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Immunoassay with High Sensitivity by Using Immunoliposomes and Membrane Lysis Reaction of the Complement System

Research Project

Project/Area Number 01550758
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 反応工学
Research InstitutionKyoto University

Principal Investigator

KATOH Shigeo  Kyoto Univ., Faculty of Eng., Lecturer, 工学部, 講師 (20026272)

Co-Investigator(Kenkyū-buntansha) TERASHIMA Masaaki  Kyoto Univ., Faculty of Eng., Instructor, 工学部, 助手 (30172092)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1990: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsImmunoliposome / Antigen-antibody complex / Complement system / Immunoassay / Measurement of complement activity / 抗体抗原複合体 / 高感度分析
Research Abstract

Immunoassays, which depend on the high affinity and sensitivity between antigen and antibody, are widely used in microanalyses and clinical assays. The present solid-phase immunoassays, however, need separation between bound and free antibodies and so are time-consuming and troublesome. In the present work a new homogeneous immunoassay by using immunoliposomes, i. e. liposomes coupled with antibodies (antigens), was developed. In this method, these liposomes are destroyed by the lysis reaction of the complement system on the formation of antigen-antibody complexes, and the released amount of liposome-encapsulated marker serves as a quantitative indication of the reaction taking place. The results are summarized as follows :
1. The immunoliposomes were stable in buffer solutions containing gelatin for several months without any loss of encapsulated marker.
2. The immunoliposomes coupled with rabbit IgG were destroyed mainly by the alternative pathway of complement. Addition of anti-rabbit IgG antibody enhanced significantly marker release because of the activation of the classical pathway of complement. The activities of both the pathways can be measured in the same system by using the immunoliposomes.
3. The immunoliposomes coupled with an antigen were destroyed by addition of the specific antibody against the antigen. The marker release was proportional to the amount of antigen-antibody complexes formed on the surface of the immunoliposomes.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Masaaki Kishimura: "A Simple Method for Measuring the Complement Activities of Both Classical and Alternative Pathways by Using Rabbit γーGlobulinーCoupled Liposomes" Journal of Fermentation and Bioengineering. 68. 395-398 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masaaki Kishimura: "A Simple Method for Measuring the Complement Activities of Both Classical and Alternative Pathways by Using Rabbit *-Globulin-Coupled Liposomes" Journal of Fermentation and Bioengineering. Vol. 68. 395-398 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Masaaki Kishimura: "A Simple Method for Measuring the Complement Activities of Both Classical and Alternative Pathways by Using Rabbit γーGlobulinーCoupled Liposomes" Journal of Fermentation and Bioengineering. 68. 395-398 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Masaaki Kishimura: "A Simple Method for Measuring the Complement Activities of Both Classical and Alternative Pathways by Using Rabbit γ-Globlin-Coupled Liposomes" Journal of Fermentation and Bioengineering. 68. 395-398 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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