| Project/Area Number |
01540591
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMADA Kazumi (1990) Hiroshima University Faculty of Science, Instructor, 理学部, 助手 (80220367)
赤坂 甲治 (1989) 広島大学, 理学部動物学教室, 助教授 (60150968)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1990: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Sea urchin / Development / Arylsulfatase / Gene cloning / In situ hybridization / Activity staining / DNA synthesis / Gastrulation / ウニ胚 / 活性染色 / 分子発生学 |
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| Research Abstract |
In development of sea urchin embryos, the arylsulfatase activity begins to make a drastic increase at the mesenchyme blastula stage prior to the onset of gastrulation. The present research project has been undertaken to elucidate roles of arylsulfatase in the process of gastrulation. The following are the result of the two-years research project.
(1) The arylsulfatase (Ars) gene of the embryo of the sea urchin, Hemicentrotus pulcherrimus, was cloned and its sequence was determined.
(2) By in situ hybridization using anti-sense RNA of Ars-cDNA as the probe, it was found that the Ars gene is transcribed only in aboral ectoderm of sea urchin gastrula.
(3) By the activity staining of arylsulfatase with optical as well as electron microscopes, it was found that arylulfatase localizes in lysosomes of qastrula, prism and pluteus larvae. It is suggested that arylsulfatase supplies sulfate ions through hydrolysis of yolk material which are needed to support gastrulation.
(4) Addition of aphidicolin, an inhibitor of DNA synthesis, to sea urchin embryos resulted in the prevention of transcription of the Ars gene. The result was interpreted as that timing of the Ars gene transcription is somehow related to cycles of DNA replication after fertilization.
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