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Studies on Mg-releasing Enzyme, Chlorophyllide-Mg-Dechelatase.

Research Project

Project/Area Number 01540566
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 植物生理学
Research InstitutionMiyazaki Medical College

Principal Investigator

SHIOI Yuzo  Miyazaki Medical College, Division of Biology, Associate professor, 医学部, 助教授 (70094092)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsChenopodium album / Chlorophyll analysis / Chlorophyll degradation / Enzymatic degradation / Mg-dechelatase / Pheophorbide accumulation / Plant pigment / Mg-releasing enzyme. / Mg-デキレタ-ゼ / Mg-脱離酵素 / フェオフォルビド
Research Abstract

The breakdown of chlorophyll (Chl) in crude extracts of Chenopodium album (white goose foot) in the dark was examined. Derivatives of pheophorbide a were formed when chlorophyll a or chlorophyllide a was incubated with depigmented crude extracts. The formation of pheophorbide was completely prevented by heat treatment of extracts, indicating that the reaction was enzymatic, and the presence of a Mg-releasing enzyme, the so called Mg-dechelatase, was postulated. This hypothesis was strongly supported by the observation that the formation of pheophorbide was inhibited by 51% by 10mM MgCl. Analysis by High-Performance Thin-Layer Chromatography (HPTLC) and liquid chromatography (HPLC) showed that the appearance of chlorophyllide a, pheophorbide'a, 13 -hydroxychlorophyllide a and pyropheophorbide a was accompanied by a concomitant decrease in levels of Chl a. It appears that Chl is degraded in a crude extract of C. album via the following enzymatically catalyzed reactions : Chl a ---- chlorophyllide a ---- pheophorbide a ---- pyropheophorbide a In the pathway of Chl breakdown, an accumulation of pheophorbide is not due to a lack of enzymes adn/or cofactors but may be explained by partial inhibition at the step distal to formation of pheophorbide derivatives which results in the accumulation of pheophorbide species. The purification and characterization of enzymes concerning the degradation of Chl are now in progress.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Shimokawa,K.: "Pyropheophorbide a,a catabolite of ethyleneーinduced chlorophyll degradation." Phytochemistry. 29. 2105-2106 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Boddi,B.: "Spectroscopic forms of divinylprotochlorophyllide in solid films." Biochim.Biophys.Acta. 1015. 116-120 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimokawa,K.: "Identification of catabolites of ethyleneーinduced chlorophyll degradation." Bull.Fac.Agric.Miyazaki Univ.37. 187-194 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shioi,Y.: "Enzymatic degradation of chlorophyll in Chenopodium album." Plant Cell Physiol.32(1). 87-93 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato,K.: "Regulation of the biosynthesis of bacteriochlorophyll. in Aerobic Photosynthetic Bacteria." Japan Scientific Society Press, 167 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shioi,Y.: "Analytical chromatography of chlorophylls.in Chlorophylls." CRC Press, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimokawa, K.: "Pyropheophorbide , a catabolite of ethylene induced chlorophyll degradation." Phytochemistry. 29(7). 2105-2106 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Boddi, B.: "Spectroscopic forms of divinylprotochlorophyllide solid films." Biochem. Biophys. Acta. 1015(1). 116-120 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimokawa, K.: "Identification of catabolites of ethylene induced chlorophyll degradation." Bull. Fac. Agric. Miyazaki Univ.37(1). 187-194 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shioi, Y.: "Enzymatic degradation of chlorophyll in Chenopodium album." Plant Cell Physiol. 32(1). (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sato, K.: "Regulation of the Biosynthesis of Bacteriochlorophyll. in Aerobic Photosynthetic Bacteria." Japan Scientific Society Press. 95-124 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shioi, Y.: "Analytical Chromatography of Chlorophylls. in Chlorophylls." CRC Press. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Shimokawa,K.: "Identification of catabolites of ethyleneーinduced chlorophyll degradation." Bull.Fac.Agric.Miyazaki. 37. 187-194 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shioi,Y.: "Enzymatic degradation of chlorophyll in Chenopodium album." Plant Cell Physiol.1号. 32. 87-93 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shioi,Y.: "Chlorophylls.Analytical chromatography of chlorophylls." CRC Press, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Shimokawa,K.: "Pheophorbide a as a catabolite of ethylene-induced chlorophyll a degradation" Phytochemistry. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Roddi,B.: "Spectroscopic forms of divinyl-protochlorophyllide in solid films" Biochim.Biophys.Acta. 1015. 116-120 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shioi,Y.: "Aerobic and anaerobic photosynthesis and bacteriochlorophyll formation in Rhodobacter sulfidophilus" Proc.International Congress on Photosynthesis. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Shioi,Y.: "Chlorophylls,Chromatography:Analytical" CRC Press, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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