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Functional Structure of Centromere/Kinetochore Domain of Mammalian Chromosomes

Research Project

Project/Area Number 01480537
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionNagoya University

Principal Investigator

OKAZAKI Tuneko  Nagoya University, Department of Molecular Biology, Professor, 理学部, 教授 (10022584)

Co-Investigator(Kenkyū-buntansha) YODA Kinya  Nagoya University, Department of Molecular Biology, Research Associate, 理学部, 教務員 (30126916)
MASUMOTO Hiroshi  Nagoya University, Department of Molecular Biology, Assistant Professor, 理学部, 助手 (70229384)
MASUKATA Hisao  Nagoya University, Department of Molecular Biology, Assistant Professor, 理学部, 助手 (00199689)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥6,000,000 (Direct Cost: ¥6,000,000)
KeywordsAntigenic protein recognized by ACA / CENP-B / alphoid DNA / CENP-B box / DNA-protein interaction / centromere / kinetochore / 17塩基対モチ-フ / DNAー蛋白相互作用 / CENP-B / DNA-蛋白相互作用
Research Abstract

The purpose of this research is to obtain the basic information reguired to understand the regulation mechanism of chromosome segregation through the identification of DNA sequence and protein factors responsible for the functional structure of centromere/kinetochore of mammalian chromosomes. Following information was obtained.
1. Highly repetitive alpha-satellite DNA (alphoid DNA) is localized at centromere domain.
2. Three antigens {CENP-A (17 kD), CENP-B (80 kD) and CENP-C (140 kD)} are detected with anticentromere antibodies in sera from autoimmune patients. These antigens are specifically associated with centromere domain of human chromosomes.
3. CENP-B and alphoid DNA form a complex at the 17 bp motif (CENP-B box) in alphoid DNA.
4. CENP-B box sequences are detected at centromere domain of all human chromosomes, including Y-chromosome in which only very small amount seems to be present. The CENP-B box is also found in centromeric satellite DNA of mouse.
5. Centromere antigens which form a complex with the CENP-B box are found in nuclear extracts of mouse, Indian muntjac and monkey cells.
6. A complex formed between alphoid DNA and CENP-B consists of two DNA molecules and probably two CENP-B molecules.
Thus, CENP-B/CENP-B box interaction is widely found in mammalian chromosomes and this interaction seems to play a role in higher order formation of centromeric alphoid DNA.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Hiroshi Masumoto: "Alphoid Satellite DNA is tightly associated with centromere antigens in human chromosomes throughout the cell cysle." Exp.Cell Res.181. 181-196 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi MAsumoto: "A human centromere antigen (CENPーB) interacts with a short specific sequence in alphoid DNA,a human centromric satellite." J.Cell Biol.109. 1963-1973 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshinao Muro: "The heterogeneity of anticentromere antibodies in immunoblotting analysis." J.Rheumatology. 17. 1042-1047 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 舛本 寛: "セントロメアを構成する蛋白とDNA配列" 細胞. 21. 84-89 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 桝本 寛: "動原体" 生体の科学. 40. 543-547 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi Masumoto :"Alphoid satellite DNA is tightly associated with centromere antigens in human chromosomes throughout the cell cycle." Exp. Cell Res.181. 181-196 ((198)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Hiroshi Masumoto :"A human centromere antigen (CENP-B) interacts with a short specific sequence in alphoid DNA, a human centromeric satellite." J. Cell Biol.109. 1963-1973 ((198)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshinao Muro :"The heterogeneisy of anticentromere antibodies in immunoblotting analysis." J. Rheumatology. 17 :. 1042-1047 ((199)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshinao Muro: "The heterogeneity of anticentromere antibodies in immunoblotting analysis." J.Rheumatology. 17. 1042-1047 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Masumoto: "Alphoid satellite DNA is tightly associated with centromere antigens in human chromosomes throughout the cell cycle." Exp.Cell Res.181. 181-196 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Masumoto: "A human centromere antigen(CENP-B)interacts with a short specific sequence in alphoid DNA,a human centromeric satellite." J.Cell Biol.109. 1963-1973 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 舛本寛: "セントロメアを構成する蛋白とDNA配列" 細胞. 21. 84-89 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 舛本寛: "動原体" 生体の科学. 40. 543-547 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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