Applied Studies on Lytic Enzyme-Producing Microorganisms
| Project/Area Number |
01560223
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Fisheries chemistry
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| Research Institution | Mie University |
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Principal Investigator |
SUGAHARA Isao Mie Univ., Fac. Bioresources Professor, 生物資源学部, 教授 (80024826)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Toshio Mie Univ., Fac. Bioresources Assistant Researcher, 生物資源学部, 助手 (50024831)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1989: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Lytic enzyme / Myco-lytic microorganisms / Utilization of lytic enzyme / Marine bacteria / Chitinolytic bacteria / 低温性溶菌酵素生産菌 / キチン分解菌 / 微生物細胞溶解スペクトル / キチン分解能 / キチン脱アセチル化能 |
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| Research Abstract |
Microorganisms capable of lysing the bacterial cells at 5^ﾟC Were infrequent in marine environments, compared to those capable of lysing the cells at 20-25^ﾟC. Out of 33 strains of lytic enzyme-producing bacteria isolated, Bacillus (81.8%) Was the most dominant bacterial genus. seudomonas and Vibrio comprised 18.2% of the bacteriolytic bacteria isolated. The optimum temperature for production of lytic enzyme of the representative strains Bacillus sp. T301,T303 and E33 was around 13-17^ﾟC. The optimum temperature for lytic activity of strains T301, T303 and E33 was around 30-40^ﾟC.
Myco-lytic microorganism strain No.5 was isolated from mangrove swamp. Strain No.5 was found to produce myco-lytic enzyme when aerobically grown at 30^ﾟC in 90% seawater medium containing polypepton (0.5%) and vyast extract (0.3%). The optimum PH and temperature for lytic activity of strain No. 5 were around 9 and 50-60^ﾟC, respectively. Lytic activity was completely inactivated at 70^ﾟC and 10 min. Lytic activity was inhibited by 0.05-0.20 M of inorganic salts such as NaC1, KC1, Mgcl_2, Cacl_2,_2, and 0.1 mM of protease inhibitors such as leupeptin, apain, phosphoramidon and E 64.
An attempt to increase in myco-lysis using chitin-decomposing bacteria isolated was made. Lytic activity was enhanced about 1.5 times by the combination of strain No.5 enzyme and chitinolytic strain F190 enzyme.
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Report
(3 results)
Research Products
(14 results)
