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Biochemical Studies on Smoltification of Salmon

Research Project

Project/Area Number 01560215
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionHokkaido University

Principal Investigator

HARA Akihiko  Hokkaido University, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (40091483)

Co-Investigator(Kenkyū-buntansha) YAMAUCHI Kohei  Hokkaido University, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (10109514)
SANEYOSHI Mineo  Hokkaido University, Faculty of Pharmaceutical Sciences, Associate Professor (pr, 薬学部(現:西東京科学大学・理工学部), 助教授(教授) (20002339)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1993)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsSalmonids / Serum protein / Electrophoresis / Guanine-binding protein / Purine nucleoside phosphorylase / Thyroid hormone / Smoltification / Parr-smolt transformation / プリンヌクレオシドホスホリラ-ゼ
Research Abstract

Young anadoromous salmonids are known to migrate from the river to the sea after smoltification (parr-smolt transformation). Smoltification is a complex phenomenon comprised of various morphological, physiological and behavioral changes. In the body13EA\ : 'silvering' phenomenon, guanine is accumulated in the skin under regulation by thyroid hormone. This research is relevant to our basic understanding of the parr-smolt transformation from the point-of-view of biochemical mechanisms.
We analyzed serum proteins during parr-smolt transformation (May-June) of masu salmon (Oncorhynchus masou) by two-dimensional polyacrylamide gel electrophoresis and Western blotting using a rabbit antiserum raised against smolt serum. A new protein (mol. wt. 43,000-50,000, p1 5.0-5.5) appeared in serum of smolt but not parr. This protein seems to be a smolt-specific serum protein. Four guanine-binding proteins were observed in parr or smolt serum by immunoelectrophoresis and autoradiography using radioactive guanine. The smolt-specific protein and guanine-binding protein may be related.
The correlation between purine nucleoside phosphorylase (PNPase) activity in liver of steelhead trout, Oncorhynchus mykiss, and smoltification stages was studied. It was found that changes of purine nucleoside phosphorylase activity in the liver wer13EA\ : e closely associated with the accumulation of guanine in the skin of steelhead trout. Two separated peaks of activity were found. The first peak occured during March to April and the second peak was seen in late May, which was corresponded to the fi13EA\ : rst accumulation of guanine and the restoration of guanine caused by descalation, respectively. These results strongly suggest that the liberation of guanine in liver was correleated to phenotype of parr-smolt transformation in anadromous steelhead trout.

Report

(3 results)
  • 1993 Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Noboru KASAHARA: "Physiological and behavioral changes in the accelerated underyearling broodstock masu salmon,Oncorhynchus masou,during smoltification." Aquaculture. 82. 21-28 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohei YAMAUCHI: "Osmoregulation and circulating growth hormone and prolactin levels in hypophysectmized coho salmon (Oncorhynchus kisutch) after trnasfer to fresh water and sea water." Aquaculture. 92. 33-42 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki YAMADA: "Serum thyroxine,estradiol-17beta and testosterone profiles accompanying smoltification in the masu salmon,Oncorhynchus masou." Fish.Physiol.Biochem. (投稿中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki YAMADA: "Changes in thyroid hormone binding sites and thyroxine deiodination in hepatocyte of the masu salmon,Oncorhynchus masou,during smoltification." (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki YAMADA: "Possible involvement of sex steroids and ovine growth hormone in thyroxine deiodination in several tissues of the yearling masu salmon,Oncorhynchus masou." (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noboru KASAHARA: "Physiological and behavioral changes in the accelerated underyealing broodstock masu salmon, Oncorhynchus masou, during smoltification." Aquaculture. 82. 21-28 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Kohei YAMAUCHI: "Osmoregulation and circulating growth hormone and prolactin levels in hypophysectmized coho salmon (Oncorhynchus kisutch) after transfer to fresh water and sea water." Aquaculture. 92. 33-42 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki YAMADA: "Serum thyroxine, estradiol-17bata and testosterone profiles accompanyling smoltification in the masu salmon, Oncorhynchus masou." Fish. Physiol. Biochem.(in submitted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki YAMADA: "Changes in throid hormone binding sites and thyroxine deiodination in hepatocyte of the masu salmon, Oncorhynchus masou, during smoltification." in preparation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Hideaki YAMADA: "Possible involvement of sex steroids and ovine growth hormone in thyroxine deiodination in several tissues of the yearling masu salmon, Oncorhynchus masou." in preparation.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1993 Final Research Report Summary
  • [Publications] Noboru KASAHARA: "Physiological and behavioral changes in the accelerated underyearling broodstock masu salmon,Oncorhynchus masou,during smoltification." Aquaculture. 82. 21-28 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kohei YAMAUCHI: "Osmoregulation and circulating growth hormone and prolactin levels in hypophysectmized coho salmon (Oncorhynchus kisutch) after transfer to fresh water and sea water." Aquaculture. 92. 33-42 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideaki YAMADA: "Serum thyroxine,estradiolー17β and testosterone profiles accompanying smoltification in the masu salmon,Oncorhynchus masou" Fish.Physiol.Biochem.

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideaki YAMADA: "Changes in thyroid hormone binding sites and thyroxine deiodination in hepatocyte of the masu salmon,Oncorhynchus masou,during smoltification."

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideaki YAMADA: "Possible involvement of sex steroids and ovine growth hormone in thyroxine deiodination in several tissues of the yearling masu salmon,Oncorhynchus masou."

    • Related Report
      1990 Annual Research Report
  • [Publications] Hideaki YAMADA: "Changes in thyroid hormone binding sites and thyroxine deiodination in hepatocyte of the masu salmon,Oncorhynchus masou,during smoltification." J.Exp.Zool.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideaki YAMADA: "Thyroxine,estradiol-17β and testosterone profiles in the masu salmon,Oncorhynchus masou,during smoltification." Gen.Comp.Endocrinol.

    • Related Report
      1989 Annual Research Report
  • [Publications] Hideaki YAMADA: "Possible involvement of sex steroids and ovine growth hormone in thyroxine deiodination in several tissues of the yearling masu salmon,Oncorhynchus masou." J.Exp.Zool.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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