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DNA Rearrangements Caused by Non-Homologous Recombinatin and Gene Expression

Research Project

Project/Area Number 01480540
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 分子遺伝学・分子生理学
Research InstitutionScience University of Tokyo

Principal Investigator

IIDA Shigeru  Sci. Univ. Tokyo, Dept. Biol. Sci. & Tech., Professor, 基礎工学部, 教授 (30012777)

Co-Investigator(Kenkyū-buntansha) MIYAZAKI Chikara  Sci. Univ. Tokyo, Dept. Biol. Sci & Tech., Research Associate,, 基礎工学部, 助手 (40190764)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1990: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1989: ¥5,600,000 (Direct Cost: ¥5,600,000)
KeywordsGenetic Recombination / Non-Homologous Recombination / DNA Rearrangements / Gene Expression / Mobile Genetic Elements / Transposable Elements / Higher Plants / Drug Resistance Marker Genes
Research Abstract

DNA rearrangements often affect the expression of genes. Site-specific Min DNA inversion found on the plasmid p15B is a good example, which we have characterized. It consists of site-specific recombinase gene min, six crossover sites and an active recombination enhancer. Orientation of the invertible DNA segments determines the expression of sets of particular genes.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] H.Sandmeier: "Siteーspecific DNA recombination system Min of plasmid p15B: A Cluster of overlapping invertible DNA segments." proc.Natl.Asi.USA. 87. 1109-1113 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Iida: "The Min DNA ioon ppVersion enzyme of plasmid p15B of <Escheri>___ーー<chia>___ー<coli>___ー 15T^ー:A new member of the Din family of sitespecific recombinases." Molectular Microbiology. 4. 991-997 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] R.Bilang: "The 3′ーterminal region of the hygromycin B resistance gene is important for its activity in <Escherichia>___ー <coli>___ー and in <Nicotiana>___ー <tabacum>___ー." Gene.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T.Izawa: "Introduction and transposition of the maize transposable element <Ac>___ー in rice (<Oryza>___ー <sativa>___ー L.)." Mol.Gen.Genet.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Sandmeier: "Gene organization in the multiple DNA inversion region Min of plasmid p15B of E.15T: Creation of a gene with a variable 3′end." Nmcleic Acids Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S.Iida: "Expression of the downstream <ntpII>___ー gene on bicistronic transcription units in transgenic tobacco plants." Gene.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 飯田 滋(分担執筆): "シリ-ズ分子生物学の進歩13,高等植物の情報発現と制御" 丸善株式会社, 292 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 飯田 滋(分担執筆): "医薬バイオテクノロジ-事典" 株式会社広川書店, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Sandmeier: "Site-specific DNA recombination system Min of plasmid p15B : A cluster of overlapping invertible DNA segments." Proc. Natl. Acad. Sci. USA. 87. 1109-1113 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Iida: "The 3'-terminal region of the hygromycin B resistance gene is important for its activity in BEscherichia coil and in icotiana tabacum." Gene.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Izawa: "Introduction and transposition of the maize transposable element Ac in rice (Oryza sativa L.)." Mol. Gen. Genet.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H. Sandmeier: "Gene organization in the multiple DNA inversion region Min of plasmid p15B of E. coil 15T : Creation of a gene with a variable 3' end." Nucleic Acids Res.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Iida: "Expression of the down stream ntpII gene on bicistronic transcription units in transgenic tobacco plants." Gene.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Iida: Maruzen. Expression and Regulation of Genetic Information in Higher Plants., 292

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Iida: Hirokawa-publishing. Dictionary of Biotechnology in Medicine and Pharmaceutical Sciences.,

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Sandmeier: "Siteーspecific DNA recombination system Min of plasmid p15B:A cluster of overlapping invertible DNA segments." Proc.Natl.Acad.Sci.USA. 87. 1109-1113 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Iida: "The Min DNA in__ーversion enzyme of plasmid p15B of <Escherichia>___ー <coli>___ー 15T:A new member of the Din family of sitespecific recombinases." Molecular Microbiology. 4. 991-997 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] R.Bilang: "The 3'ーterminal region of the hygromycin B resistance gene is important for its activity in <Escherichia>___ー <coli>___ー and in <Nicotiana>___ー <tabacum>___ー." Gene.

    • Related Report
      1990 Annual Research Report
  • [Publications] T.Izawa: "Introduction and transposition of the maize transposable element <Ac>___ー in rice (<Oryza>___ー <sativa>___ー L.)." Mol.Gen.Genet.

    • Related Report
      1990 Annual Research Report
  • [Publications] H.Sandmeier: "Gene organization in the multiple D__ーNA inversion region Min of plasmid p15B of E__ー. <coli>___ー 15T:Creation of a gene with a variable 3' end." Nucleic Acids Res.

    • Related Report
      1990 Annual Research Report
  • [Publications] S.Iida: "Expression of the downstream <ntpII>___ー gene on bicistronic transcription units in transgenic tobacco plants." Gene.

    • Related Report
      1990 Annual Research Report
  • [Publications] 飯田 滋(分担執筆): "シリ-ズ分子生物学の進歩13.高等植物の情報発現と制御" 丸善株式会社, 292 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 飯田 滋(分担執筆): "医薬バイオテクノロジ-事典" 株式会社広川書店, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] P.Hubner: "Bent DNA is needed for recombinational enhancer activity in the site-specific recombination system Cin of bacteriophage Pl.The role of Fis protein." J.Mol.Biol.205. 493-500 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Sandmeier: "Site-specific DNA recombination system Min of plasmid p15B: A cluster of overlapping invertible DNA segments." Proc.Natl.Acad.Sci.USA. 87. 1109-1113 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Iida: "The Min DNA inversion enzyme of plasmid p15B of E.coli 15T: A new member of the Din family of site-specific recombinases." Molecular Microbiology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] H.Sandmeier: "Min DNA inversion creates a gene with a variable 3'end." Nucleic Acids Res.

    • Related Report
      1989 Annual Research Report
  • [Publications] T.Izawa: "Introduction and transposition of the maize transposable element Ac in rice(Oryza sativa L.)" Mol.Gen.Genet.

    • Related Report
      1989 Annual Research Report
  • [Publications] S.Iida: "The 3'-terminal region of the hygromycin B resistance gene is important for its activity in Escherichia coli and Nicotiana tabacum." Gene.

    • Related Report
      1989 Annual Research Report
  • [Publications] 飯田滋: "シリ-ズ分子生物学の進歩13高等植物の情報発現と制御" 丸善株式会社, 292 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 飯田滋: "医薬バイオテクノロジ-事典" 株式会社広川書店,

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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