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Applied Studies on Lytic Enzyme-Producing Microorganisms

Research Project

Project/Area Number 01560223
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Fisheries chemistry
Research InstitutionMie University

Principal Investigator

SUGAHARA Isao  Mie Univ., Fac. Bioresources Professor, 生物資源学部, 教授 (80024826)

Co-Investigator(Kenkyū-buntansha) KIMURA Toshio  Mie Univ., Fac. Bioresources Assistant Researcher, 生物資源学部, 助手 (50024831)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsLytic enzyme / Myco-lytic microorganisms / Utilization of lytic enzyme / Marine bacteria / Chitinolytic bacteria / 低温性溶菌酵素生産菌 / キチン分解菌 / 微生物細胞溶解スペクトル / キチン分解能 / キチン脱アセチル化能
Research Abstract

Microorganisms capable of lysing the bacterial cells at 5^゚C Were infrequent in marine environments, compared to those capable of lysing the cells at 20-25^゚C. Out of 33 strains of lytic enzyme-producing bacteria isolated, Bacillus (81.8%) Was the most dominant bacterial genus. seudomonas and Vibrio comprised 18.2% of the bacteriolytic bacteria isolated. The optimum temperature for production of lytic enzyme of the representative strains Bacillus sp. T301,T303 and E33 was around 13-17^゚C. The optimum temperature for lytic activity of strains T301, T303 and E33 was around 30-40^゚C.
Myco-lytic microorganism strain No.5 was isolated from mangrove swamp. Strain No.5 was found to produce myco-lytic enzyme when aerobically grown at 30^゚C in 90% seawater medium containing polypepton (0.5%) and vyast extract (0.3%). The optimum PH and temperature for lytic activity of strain No. 5 were around 9 and 50-60^゚C, respectively. Lytic activity was completely inactivated at 70^゚C and 10 min. Lytic activity was inhibited by 0.05-0.20 M of inorganic salts such as NaC1, KC1, Mgcl_2, Cacl_2,_2, and 0.1 mM of protease inhibitors such as leupeptin, apain, phosphoramidon and E 64.
An attempt to increase in myco-lysis using chitin-decomposing bacteria isolated was made. Lytic activity was enhanced about 1.5 times by the combination of strain No.5 enzyme and chitinolytic strain F190 enzyme.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] 菅原 庸: "海洋環境における溶菌酵素生産菌" マリンバイオテクノロジ-研究会報. 3. 3-15 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 菅原 庸: "海洋環境における溶菌酵素生産菌生態学的役割は何か" 化学と生物. 28. 771-773 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 菅原 庸・大村 俊夫・井上 洋美・大石 琢哉: "海洋環境から分離した真菌細胞溶解微生物(英文)" 日本水産学誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 菅原 庸・木村 俊夫: "海洋環境から分離した溶菌酵素生産菌の生育および溶菌活性に及ぼす低温の影響(英文)" 日本水産学会誌.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 清水 潮(編),菅原 庸: "海洋微生物とその活性「溶菌・抗菌能を有する微生物」を執筆" 技報堂出版, 350 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Isao SUGAHARA: "Lytic Enzyme-producing Bacteria in Marine Environments." J. Marine Biotechnology. vol. 3, No. 1. 3-15 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Isao SUGAHARA: "Lytic Enzyme-Producing Bacteria in Marine Environments Their Ecological Role." Kagaku to Seibutsu. vol. 28, No. 12. 771-773 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 菅原 庸: "海洋環境における溶菌酵素生産菌" マリンバイオテクノロジ-研究会報. 3. 3-15 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅原 庸: "海洋環境における溶菌酵素生産菌 生態学的役割は何か" 化学と生物. 28. 771-773 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅原 庸・木村 俊夫・井上 洋美・大石 琢哉: "海洋環境から分離した真菌細胞溶解微生物(英文)" 日本水産会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅原 庸・木村 俊夫: "海洋環境から分離した溶菌酵素生産菌の生育および溶菌活性に及ぼす低温の影響(英文)" 日本水産会誌.

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅原 庸 「溶菌・抗菌能を有する微生物」を執筆: "海洋微生物とその活性 清水潮(編)" 技報堂出版, 350 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 菅原庸: "海洋環境から分離した真菌(カビ・酵母)細胞溶解微生物(英文)" 日本水産学会誌.

    • Related Report
      1989 Annual Research Report
  • [Publications] 菅原庸: "海洋環境から分離した溶菌酵素生産菌の生育および溶菌活性に及ぼす低温の影響(英文)" 日本水産学会誌.

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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