akamatsu, Y.: "A Chinese hamster cDNA encoding a protein essential for the phospatidylserine synthase I activity." J Biol Chem. 266. 24184-24189 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hanada, K., Horii, M., and Akamatsu, Y.: "Functional reconstitution of sphingomyelin synthase in Chinese hamster ovary cell membranes." Biochim Biophys Acta. 1086. 151-156 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] "Synthesis of 2-Deoxy-2-[(2, 2-difluoro-3-hydroxytetradecanoyl)-amino]-3-0-[(R)-3-tetradecanoyloxytetradecanoyl]-D-glucopyranose 4-phosphate." J Med Chem. 34. 2643-2646 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Amano, F., and Akamatsu, Y.: "A lipopolysaccharide (LPS)-resistant mutant isolated from a macrophagelike cell line, J774.1, exhibits an altered activated-macrophage phenotype in response to LPS." Infect Immun. 59. 2166-2174 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiozaki, M., Kobayashi, Y. Ishida, N., Arai, M., Hiraoka, T., Nishijima, M., Kuge, S., Ohtsuka, T., and Akamatsu, Y.: "Synthesis of 2-deoxy-2-[(R)-3-hydroxytetradecanoylamino]-3-0-[(R)-3-hydroxytetradecanyl]-D-Glucopyranosyl-1alpha-and 4-phosphate." Carbohydr Res. 222. 57-68 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Shiozaki, M., Kobayashi, Y., Arai, M., Ishida, N., Hiraoka, T., Nishijima, M., Kuge, S., Ohtsuka, T., and Akamatsu, Y.: "Synthesis of 3-ether analogue of Lipid A" Carbohydr. Res. 222. 69-82 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Yoshiyuki Kobayashi, Noboru Ishida, Masami Arai, Masao Shiozaki, Tetsuo Hiraoka, Masahiro Nishijima, Sayuri Kuge, Toshiaki Ohtsuka, and Yuzuru Akamatsu: "Syhthesis of 2, 6-Dideoxy-6-fluoro-2-[(3R and 3S)-3-hydroxytetradecanamido]-30-[(3R)-3-(tetradecanoyloxy)-tetradecanoyl]-D-glucopyranose 4-(hydrogen phosphate) and 2-Deoxy-2-[(3R and 3S)-3-hydroxytetradecan-amido]-3-O-[(3R)-3-(tetradecanoyloxy) tetradecanoyl]-a-D-glucopyranosyl fluoride 4-(dihydrogene phosphate) : Fluorosugar Analogues of GLA-60." Carbohydr. Res. 222. 83-97 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kentaro Hanada, Masahiro Nishijima, M. Kiro, A. Hasegawa, S. Fujita, T. Ogawa, and Yuzuru Akamatsu: "Sphingolipids are Essential for the Growth of Chinese Hamster Ovary Cells : Restoration of the Growth of a Mutant Defective in Sphingoid Base Biosynthesis by Exogenous Sphingolipids."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masahiro Nishijima: "Genetic and molecular approaches to phosphatidylserine biosynthesis in cultured mammalian cells." Gordon Research Conferences. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Masaaki Iwaki, Kageaki Aibara, Susumu Kumagai, Yuzuru Akamatsu and Takayuki Kitagawa: "Cytotoxicity of aflatoxin B1 and its binding proteins in chicken embryo primary coltured hepatocytes" Gordon Research Conferences. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ohga, Y., Nishijima, M., Akamatsu, Y.: "Chinese hamster ovary cell mutants defective in myo-inositol transport" J. Biol. Chem.265. 18083-18086 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hanada, K., Nishijima, M., Akamatsu, Y.: "A temperature-sensitive mammalian cell mutant with thermolabile serine-palmitoyl transferase for the sphingolipid biosynthesis." J. Biol. Chem.265. 22137-22142 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Nishizawa, N., Okano, Y., Chatani, Y., Amano, F., Tanaka, E., Nomoto, H., Nozawa, Y., Kohno, M.: "Mitogenic signaling pathways of growth factors can be distinguished by the involvement of pertussis toxin-sensitive guanosine triphosphate-binding protein and of protein kinase C." Cell Regulation. 1. 747-761 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Tanaka, Y., Amano, F., Maeda, M., Nishijima, M., Akamatsu, Y.: "Purification and properties of phosphatidyl-N-monomethylethanolamine N-methyltransferase, the enzyme catalyzing the second and the third steps in the phosphatidylethanolamine N-methyltransferase system, from mouse liver microsomes." Jpn. J. Med. Sci. Biol.43. 59-73 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hasegawa, K., Anraku, Y., Kasahara, M., Akamatsu, Y., Nishijima, M.: "Isolation and characterization of Chinese hamster ovary cell mutants defective in glucose trasnport" Biochim. Biophys. Acta. 1051. 221-229 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Hara-Kuge, S., Amano, F., Nishijima, M., Akamatsu, Y.: "Isolation of a lipopolysaccharide (LPS) -resistant mutant, with defective LPS binding of cultured macrophagelike cells." J. Biol. Chem.265. 6606-6610 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Osamu Kuge, Masahiro Nishijima, and Yuzuru Akamatsu: "Molecular cloning of a gene which suppresses the mutation in a phosphatidylserine-auxotroph of CHO cells" The Konrad Bloch Symposium. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Osamu Kuge, Masahiro Nishijima, and Yuzuru Akamatsu: "Molecular cloning of a gene that complements the mutation in a phosphatidylserine-auxotrophic Chinese hamster ovary cell mutant" 31st International Conference on the Biochemistry of Lipids. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Tanaka, F. Amano, H, Kishi, M. Nishijima, and Y. Akamatsu: "Degradation of arachidonyl-phospholipids catalyzed by two phospholipases A_2 and phospholipase C in a lipopolysaccharide-treated macrophage cell line, RAW 264.7." Arch. Biochem. Biophys.277. 210-218 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Osamu Kuge, Masahiro Nishijima, and Yuzuru Akamatsu: "Abortive infection with Sindbis virus of a Chinese hamster ovary cell mutant defective in phosphatidylserine and phosphatidylethanolamine biosynthesis." Biochim. Biophys. Acta. 986. 61-69 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Kazuhide Hasegawa, Osamu Kuge, Masahiro Nishijima, and Yuzuru Akamatsu: "Isolation and characterization of a Chinese hamster ovary cell mutant with altered regulation of phosphatidylserine biosynthesis." J. Biol. Chem.264. 19887-19892 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Mure,T.: "Modulation of Membrane Permeability,Cell Proliferation and Cytotoxicity of Antitumor Agents by External ATP in Mouse Tumor Cells." Jpn.J.Cancer Res.J.Biol.Chem.83. 121-126 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kitagawa,T.: "Regulation of glucose transport and the expression of glucose trans-porter gene by growth factors in mouse 3T3 cells" Keystone Symposium on Molecular and Cellular Biology,Corolado,USA.(1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishijima,M.: "Genetic and biochemical approaches to understanding macrophage LPS receptor" Keystone Symposium on Recognition of Endotoxin in Biologic Systems,Lake Tahoe,California,USA. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 大塚 敏明: "CHO細胞ホスファチジルグリセロ-ル生合成欠損変異株の分離と解析" 第112回日本薬学会大会、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 西島 正弘: "マクロファ-ジ様培養細胞LPS結合能欠損変異株の損傷部位の同定" 第112回日本薬学会大会、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 益見 厚子: "TGFβ1によるSwiss3T3細胞膜糖輸送体タンパク質合成の促進" 第112回日本薬学会大会、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 北川 隆之: "動物細胞表面膜の透過性調節機構とその薬学的応用" 第112回日本薬学会奨励賞授賞講演、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 田中 康仁: "マクロファ-ジ系細胞RAW264.7のホスホリパ-ゼA_2に対するモノクロ-ナル抗体の調製と性質" 第112回日本薬学会大会、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1のPMAによる細胞接着性の誘導とその調節機構" 第112回日本薬学会大会、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 斎藤 恭子: "CHO細胞ホスファチジルセリン生合成に関与する遺伝子pssAの遺伝子産物の対する抗体の調製とこれを用いた解析" 第112回日本薬学会大会、福岡. (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] Amano,F.: "Genetic characterization of human KB cell lines resistant to epidermal growth factor-Pseudomonas exotoxin conjugates" J.Cell Physiol.135. 18066-18071 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kuge,O.: "A cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a chinese hamster ovary cell mutant" J.Biol.Chem.268. 6370-6376 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kitagawa,T.: "Transforming grwth factor-β1 stimulates glucose uptake and the expression of glucose transporter mRNA in quiescent Swiss mouse 3T3 cells" J.Biol.Chem.266. 18066-18071 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kuge,O.: "A chinese hamster cDNA enconding a protein essential for the phospatidyl serine synthase I activity" J.Biol.chem.266. 24184-24189 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hanada,K.: "Functional reconstitution of sphingomyelin synthase in Chinese hamster ovary cell membranes" Biochim.Biophys.Acta.1086. 151-156 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiozaki,M.: "Synthesis of 2-Deoxy-2-[(2,2-difluoro-3-hydroxytetradecanoy1)-amino]-3-O-[(R)-3-tetradecanoyloxytetradecanoy1]-D-glucopyranose 4-phosphate" J.Med.Chem.34. 2643-2646 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Amano,F.: "A lipopolysaccharide(LPS)-resistant mutant isolated from a macrophagelike cell line,J774.1,exhibits an altered activated-macrophage phenotype in response to LPS" Infect.Immun.59. 2166-2174 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiozaki,M.: "Synthesis of 2-deoxy-2-[(R)-3-hydroxytetradecanoylamino]-3-0-[(R)-3-hydroxytetradecany1]-D-glucopyranosy1-1α-and 4-phosphate" Carbohydr.Res.222. 57-68 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Shiozaki,M.: "Synthesis of 3-ether analogue of Lipid A" Carbohydr.Res.222. 69-82 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Kobayashi,Y.: "[(3R)-3-(tetradecanoyloxy)-tetradecanoyl]-D-glucopyranose 4-(hydrogen phosphate)and 2-Deoxy-2-[(3R and 3S)-3-hydroxytetradecan-amido]-3-0-[(3R)-3-(tetradecanoyloxy)tetradecanoyl]-a-D-glucopyranosyl fluoride 4-(dihydrogene phosphate):Fluorosugar Analogues of GLA-60" Carbohydr.Res.222. 83-97 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Hanada,K.: "Sphingolipids are Essential for the Growth of Chinese Hamster Hamster Ovary Cells: Restoration of the Growth of a Mutant Defective in Sphingoid Base Biosynthesis by Exogenous Sphingolipids." Submitted.

    • Related Report
      1991 Annual Research Report
  • [Publications] 大賀 洋子: "動物培養細胞より分離したイノシト-ル輸送欠損変異株を用いたイノシト-ル輸送機構の解析" 日本膜学会第13年会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Nishijima,M.: "Genetic and molecular approaches to phosphatidylserine biosynthesis in coltured mammalian cells" Gordon Research Conferences,New Hampshire,U.S.A.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] Iwaki,M.: "Cytotoxicity of aflatoxin B1 and its binding proteins in chicken embryo primary cultured hepatocytes" Gordon Research Conferences,New Hampshire,U.S.A.(1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久下(原) 小百合: "マクロファ-ジ系細胞J774.1細胞のLPS受容体の精製と性質" 第33回脂質生化学会、京都. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 花田 賢太郎: "CHO細胞膜におけるスフィンゴミエリン合成酵素の機能的再構成" 第33回日本脂質生化学研究会研究集会、京都. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "ヒトKB細胞およびEGFー毒素耐性変異株におけるグルコ-ス取り込み能のEGFによる調節" 日本癌学会第50回総会,東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 横尾 信夫: "ATPの抗癌剤に対する作用増強について" 第50回日本癌学会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久下 理: "CHO細胞リン脂質・セリン交換酵素IのcDNAの単離と解析" 第64回日本生化学会大会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 田中 康仁: "各種脂肪酸修飾したマクロファ-ジ系細胞株J774.1における貪食能の変動について" 第64回日本生化学会大会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "ザイモサン刺激したマクロファ-ジ細胞株からのアラキドン酸遊離調節機構におけるグルコ-ス等の役割" 日本生化学会第64回大会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 北川 隆之: "増殖因子による糖輸送・輸送体遺伝子発現の調節・TGFβの促進作用" 第64回日本生化学会大会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 益見 厚子: "TGFβ1によるSwiss3T3細胞の糖輸送活性及び糖輸送体タンパク質合成の促進" 第64回日本生化学会大会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 花田 賢太郎: "スフィンゴ脂質はCHO細胞の生育に必須である" 第64回日本生化学会大会、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1由来のLPS耐性変異株、LPS1916におけるLPSによるO_2ー産生誘導の欠損とIFNγによる回復" 第13回生体膜と薬物の相互作用シンポジウム、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1由来のLPS耐性変異株LPS1916における02ー産生欠損とIFNーγによる回復" 第66回日本細菌学会関東支部総会、薬師寺、栃木. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 益見 厚子: "TGFβ1によるSwiss3T3細胞の糖輸送活性および糖輸送体タンパク質合成の促進" 第13回生体膜と薬物の相互作用シンポジウム、東京. (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 西島 正弘: "リン脂質・塩基交換反応" 新生化学実験講座(4)脂質IIリン脂質(東京化学同人). 349 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 西島 正弘: "遺伝生化学アプロ-チー動物細胞" 新生化学実験講座(4)脂質IIリン脂質(東京化学同人). 475 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 西島 正弘: "レプリカ法" 新生化学実験講座(18)細胞培養技術(東京化学同人). 279-284 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 西島 正弘: "動物細胞におけるホスファチジルセリンとホスフアチジルエタノ-ルアミンの代謝と意義ー遺伝生化学的解析ー" 蛋白質・核酸・酵素. 36. 554-562 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久下 理: "セルロ-ス濾紙あるいはポリエステル布を用いた基質接着性細胞のレプリカ法" 蛋白質・核酸・酵素(別冊)分子細胞遺伝学実験法. 36. 2106-2109 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 久下 理: "cDNAライブラリ-を用いた遺伝子の導入と回収" 蛋白質・核酸・酵素(別冊)分子細胞遺伝学実験法. 36. 2271-2274 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 花田 賢太郎: "膜脂質代謝欠損変異株" 蛋白質・核酸・酵素. 36. 2180-2184 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "EGF結合性変異株のEGF応答異常" 蛋白質・核酸・酵素 特集「細胞増殖因子の基礎と臨床」. 36. 978-985 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫: "EGF" 治療学. 25. 1045-1048 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] 赤松 穣: "動物細胞におけるリン脂質生合成の遺伝生化学" 日本農芸化学会誌. 66. 45-48 (1992)

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫(赤松 穣 編): "中性脂質" 「脂質の分析法」.

    • Related Report
      1991 Annual Research Report
  • [Publications] 天野 富美夫(堀田康雄・大熊勝治編): "受容体研究法" 「細胞生物学実験法」.

    • Related Report
      1991 Annual Research Report
  • [Publications] Ohga,Y.: "Chinese hamster ovary cell mutants defective in myoーinositol transport." J.Biol.Chem.265. 18083-18086 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hanada,K.: "A temperatureーsensitive mammalian cell mutant with thermolabile serine palmitoyl transferase for the sphingolipid biosynthesis." J.Biol.Chem.265. 22137-22142 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishizawa,N.: "Mitogenic signaling pathways of growth factors can be distinguished by the involvement of pertussis toxinーsensitive guanosine triphosphateーbinding protein and of protein kinase C." Cell Regulation. 1. 747-761 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tanaka,Y.: "Purification and properties of phosphatidylーNーmonomethylethanolamine Nーmethyl transferase,the enzyme catalyzing the second and the third steps in the phosphatidylethanolamine Nーmethyltransferase system,from mouse liver microsomes." Jpn.J.Med.Sci.Biol.43. 59-73 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuge,O.: "A cloned gene encoding phosphatidylserine decarboxylase complements the phosphatidylserine biosynthetic defect of a Chinese hamster ovary cell mutant." J.Biol.Chem.,inpress.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuge,O.: "A chinese hamster cDNA encoding a protein essential for the phosphatidylserine synthase I activity." Proc.Natl.Acad.Sci.USA.

    • Related Report
      1990 Annual Research Report
  • [Publications] Amano,F.: "Isolation and characterization of a LPSーresistant mutant from a macrophgeーlike cell line,J 774.1,exhibiting an altered phenotype of activated macrophages in response to LPS." Infect.Immun.

    • Related Report
      1990 Annual Research Report
  • [Publications] 北川 隆之: "動物細胞の増殖調節に関与する遺伝子の解析ー増殖因子による糖輸送・輸送体遺伝子の調節機構." ビオメディカ. 5. 76-81 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 天野 富美夫: "EGF結合性変異株のEGF応答異常." 蛋白質・核酸・酵素.

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuge,O.: "Molecular cloning of a gene which suppresses the mutation in a phosphatidylserineーauxotroph of CHO cells." The Konrad Bloch Symposium. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuge,O.: "Molecular cloning of a gene that complements the mutation in a phosphatidylserineーauxotroph chinese hamster ovary cell mutant." 31st International Conference on the Biochemistry of lipids. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 天野 富美夫: "ヒトKB細胞由来EGFー毒素結合体耐性変異株のEGFレセプタ-異常と発癌性." 第49回日本癌学会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岩城 正昭: "トリ胚肝臓由来アフラトキシンB_1結合性蛋白質の性質およびその精製." 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 北川 隆之: "動物細胞表面膜のATPによる透過性調節と抗癌剤の作用増強." 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 天野 富美夫: "マウスマクロファ-ジ系細胞株J774.1より得られた高濃度LPS耐性変異株、LPS1916細胞における活性酸素産生異常について." 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 天野 富美夫: "マクロファ-ジ系細胞株J774.1より分離したLPS耐性変異株、LPS1916細胞におけるアラキドン酸代謝." 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 久下 小百合: "マクロファ-ジLPS受容体の遺伝生化学的解析" 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 久下 理: "ホスファチジルセリン代謝に関与するチャイニ-ズ・ハムスタ-の遺伝子とcDNAの解析" 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 花田 賢太郎: "スフィンゴ脂質生合成の欠損した突然変異動物細胞の単離と解析." 第63回日本生化学会大会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 花田 賢太郎: "スフィンゴ脂質生合成の欠損した突然変異細胞の単離と解析." 第32回日本脂質生化学研究会研究集会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 久下 小百合: "リピドAモノサッカライド類縁体のマクロファ-ジ活性化能." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 花田 賢太郎: "スフィンゴ脂質生合成の欠損した突然変異細胞の単離." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 天野 富美夫: "LPSで活性化したマクロファ-ジ系細胞株J774.1におけるプロスタグランジン遊離のシクロヘキシミドによる阻害." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田中 康仁: "マクロファ-ジ系細胞株RAW264.7の2ーアラキドニルPCまたはPEに特異的なホスホリパ-ゼA_2の分離・精製と性質." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 久下 理: "リン脂質・セリン交換酵素IのcDNAクロ-ニング." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 北川 隆之: "新規合成スルホンアミド誘導体のATP依存性動物細胞膜透過性変化の促進作用." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 岩城 正昭: "ニワトリ胚初代培養肝細胞のアフラトキシンB_1結合蛋白質." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 川越 淳一: "細胞膜透過性変化を利用したATPによる抗癌剤の増強効果." 日本薬学会第110年会. (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 益見 厚子: "Swiss 3T3細胞におけるTGFβ1による増殖と糖輸送活性の促進効果." 日本薬学会. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 花田 賢太郎: "スフィンゴミエリン合成酵素の可溶化再構成." 日本薬学会. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kuge,Osamu: "Abortive infection with Sindbis virus of a Chinese Hamster ovary cell mutant defective in phosphatidylserine and phosphatidylethanolamine" Biochim.Biophys.Acta. 986. 61-69 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tanaka,Yasuhito: "Degradation of arachidonyl phospholipids catalyzed by two phospholipases A_2 and phospholipase C in a lipopolysaccharide-treated macrophage cell line,RAW264.7" Arch.Biochem.Biophys.272. 210-218 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hasegawa,Kazuhide: "Isolation and characterization of a Chinese Hamster ovary cell mutant with altered regulation of phosphatidylserine biosynthesis" J.Biol.Chem.264. 19887-19892 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hasegawa,Kazuhide: "Isolation and characterization of chinese hamster ovary cell mutants defective in glucose transport" Biochim.Biophys.Acta. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Hara-Kuge,Sayuri: "Isolation of an LPS-resistant mutant,with defective LPS binding,of cultured macrophage-like cells" J.Biol.Chem.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 西島正弘: "マクロファ-ジ系細胞株J774.1細胞のLPS結合損傷株の分離と解析" 第63回日本細菌学会総会(徳島). (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 田中康仁: "マクロファ-ジ系細胞株RAW264.7に見い出されたCa^<2+>依存性ホスホリパ-ゼA_2の部分精製と性質" 生化学. 61. 1031 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 天野富美夫: "プロスタグランジンによるLPS活性化マクロファ-ジからのアラキドン酸遊離の調節" 生化学. 61. 934 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大賀一久世洋子: "CHOーK1細胞のイノシト-ル輸送の性質と輸送欠損変異株の分離" 日本薬学会第109年会. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 大賀一久世洋子: "CHO細胞のイノシト-ル輸送機構" 第11回生体膜と薬物の相互作用シンポジウム. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 原ー久下小百合: "マクロファ-ジ系細胞株J774.1細胞のLPS結合損傷変異株の分離と解析" 生化学. 61. 934 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 久下理: "動物細胞におけるホスファチジルセリンの生合成機構" 生化学. 61. 1259-1264 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 久下理: "リン脂質ーセリン交換酵素IのcDNAクロ-ニングの試み" 生化学. 61. 883 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 西島正弘: "毒性生化学(上)" 地人書館, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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