| Project/Area Number |
01550579
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
工業分析化学
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| Research Institution | Nagasaki University |
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Principal Investigator |
NAKASHIMA Naotoshi Nagasaki Univ. Industrial Chem. Associate Professor, 工学部, 助教授 (80136530)
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| Co-Investigator(Kenkyū-buntansha) |
KUNITAKE Masashi Nagasaki Univ. Graduate school of Marine Science and engineering Research Associ, 大学院・海洋生産科学研究科, 助手 (40205109)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Bilayer Membrane / Bilayer Electrode / Monolayer Electrode / Functional Electrode / Impedance / Cyclic Voltammogram / Phase Transition / Bilayer Sensor / 情報変換 / 分子認識 / 蛍光プロ-ブ / クラウンエ-テル / 蛍光性クラウンエ-テル |
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| Research Abstract |
Biological membranes are composed of lipid bilayer and membrane proteins and act as transducers which convert chemical signals to electrical signals. The aim/purpose of this study is to develop novel molecular recognition and information transduction systems mimic to biological membranes. The summaries are as follows.
(1) A mercaptan-containing phosphate lipid was designed and prepared. Monolayer of the lipid was fabricated on gold electrodes via chemisorption from ethanol and their properties were examined by cyclic voltametry. The permeation or diffusion of ferricyanide and ferrocene ammonium into the monolayer on the electrode could be controlled by changing pH of the solution. The electrodes were found to response to alkali earth metal ions.
(2) Fundamental examinations on design and fabrication of bilayer devices which transduce molecular information to electric or electrochemical signals were conducted. Quaternary ammonium lipids on patterned electrodes showed drastic change in impedance and capacitance upon heating above phase transition of bilayers. However, primary ammonium lipids exhibited no such phase transition dependence. Despite the very small transition enthalpy, single chain quaternary ammonium film showed the same Tc dependence in impedance measurements as those with double chain lipids. Impedance characteristics of biological lipid films were also investigated. Molecular mechanisms for the obtained results were analyzed by cole-cole plot analyses of impedance.
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