• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Studies on the avian Mullerian inhibiting substance (MIS) and its receptor.

Research Project

Project/Area Number 01540612
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 動物形態・分類学
Research InstitutionSaitama University

Principal Investigator

NOUMURA Tetsuo  Saitama Univ. Fac. of Science, Professor, 理学部, 教授 (40072970)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Tetsuya  Saitama Univ., Fac. of Science, Lecturer, 理学部, 講師 (00195794)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsMullerian inhibiting substance / Estrogen / Testis / Mullerian duct / chick / quail / Receptor / organ culture / ミユラ-管 / 鳥類 / ミュラ-管抑制物質 / mRNA
Research Abstract

1. Purification of avian Mu llerian inhibiting substance (MIS) was performed from the secretory proteins in incubation medium of newly-hatched or 8-week-old chicken testes using ion exchange column chromatographic procedures. The proteins obtained in various steps during column chromatography were separated by SDS/polyacrylamide gel electrophoresis. Mullerian inhibiting activity was assayed by a graded organ-culture method using the highly susceptible mullerian ducts of a 7-day female quail embryo.
2. Exogenous synthetic estrogen, diethylstilbestrol (DES) treatment at 3 days of embryonic development prevents regression of male Mullerian ducts as well as causes feminization of male gonads in the quail. The mechanism of the DES prevention of Mullerian duct regression from MIS was studied. Testes from 8-day-old embryo and Mullerian ducts from 7-day-old embryo were cocultured in combination of normal to normal, normal to DES-treated, and DES-treated to DES-treated, in culture medium with or without DES. The results suggest that estrogen prevents Mullerian duct regression directly as well as inhibits MIS secretion from the feminized gonad.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi