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Basic Research for Development of Biocatalyst-modified Electrodes

Research Project

Project/Area Number 01560150
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 製造化学・食品
Research InstitutionKyoto University

Principal Investigator

IKEDA Tokuji  Kyoto Univ. Dep. Agriculture Associate Professor, 農学部, 助教授 (40026422)

Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsBiocatalyst Electrode / Enzyme electrode / Bioelectrocatalysis / Bioreactor / Biosensor / Biocatalyst / Electron Transfer / Modified Electrode / 酵素固定化電極 / 電気化学リアクタ / エレクトロカタリシス / 接触電解酸化 / 酸化還元酵素 / NAD@NADH電解 / 細胞膜タンパク質
Research Abstract

This study was carried out for aiming at the development of enzyme-modified electrodes functioning as biocatalyst electrodes which are applicable to a wide variety of field such as those in biosensor and bioreactor technologies. Two kinds of enzymes, ie. diaphorase and membrane-bound enzymes were used. Diaphoraso was immobilized on the surface of a carbon paste electrode by the method developed by the authos. The electrode functioned as an exellent NADH sensor. A combination of the diaphorase reaction with a dehydrogenase reaction allowed for the detection of NADH and NAD as low as picomolar order, based on a cycling effect. A quantitative method has been establised for evaluating the capability of a variety of redox compounds for electron transfer mediation between the enzyme and the electrode. A quinoprotein and flavoprotein, both of which contain heme C in their molecules, were examined as candidates suitable for immobilizing on the surface of electrodes of a variety of materials. They were found to be adsorbed on these electrode surface keeping with their enzyme activity and allowing the direct electron transfer at the electrodes. Thus we have found a suitable system for constructiang the biocat alyst electrode. Some applications of this type of electrode were also studied ; for example, a possibility for microtype fructose sensor based on the direct electron transfer between a carbon paste electrode and fructose dehydrogenase adsorbed on it was suggested based on the present study.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] 池田 篤治: "キノプロテインー及びフラボプロテイン・デヒドロゲナ-ゼを用いるアンペロメトリックバイオセンサ-.定常電流測定法と高感度ボルタンメトリ-法" 分析化学. 38. 583-588 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tokuji IKeda: "Amperometric response to reducing carbohydrates of an enzyme electrode based on oligosaccharide dehydiogenase.Detection of lactose and α‐amylase" Analytica Chimica Acta. 230. 75-82 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tokuji Ikeda: "DーFructose dehydrogenaseーmodified carbon paste electrode containing pーbenzoquinone as a mediated amperometic Fructose seneei" Agric,Biol,Chem.,. 54. 2919-2924 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Setsuko Todoriki: "The Chemically Amplified Response of a MembraneーCovered Diaphorase and Glucoseー6ーphosphate dehydrogenase Coimmobilized carbon paste electrode to NADH and NAD" DENKI KAGAKU. 58. 1089-1096 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tokuji Ikeda: "Ampeeometric Fructose Sensor Based on Direct Bioelectrocatalysis" Biosensors & Bioelectronics. 6. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tokuji Ikeda: "Nucleoside Oxidareーmodified Carbon Paste Electrode containing Quinane.Cathodic Current Response to Nucleosides" Electro analysis.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ikeda, F. Fushimi, K. Miki and M. Senda: "Amperometric Biosensors Based on Quinoprotein-, Flavoprotein-Dehydrogenases ; Methods of Stetdy-State Current Measurements and Voltammetric Measurements at Higher Sensitivity." Bunseki Kagaku. 38. 583-8 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ikeda, T. Shibata, S. Todoriki, M. Senda and H. Kinoshita: "Amperometric Response to Reducing Carbohydrates of an Enzyme Electrode Based on Oligosaccharide Dehydrogenase. Detection of Lactose and -Amylase." Analytica Chimica Acta. 230. 75-82 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ikeda, F. Matsushita and M. Senda: "D-Fructose-Dehydrogenase-Modified Carbon Paste Electrode Containing P-Benzoquinone as a Mediated Amperometric Fructose Sensor." Agric. Biol. Chem.54. 2919-24 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Ozawa, T. Ikeda and M. Senda: "Electrocatalytic Oxidation of NADH Basede on Diaphorase Catalysis." Rev. Polarogr (Kyoto. 36. 38 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Miyaoka, F. Matsushita, T. Ikeda and M. Senda: "Bioelectrocatalysis at Enzyme-Modified Electrodes. Effect of Chemical Modification of the Mercury Electrode Surface." Rev. Polarogr (Kyoto). 36. 39 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Miyaoka, F. Matsushita, T. Ikeda and M. Senda: "Bioelectrocatalysis at Enzyme-Modified Electrodes. Effect of Various Electrode Materials." Rev. Polarogr (Kyoto). 36. 37 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ikeda, K. Isono and M. Senda: "Nucleoside Oxidase Electrode. Cathodic Current Response Due to the Oxidation of Nucleosides." Rev. Polarogr (Kyoto). 36. 36 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] S. Todoriki, K. Miki, T. Ikeda and M. Senda: "The Chemically Amplified Response of a Membrane-Covered Diaphorase and Glucose-6-Phosphate Dehydrogenase Co-Immobilized Carbon Paste Electrode to NADH." Denki Kagaku. 58. 1089-1096 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] T. Ikeda, F. Matsushita and M. Senda: "Amperometric Fructose Sensor Based on Direct Bioelectrocatalysis." Biosensors & Bioelectronics. 6. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tokuji Ikeda: "DーFructose Dehydrogenaseーmodifid Carbon Paste Electrode Containing δーBenzoquinone as a Mediated Amperometric Fructose Sensor" Agric.Biol.Chem.54. 2919-2924 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Setsuko Todoriki: "The Chemically Amplified Response of a MembraneーCovered Diaphorase and Glucoseー6ーPhosphate Dehydrogenase CoーimmoLiliqed Carbon Paste Electrode to NADH and NAD" DENKI KAGAKU. 58. 1089-1096 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tokuji IKeda: "Amperometric fructose sensor based on direct bioelectrocatalysis" Biosensors & Bioelectsonics. 6. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tokuji Ikeda: "Nucleoside Oxidaseーmodified casbon Paste Electrode Containing Ouinone.Cathodic cusrent response to Nucleosides" Electro analysis.

    • Related Report
      1990 Annual Research Report
  • [Publications] Tokuji Ikeda: "Direct Electron Tiansfer Between and Electrode at Glseconate Dehydrogenuse Immobilized Electrodes." J.Electro analytical chem,.

    • Related Report
      1990 Annual Research Report
  • [Publications] Tokuji Ikeda: "Electrocatalytic Oxidution of Ethanol at Alcohol Dehydrojenaseーmodified Electrodes" Agric.Biol.Chem.

    • Related Report
      1990 Annual Research Report
  • [Publications] 池田篤治: "キャプロテイン-及びフラボプロテイン-デヒドロゲナ-ゼを用いるアンペロメトリックバイオセンサ-" 分析化学. 38. 583-588 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 池田篤治: "Amperometric response to reducing carbohydrates of an enzyme electrode based on oligosaccharide dehydrogenase." Analytica Chimica Acta. (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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