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Study of the gene expression in development and regulation of methylation of DNA

Research Project

Project/Area Number 01540590
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物発生・生理学
Research InstitutionThe University of Tokyo

Principal Investigator

YASUGI Sadao  Faculty of Science, University of Tokyo Associate Professor, 理学部, 助教授 (70011591)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsregulation of gene expression / pepsinogen gene / embryonic development / methylation of DNA
Research Abstract

The aim of the present study is to elucidate the regulation mechanisms of genes which show stage-specific and organ-specific expression during animal development. I used for this purpose the embryonic chicken pepsinogen (ECPg) gene which is expressed specifically in the epithelial cells of proventriculus (glandular stomach) during chicken embryonic period.
In mouse and human, degree of methylation in DNA of pepsinogen genes has shown to become lower in the organs expressing pepsinogens. So I examined the degree of methylation in ECPg gene obtained from ECPg-expressing and ECPg-non-expressing organs.
ECPg gene showed rather wide range polymorphisms when genomic DNA from chicken organs was digested with several restriction enzymes and subjected to Southern hybridization with cDNA of ECPg as a probe. I selected, therefore, the individuals which had the same hybridization pattern for further analysis. DNAs from proventriculus, gizzard, small intestine and muscle showed the same hybridization pattern when they were digested with methylation-sensitive restriction endonucleases such as Hha I or Hpa II. These results indicate that, ECPg expresssion is not regulated by methylation of DNA itself, but by sis- or trans-acting factors in the proventricular epithelial cells.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] YASUGI, S.: "Pepsinogenーlike immunoreactivity in ascidian stomach and intestine: Immunohistochemical and biochemical study." Zool. Sci.6. 283-288 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YASUGI, S. et al.: "Differentiation of proventricular epithelium in xenoplastic associations with mesenchymal or fibroblastic cells." Roux' Arch. Develop. Biol.198. 114-117 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YASUGI, S.: "Mesenchymalーepithelial interactions in the organogenesis of digestive tract." Zool. Sci.7. 159-170 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] TAKIGUCHIーHAYASHI, K.: "Transfilter analysis of the inductive influence of proventricular mesenchyme on stomach epithelial differentiation of chick embryos." Roux' ArcH. Develop. Biol.198. 460-466 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] MIZUNO, T.: "Susceptibility of epithelia to directive influence of mesenchymes during organogenesis: Uncoupling of morphogenesis and cytodifferentiation." Cell Differ. Develop.31. 151-159 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] TAKIGUCHIーHAYASHI, K.: "Localized distribution of a novel mesenchymeーepecific antigen in developing chick digestive organs: Comparison with the distribution of fibronectin, laminin and tenascin." Roux' Arch, Develop. Biol.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasugi, S. et al.: "Pepsinogen-like immunoreactivity in ascidian stomach and intestine : Immunohistochemical and biochemical study." Zool. Sci.6. 283-288 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasugi, S. et al.: "Differentiation of proventricular epithelium in xenoplastic associations with mesenchymal or fibroblastic cells." Roux' Arch. Dev. Biol.198. 114-117 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasugi, S. et al.: "Mesenchymal-epithelial interactions in the organogenesis of digestive tract" Zool. Sci.7. 159-170 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takiguchi-Hayashi, K. et al.: "Transfilter analysis of the inductile influence of proventricular mesenchyme on stomach epithelial differentiation of chick embryos." Roux' Arch. Dev. Biol.198. 460-466 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Mizuno, T. et al.: "Susceptibility of epithelia to directive influence of mesenchymes during organogenesis : Uncoupling of morphogenesis and cytodifferentiation." Cell Differ. Dev.31. 151-159 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Takiguchi-Hayashi, K. et al.: "Localized distribution of a novel mesenchyme-specific antigen in developing chick digestive organs : Comparison with the distribution of fibronectin, laminin and tenascin." Roux' Arch. Dev. Biol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yasugi, S. et al.: "Early determination of developmental fate in presumptive intestinal endoderm of the chicken embryo." Dev. Growth and Differ.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] YASUGI,S.: "Pepsinogenーlike immunoreactivity in ascidian stomach and intestine:Immunohistochemical and biochemical study." Zool.Sci.6. 283-288 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] YASUGI,S.et al.: "Differentiation of proventricular epithelium in xenoplastic associations with mesenchymal or fibroblastic cells." Roux'Arch.Develop.Biol.198. 114-117 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] YASUGI,S.: "Mesenchymalーepithelial interactions in the organogenesis of digestive tract." Zool.Sci.7. 159-170 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] TAKIGUCHIーHAYASHI,K.: "Transfilter analysis of the inductive influence of proventricular mesenchyme on stomach epithelial differentiation of chick embryos." Roux' Arch.Develop.Biol.198. 460-466 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] MIZUNO,T.: "Susceptibility of epithelia to directive influence of mesenchymes during organogenesis:Uncoupling of morphogenesis and cytodifferentiation." Cell Differ.Develop.31. 151-159 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] TAKIGUCHIーHAYASHI,K.: "Localized distribution of a novel mesenchymeーepecific antigen in developing chick digestive organs:Comparison with the distribution of fibronectin,laminin and tenascin." Roux' Arch.Develop.Biol.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] YASUGI,S.: "Pepsinogen-like immunoreactivity in ascidian stomach and intestine:Immunohistochemical and biochemical study." Zool.Sci.6. 283-288 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] YASUGI,S.: "Differentiation of proventricular epithelium in xenoplastic associations with mesenchymal or fibroblastic cells." Roux'Arch.Develop.Biol.198. 114-117 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 八杉貞雄: "組織間情報メディエ-タ-と細胞分化" 実験医学. 7. 1169-1176 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] YASUGI,S.: "Mesenchymal-epithelial interactions in the organogenesis of digestive tract." Zool.Sci.6. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] TAKIGUCHI-HAYASHI,K.: "Transfilter analysis of the inductive influence of proventricular mesenchyme on stomach epithelial differentiation of chick embryos." Roux'Arch Develop.Biol.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] TAKIGUCHI-HAYASHI,K.: "Regulation of keratine expression in the digestive tract epithelia by the proventricular mesenchyme of chick embryos in heterologous epithelial-mesenchymal recombinations." Development. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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