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Production of interstitial pneumonia in mice and rats by administration of component preparations of mycoplasma, lectins and other non-viable compounds, and screening for the protective immunogen components of Mycoplsma pulmonis

Research Project

Project/Area Number 01480514
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Laboratory animal science
Research InstitutionTeikyo University

Principal Investigator

TAMURA Hiroshi  Teikyo Univ. . Sch. Med., Assoc. Prof., 医学部, 助教授 (30101728)

Co-Investigator(Kenkyū-buntansha) ISHIGURO Toshikazu  Teikyo Univ. . Sch. Med., Res. Assoc., 医学部, 助手 (30082275)
DAIMON Tateo  Teikyo Univ. . Sch. Med., Assoc. Prof., 医学部, 助教授 (40091037)
KIUCHI Yoshihiro  Yokohama City Univ. . Sch. Med., Assoc. Prof., 医学部, 助教授 (80012764)
KUHARA Takatoshi  Teikyo Univ. . Sch. Med., Assist. Prof., 医学部, 講師 (70134616)
ABE Yoshiaki  Teikyo Univ. . Sch. Med., Prof., 医学部, 教授 (20089296)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1991: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1989: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsMycoplasma pulmonis / Mycoplasmal pneumonia / Interstitial pneumonia / Experimental infection / Active immunization / Passive immunization / Experimental pneumonia / Experimental animals / マイコプラズマ / マイコプラズマ プルモニス / 感染症 / ラット / 肺炎 / 免疫学 / ワクチン
Research Abstract

Using components isolated from Mycoplasma pulmonis, we prepared various lung lesions characteristic of mycoplasma-induced interstitial pneumonia in the mouse and rat to clarify the character of this form of pneumonia as a basis for the investigation of chemotherapy of mycoplasma infections and screening for a protective immunogen to M.pulmonis-induced pneumonia in mice. Production of iterstitial pneumonia by administration of non-viable components:
We were able to almost completely reproduce interstitial pneumonia in which perivascular and alveolar wall infiltration by lymphocytes can be seen in the lung from the early to maximum phase of mycoplasma infection by intranasal administration of a compound nephritogenoside, which has cross-reactive antigenicity towards the lung, and ConA, which has mitogen activity and the antigenicity of purified antigen isolated from mycoplasma culture filtrate. However, intratracheal and peribroncheal inflammations could only be induced by administration of the purified membrane of M.pulmonis. It is believed that infection-induced systemic immunological change of the host is requisite for these symptoms to develop into chronic interstitial pneumonia. Administration of antibiotics alone does not have an adequate therapeutic effect on this pneumonia. By contrast, combined administration of the immunosuppressants (prednisolone, cyclosporin A) and the immunomodulator (interleukin-2) with the antibiotic (minomycine) leads to therapeutic efficacy and studies using these drugs are still continuing to explore the mechanisms on which they act to determine an efficacious from of therapy. The experimental mouse-based infection system used in the present study for active and passive immunization of M.pulmonis-induced pneumonia in mice provided empirical evidence of the existence of antigenic components of organisms that offer significantly greater protection of mice against M.pulmonis.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report

Research Products

(30 results)

All Other

All Publications (30 results)

  • [Publications] 田中 裕士: "マウスMycoplasma pulmonis肺炎の治療に関する検討-抗生剤とprednisoloneの投与時期について-" マイコプラズマ研究. 16. 76-79 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] DING Xiao Ding: "Differences in growth of transplantable ascites hepatoma among various lines of Donryu Rat" Experimental Animals. 38(2). 151-154 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田中 裕士: "マウスMycoplasma pulmonis肺炎における抗生剤,免疫抑制剤および免疫調整剤の治療効果に関する研究" 札幌医学雑誌. 58(6). 403-416 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田村 弘: "マウスを用いたM.pulmonis感染血清によるマイコプラズマ肺炎防御効果について" マイコプラズマ研究. 17. 69-72 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田中 裕士: "Mycoplasma肺炎における宿主細胞性免疫反応と肺病変パターン-ヒト臨床例とマウス感染実験例との対比-" マイコプラズマ研究. 19. 92-95 (1992)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] KUHARA Takatoshi: "Modulation of immune responses in mice infected experimentally with Mycoplasma pulmonis" (submitted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] TANAKA H.: "Effective chemotherapy of Mycoplasma pulmonis-induced pneumonia in mice ; treatment schedule of minomycine and prednisolone" Proc. 16th Annual Congress Jap. Soc. Mycoplasmology. 16. 76-79 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Ding X.D.: "Differences in growth of transplantable ascites hepatoma among various lines of Donryu Rat" Exp. Anim.38(2). 151-154 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] TANAKA H.: "Studies on the therapeutic efficacy of antibiotics, immunosuppressants and an immunomodulator in Mycoplasma pulmonis-induced pneumonia of mice" Sapporo Med. J.58(6). 403-416 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] TAMURA H.: "Ptotective effect of passive immunization in Mycoplasma pulmonis-induced pneumonia in mice" Proc. 17th Annual Congress Jap. Soc. Mycoplasmology. 17. 69-72 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] TANAKA H.: "Comparative pathology and delayed hypersensitivity in lungs of experimentally infected mice and patients with natural disease" Proc. 19th Annual Congress Jap. Soc. Mycoplasmology. 19. 92-95 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] KUHARA T.: "Modulation of immune responses in mice infected experimentally with Mycoplasma pulmonis"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] 田村 弘: "マイコプラズマのnot cell bound antigerの肺炎形成に果す役割について"

    • Related Report
      1991 Annual Research Report
  • [Publications] 田村 弘: "マイコプラズマ感染動物における種々の自己免疫反応と肺炎への関与"

    • Related Report
      1991 Annual Research Report
  • [Publications] 田村 弘: "マイコプラズマ肺炎における抗原・抗体複合体の役割について"

    • Related Report
      1991 Annual Research Report
  • [Publications] 田村 弘: "マイコプラズマ感染動物にみられる免疫能の低下現象の解析"

    • Related Report
      1991 Annual Research Report
  • [Publications] 田村 弘: "Mycoplasma pulmonisの生菌免疫について"

    • Related Report
      1991 Annual Research Report
  • [Publications] 田村 弘: "Mycoplasma pulmonis感染血清中の感染防御抗体の解析"

    • Related Report
      1991 Annual Research Report
  • [Publications] 田村 弘: "マウスを用いたM.pulmonis感染血清によるマイコプラズマ肺炎防御効果について" マイコプラズマ研究. 17. 69-72 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田村 弘: "マイコプラズマ肺炎の動物モデルによる解析" 第64回日本細菌学会関東支部総会抄録. 149 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田中 裕: "マウスのマイコプラズマ肺炎における宿主の非特異的細胞性免疫反応" アレルギ-. 39. 1258 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 田村 弘: "マウスを用いたT細胞非依存性マイコプラズマ肺炎に関する検討"

    • Related Report
      1990 Annual Research Report
  • [Publications] 田村 弘: "マウスのM.pulmonis肺炎に対する生菌免疫と受動免疫に関する検討"

    • Related Report
      1990 Annual Research Report
  • [Publications] 田村 弘: "M.pulmonis抗原のarrogateを用いて作成した間質性肺炎に関する検討"

    • Related Report
      1990 Annual Research Report
  • [Publications] Xia Ding DING: "Differences in growth of transplantable ascites hepatoma among various lines of Donryu rat" Experimental Animal. 38. 151-154 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 田中裕士: "マウスMycoplasma pulmonis肺炎の治療に関する検討ー抗生剤とPredonisoloneの投与時期について" マイコプラズマ学会記録. 16. 76-79 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 田中裕士: "マウスMycoplasma pulmonis肺炎における抗生剤、免疫抑制剤および免疫調節剤の治療効果に関する研究" 札幌医学雑誌. 58. (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 田村弘: "マウスを用いたTーcell非依存性マイコプラズマ肺炎に関する検討"

    • Related Report
      1989 Annual Research Report
  • [Publications] 田村弘: "生菌免疫と受動免疫によるマウスのMycoplasma pulmonis肺炎の防御効果"

    • Related Report
      1989 Annual Research Report
  • [Publications] 田村弘: "コットンラット由来マイコプラズマの特性について"

    • Related Report
      1989 Annual Research Report

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Published: 1989-03-31   Modified: 2016-04-21  

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