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Development of a new Chromosome-Identification Method and Gene-Mapping of Xenopus Laevis Chromosome.

Research Project

Project/Area Number 01540529
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionGunma University

Principal Investigator

KONDO Toshihiko  Gunma Univ., Inst. Endocrinol., Assist. Prof., 内分泌研究所, 助手 (10162108)

Co-Investigator(Kenkyū-buntansha) YOSHIZATO Katutoshi  Hiroshima Univ., Fac. Sci., Professor, 理学部, 教授 (20095516)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1990: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsChromosome / Chromosome map / Gene mapping / Xenopus laevis / Sex determination mechanism / Metamorphosis / 性決定遺伝子
Research Abstract

In order to identify Xenopus chromosomes and to make a gene-map of Xenopus chromosome, we tried to develop some methods, and cloning many chromosome specific genes, hormone genes, sex specific genes (or sex determining genes), and metamorphosis (or programmed cell death) marker genes.
We developed ; 1. a highly sensitive nucleic acid detection method and gene-mapping method using chain reaction of fluorescence amplifiers, 2. a rapid isolation method for highly purified plasmid DNA, 3. a high resolution HPLC system using strong basic anion exchange resin (QA column), and 4. a DNA-immobilized anchor (DNA is enzymatically solidified by DNA ligase). By combination of these methods, we established ; 5. a new solid-phase cloning system, and 6. a hybrid selection method. According to use these method, we cloned ; 7. female specific DNAs (DNA markers of sex chromosome of Xenopus laevis, and 8. collagenase gene (a marker gene of metamorphosis in the amphibia). And, we are further cloning many chromosome specific gene markers and other marker genes. In addition to this research, we found an epidermal factor which stimulates collagenase production by fibroblasts in a reconstituted skin model, and clarified the hormonogenetic donor sites of thyroid hormone in thyroglobulin.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Ohmiya,Y.: "Separation of DNA fragments by highーresolution ionーexchange chromatography on a nonporous QA column." Anal. Biochem.189. 126-130 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohmiya,Y.: "Location of dehydroalanine residues in the amino acid sequence of bovine thyroglobulin." J. Biol. Chem.265. 9066-9071 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] ShimizuーNishikawa,K.: "An epidermal factor which stimulates collagenase production by fibroblasts in a reconstituted skin model." Biomed. Res.11. 231-241 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kondo,T.: "Rapid and efficient separation of DNA fragments." Rice Biotech. Quart.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 近藤 壽彦: "塩化リチウムと臭化エチジウムを併用する高純度プラスミドDNAの迅速分離法" 細胞工学. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kondo,T.: "Rapid isolation of plasmid DNA by LiClーethidium bromide treatment and gelーfiltration." Anal. Biochem.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 吉里 勝利: "変態の細胞生物学" 東京大学出版, (140)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 吉里 勝利: "多細胞動物への道" 東京大学出版, (215)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nishikawa, A.: "Thyroid hormone induces the keratinization and tail-specific growth inhibition of epidermal cells." Develop. Biol.131. 337-344 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohmiya, Y.: "Separation of DNA fragments by high-resolution ionexchange chromatography on a nonporous QA column." Anal. Biochem.189. 126-130 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohmiya, Y.: "Location of dehydroalanine residues in the amino acid sequence of bovine thyroglobulin." J. Biol. Chem.265. 9066-9071 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, K.: "Biochemical and immunological characterization of collagen molecules from sea urchin." Biochim. Biophys. Acta. 1038. 39-46 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takezawa, T.: "A novel technique for cell culture on a thermoresponsive polymer." Biotechnol.8. 854-856 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimizu, N. K.: "An epidermal factor which stimulates collagenase production by fibroblasts in a reconstituted skin model." Biomedical Res. 11. 231-234 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yoshizato, K.: "Biochemistry and cell biology of amphibian metamorphosis with a special emphasis on the mechanism. of removal of larval organs." Int. Rev. Cytol.119. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Asaga, H.: "Collagen gel contraction by fibroblasts requires cellular fibronection but not plasma fibronectin." Exp. Cell Res.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kondo, T.: "Rapid and efficient separation of DNA fragments." Rice Biotech. Quart.(1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kondo, T.: "Rapid isolation of plasmid DNA by LiCl-ethidium bromide treatment and gel-filtration." Anal. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ohmiya,Y.: "Separation of DNA fragments by highーresolution ionーexchange chromatography on a nonporous QA oolumn." Analytical Biochemistry. 189. 126-130 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Ohmiya,Y.: "Location of dehydroalanine residues in the amino acid sequence of bovine thyroglobulin." The Journal of Biological Chemistry. 265. 9066-9071 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] ShimizuーNishikawa,K.: "An epidermal factor which stimulates collagenase production by fibroblasts in a reconstituted skin model." Biomedical Research. 11. 231-241 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kondo,T.: "Rapid and efficient separation of DNA fragments." Rice Biotechnology Quarterly. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 近藤 壽彦: "塩化リチウムと臭化エチジウムを併用する高純度プラスミドDNAの迅速分離法" 細胞工学. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kondo,T.: "Rapid isolation of plasmid DNA by LiClーethidium bromide treatment and gelーfiltration" Analytical Biochemistry.

    • Related Report
      1990 Annual Research Report
  • [Publications] 吉里 勝利: "変態の細胞生物学(UPバイオロジ-78)" 東京大学出版, 140 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 吉里 勝利: "多細胞動物への道(UPバイオロジ-)" 東京大学出版, 215 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nishikawa,A.: "Cell death in the anuran tadpoletail:Thyroid hormone induces keratinization and tail-specific gnowth inhibition of epidermal cells" Developmental Biology. 131. 337-344 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉里勝利: "両生類幼生尾部の選択的組織分解の機構--1つの仮説--" 日本発生生物学会第22回大会発表要旨集. 150 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 近藤壽彦: "新規なDNA結合担体の開発と固相クロ-ニング法" 生化学. 61. 1128 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohmiya,Y.: "Location of dehydroalanine residues in the amino acid sequence of bovine thynoglobulin:Identifioation of“donor"tyrosine sites for honmonogenesis in thynoglo bulin." The Journal of Biological Chemistry. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Ohmiya,Y.: "Separation of DNA fragments by High-resdution Ion-exchange chromatography on a non-porous QA column." Analytical Biochemistry.

    • Related Report
      1989 Annual Research Report
  • [Publications] Kondo,T.: "Enzymatic immobilization of DNA and high performance solid-phase cloning" Science または Nucleic Acid Research.

    • Related Report
      1989 Annual Research Report
  • [Publications] Yoshizato,K.: "International Review of Cytology,vol 119" Academic Press, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 吉里勝利: "変態の細胞生物学" 東京大学出版会, 132 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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