| Project/Area Number |
01480514
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | Teikyo University |
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Principal Investigator |
TAMURA Hiroshi Teikyo Univ. . Sch. Med., Assoc. Prof., 医学部, 助教授 (30101728)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Toshikazu Teikyo Univ. . Sch. Med., Res. Assoc., 医学部, 助手 (30082275)
DAIMON Tateo Teikyo Univ. . Sch. Med., Assoc. Prof., 医学部, 助教授 (40091037)
KIUCHI Yoshihiro Yokohama City Univ. . Sch. Med., Assoc. Prof., 医学部, 助教授 (80012764)
KUHARA Takatoshi Teikyo Univ. . Sch. Med., Assist. Prof., 医学部, 講師 (70134616)
ABE Yoshiaki Teikyo Univ. . Sch. Med., Prof., 医学部, 教授 (20089296)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Mycoplasma pulmonis / Mycoplasmal pneumonia / Interstitial pneumonia / Experimental infection / Active immunization / Passive immunization / Experimental pneumonia / Experimental animals / マイコプラズマ / マイコプラズマ プルモニス / 感染症 / ラット / 肺炎 / 免疫学 / ワクチン |
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| Research Abstract |
Using components isolated from Mycoplasma pulmonis, we prepared various lung lesions characteristic of mycoplasma-induced interstitial pneumonia in the mouse and rat to clarify the character of this form of pneumonia as a basis for the investigation of chemotherapy of mycoplasma infections and screening for a protective immunogen to M.pulmonis-induced pneumonia in mice. Production of iterstitial pneumonia by administration of non-viable components:
We were able to almost completely reproduce interstitial pneumonia in which perivascular and alveolar wall infiltration by lymphocytes can be seen in the lung from the early to maximum phase of mycoplasma infection by intranasal administration of a compound nephritogenoside, which has cross-reactive antigenicity towards the lung, and ConA, which has mitogen activity and the antigenicity of purified antigen isolated from mycoplasma culture filtrate. However, intratracheal and peribroncheal inflammations could only be induced by administration of the purified membrane of M.pulmonis. It is believed that infection-induced systemic immunological change of the host is requisite for these symptoms to develop into chronic interstitial pneumonia. Administration of antibiotics alone does not have an adequate therapeutic effect on this pneumonia. By contrast, combined administration of the immunosuppressants (prednisolone, cyclosporin A) and the immunomodulator (interleukin-2) with the antibiotic (minomycine) leads to therapeutic efficacy and studies using these drugs are still continuing to explore the mechanisms on which they act to determine an efficacious from of therapy. The experimental mouse-based infection system used in the present study for active and passive immunization of M.pulmonis-induced pneumonia in mice provided empirical evidence of the existence of antigenic components of organisms that offer significantly greater protection of mice against M.pulmonis.
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