| Project/Area Number |
01560011
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | The Japan Kinoko Research Center Foundation |
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Principal Investigator |
ARITA Ikuo The Japan Kinoko Res. Ctr. Foundation, The Tottori Mycological Institution, Chief of Section, 菌蕈研究所, 部長 (50088845)
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| Co-Investigator(Kenkyū-buntansha) |
HASEBE Kozaburo The Japan Kinoko Res. Ctr. Foundation, The Tottori Mycological Institution, Rese, 菌蕈研究所, 室長代理 (10124330)
OHIRA Ikuo The Japan Kinoko Res. Ctr. Foundation, The Tottori Mycological Institution, Head, 菌蕈研究所, 室長 (10088841)
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| Project Period (FY) |
1989 – 1991
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| Project Status |
Completed (Fiscal Year 1991)
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| Budget Amount *help |
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1991: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1990: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Lentinus edodes / Basidiomycete / fruiting-time type / genetic analysis / marker gene / linkage / 連鎖 |
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| Research Abstract |
The fruiting period of dikaryotic strains of Shiitake(Lentinus edodes)grown on wood logs under natural climatic conditions was divided into three types : (1)H(high-temperature)type in which fruiting occurred from June to August ; (2)M(medium-temperature)type, fruiting from September to next April ; and(3)L(low-temperature)type, fruiting from December to next April. The fruiting-time types on logs and fruiting on sawdust medium at 20 ﾟC were analyzed genetically using F, dikaryons derived from diallel crosses among seventeen homokaryons and progeny of some crosses.
(1)Genetic control of fruiting at a high temperature(20 ﾟC)on sawdust medium : F, dikaryons and progeny of three crosses were examined in their ability to fruit at 20 ﾟC on sawdust medium. The fruiting at 20 ﾟC appeared to be controlled by two distinct genetic systems ; one. being dominant single gene and the other consisting of dominant complementary genes. Linkage analysis suggested that one of the complementary genes might be loosely linked to the marker gene, mor-2 or mor-4.
(2)Genetic control of fruiting-time type on logs : F_1 dikaryons and progeny of a cross were analyzed for their fruiting-time types on logs under natural climatic conditions. The F_1 analysis indicated that the H-type fruiting was dominant over the other two types, and that the M type was over the L type. All the crosses between basidiospore progeny of F_1 dikaryon(L type)and a tester homokaryon exhibited the L-type fruiting, suggesting that the L type was expressed by homozygotes with identical recessive allele at the locus(loci)controlling the fruiting-time type.
All of the dikaryons that exhibited the H type on logs fruited on sawdust medium at 20ﾟC, and thus the sawdust method can be used for screening tests of the H-type strains.
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