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Expression and Immunocytochemistry of Tyrosine Hydroxylase Subtype mRNA

Research Project

Project/Area Number 01570035
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionFujta Health University, School of Medicine

Principal Investigator

NAGATSU Ikuko  Fujita Health Univ., School of Med., Dept. of Anatomy, Professor, 医学部・解剖学, 教授 (80084573)

Co-Investigator(Kenkyū-buntansha) SAKAI Masao  Fujita Health Univ., School of Med., Dept. of Anatomy, Assistant, 医学部解剖学, 助手 (90196048)
YAMADA Keiki  Fujita Health Univ., School of Med., Dept. of Anatomy, Assistant Prof., 医学部解剖学, 教授 (60084604)
唐沢 延幸  藤田学園保健衛生大学, 医学部・解剖学教室, 研究技術員 (70148287)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordstyrosine hydroxylase / immunocytochemistry / phenylethanolamine-N-methyltransferase / subtype / mRNA / expression
Research Abstract

(1) Our coworkers in T. Nagatsu's laboratory have systematically isolated cDNA and genomic clones encoding catecholamine-synthesizing enzymes. In human tyrosine hydroxylase (hTH), four types of mRNA were produced by alternative RNA splicing from a single gene. The cDNA probe (probe 1 : 0.25kb SacI-EcoRI), which hybridized only with hTHmRNA, or the cDNA probe (probe 2 : 1.3kb EcoRI-SacI), which hybridized with both human and mouse mRNAs was labeled with [a-35S] dCTPaS by the multiprime DNA labeling method. Using probe 2, TH expression was observed in the ventral tegmental area (VTA) and substantia nigra (SN), but no expression was found using probe 1 (manuscript in preparation).
(2) We employed an 11 kb DNA fragment of the TH gene consisting of 2.5 kb of 5'-upstream region containing promoter information, the entire exon-intron structure, and 0.5 kb of the 3'-franking region for the production of transgenic (Tg) mice. Using 35S-labeled protein-coding region of hTHcDNA as a probe (probe 2 … More ), extremely high-level expression of the mRNA was observed in the SN and VTA from Tg mice. Using 35S-labeled hTH-specific cDNA fragment (probe 1), specific expression of hTHmRNA was observed in Tg brain, whereas no hybridization signal was observed in non-Tg brain sections (Ref. 6).
(3) We have described tissue-specific expression of the transgene in catecholaminergic (CAergic) neurons and adrenal glands. In addition to these hTH gene expression in CAergic regions, we also found atypical hTH gene expression in non-catecholaminergic (nCAergic) regions of Tg mouse brain by immunocytochemistry at light and electron microscopic levels and by in situ hybridization. These cells were AADC-, DBH-, PNMT-, DA-, and 5HT-negative. We have previously reported the transient expression of TH in the anterior olfactory nucleus (AON) during late embryonic and postnatal stages (Ref. 3). Moreover, we reported an existence of only PNMT-positive neurons in the AON from postnatal 10 days to adult stage. At electron microscopic level, synaptic formation was observed (Ref. 1). Transient appearance of TH or PNMT immunoreactive neurons was found (Ref. 5).
(4) We succeeded to produce antibodies raised to the different oligopeptide segments of human DBH (Ref. 4). Less

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Nagatsu I.,Komori K.,Miura K.,Sakai M.,Karasawa N.and Yamada K.: "Ontogeny of phenylethanolamineーNーmethyltransferaseー and tyrosine hydroxylaseーimmunoreactive expression in the mouse anterior olfactory nucleus." Biomedical Res.10ーSuppl.3. 277-286 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Karasawa N.,Yoshida M.,Sakai M.,Teramura M.and Nagatsu I.: "Immunohistocytochemical studies on taurineー and GABAーlike immunoreactive structures in the rat cerebellum." Biogenic Amines. 6. 233-240 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagatsu I.,Komori K.,Takeuchi T.,Sakai M.,Yamada K.and Karasawa N.: "Transient tyrosine hydroxylaseーimmunoreactive neurons in the region of the anterior olfactory nucleus of preー and postnatal mice do not contain dopamine." Brain Res.511. 55-62 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagatsu I.,Kobayashi K.,Fujii T.,Komori K.,Sekiguchi K.,titani K.,Fujita K., and Nagatsu T.: "Antibodies raised to the different oligopeptide seguments of human dopamineーΒーhydroxylase." Neurosci.Lett.120. 141-145 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Komori K.,Sakai M.,Karasawa N.,Yamada K. and Nagatsu I.: "Transient expression of tyrosine hydroxylase or phenylethanolamineーNーmethyltransferase immunoreactivity in some neurons of the mouse caudate nucleus during postnatal development." Acta Histochem Cytochem.(1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kaneda N.,Sasaoka T.,Kobayashi k.,Kiuchi K.,Nagatsu I.,Kurosawa Y.,Fujita K.,Yokoyama M.,nomura T.,Katsuki M.and Nagatsu T.: "Tissueーspecific and highーlevel expression of human tyrosine hydroxylase gene in transgenic mice." Neuron. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagatsu I.,Sakai M.,Miura K.and Watanabe K.: "Dopamine immunocytochemistry in the ingrostriatal system of preー and postanal mice.Alzheimer's and Parkinson's Diseases." Plenum Pub.,New York, (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] 永津 郁子: "免疫組織化学。 原理、 伝達物質と関連酵素。 "新生化学実験講座" 神経生化学(永津俊治、 芳賀達也、 御子柴克彦、 和田 博" 東京化学同人、 東京, 88-95 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ichitani, Y., Morimoto, N., Okuyama, I., Hasagawa, M., Nagatsu, I., Ibata, Y.: "Fine structure of tyrosine hydroxylase-like immunoreactive neurons and terminals of the hypothalamic arcuate nucleus and median eminence in young and aged rats" J. Electron Microsc.39. 396-403 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kaneko, T., Akiyama, H., Nagatsu, I., Mizuno, N.: "Immunohistochemical demonstration of glutaminase in catecholaminergic and serotoninergic neurons of rat brain" Brain Res.507. 151-154 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kitahama, K., Geffard, M., Okamura, H., Nagatsu, I.: "Dopamine- and DOPA-immunoreactive neurons in the cat forebrain with reference to tyrosine hydroxylase-immunohistochemistry" Brain Res.518. 83-94 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Komori, K., Miura, K., Takeuchi, T., Sakai, M., Karasawa, N., Nagatsu, I.: "Transient expression of phenylethanolamine-N-methyltransferase immunoreactivity in some neurons of the mouse caudate nucleus during postnatal development" Biogenic Amines. 7. 493-504 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Kubo. K., Onozuka, M., Karasawa, N., Nagatsu, I., Deura, S.: "Immunohistocytochemical and electrophysiological studies on the neuronal reorganization in the cerebellum of Jaundiced Gunn rat" Biogenic Amines. 7. 591-602 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Miura, K., Sakai, M., Komori, K., Furune, S., Watanabe, K., Nagatsu, I.: "Semiquantitative immunohistochemical study on normal development of nigrostriatal dopaminergic neurons in the mouse brain" Biogenic Amines. 7. 483-491 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagatsu, I., Kobayashi, K., Fujii, T., Komori, K., Sekiguchi, K., Titani, K., Fujita, K., Nagatsu, T.: "Antibodies raised to the different oligopeptide segments of human dopamine-B-hydroxylase" Neurosci. Lett.120. 141-145 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagatsu, I., Komori, K., Takeuchi, T., Sakai, M., Yamada, K., Karasawa, N.: "Transient tyrosine hydroxylase-immunoreactive neurons in the region of the anterior olfactory nucleus of pre- and postnatal mice do not contain dopamine" Brain Res.511. 55-62 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Niijima, K., Araki, M., Ogawa, M., Nagatsu, I., Sato, F., Kimura, H., Yoshida, M.: "Enhanced survival of cultured dopamine neurons by treatment with soluble extracts from chemically defferentiated striatum of adult rat brain" Brain Res.528. 151-154 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nonaka, T., Araki, M., Kimura, H., Nagatsu, I., Satoh, F., Masuzawa, T.,: "Transplantation of the rat pineal organ to the grain ; Pinealocyte differentiation and innervation" Cell Tissue Res.260. 273-278 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okamura, H., Kitahama, K., Raynaud, B., Borri-Voltattornic, C., Nagatsu, I., Weber, M.: "Immunocytochemistry of aromatic L-amino acid decarboxylase in the rat preoptic area and anterior hypothalamus, with special reference to tyrosine hdyroxylase immunocytochemistry" Biogenic Amines. 7. 351-361 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Onozuka, M., Kubo, K., Deura, S., Karasawa, N., Nagatsu, I.: "Modification of noradrenaline innervation in the cerebellum of mutant rats with Purkinje cell degeneration (jaundiced Gunn rats)" Neurosci. Res.9. 140-147 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Sakai, M., Ashihara, M., Nishimura, T., Nagatsu, I.: "Carnosine-like immunoreactivity in human olfactory mucosa" Acta Otolaryngologica. 109. 450-453 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tashiro, Y., Kaneko, T., Nagatsu, I., Kikuchi, H., Mizuno, N.: "Increase of tyrosine hydroxylase-like immunoreactive neurons in the nucleus accumbens and the olfactory bulb in the rat with the lesion in the ventral tegmental area of the midbrain" Brain Res.531. 159-166 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tsurusaki, M., Yoshida, M., Akasu, T., Nagatsu, I.: "a2-Adrenoceptors mediate the inhibition of cholinergic transmission in parasympathetic ganglia of the rabbit urinary bladder" Synapse. 5. 233-240 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Ueyama, Y., Kozuki, K., Houtani, T., Ikeda, M., Kitajiri, M., Yamashita, T., Kumazawa, T., Nagatsu, I., Sugimoto, T.: "Immunolocalization of tyrosine hydroxylase and vasoactive intestinal polypeptide in nerve fibers innervating human palatine tonsil and paratonsillar glands" Neurosci. Lett.116. 70-74 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Watanabe, Y., Morikami, T., Nagatsu, I.: "Immunohistochemical colocalization of glucagon, serotonin, and aromatic L-amino acid decarboxylase in islet A cells of chicken pancreas" Cell Tissue Res.259. 67-72 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Yamada, K., Karasawa, N., Fujii, T., Isomura, G., Takagi, I., Sakai, M., Nagatsu, I.: "Immunocytochemical localization of amines and peptides in adrenal chromaffin cells of the house shrew (Suncus murinus)" Biogenic Amines. 7. 223-234 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Nagatsu I.,Komori K.,Miura K.,Sakai M.,Karasawa N.and Yamada K.: "Ontogeny of phenylethanolamineーNーmethyltransferaseーand tyrosine hydroxylaseーimmunoreactive expression in the mouse anterior olfactory nucleus." Biomedical Res.10suppl.3. 277-286 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Karasawa N.,Yoshida M.,Sakai M.,Teramura M.and Nagatsu I.: "Immunohistocytochemical studies on taurineーand GABAーlike immunoreactive structures in the rat cerebellum." Biogenic Amines. 6. 233-240 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagatsu I.,Komori K.,Takeuchi T.,Sakai M.,Yamada K.and Karasawa N.: "Transient tyrosine hydroxylaseーimmunoreactive neurons in the region of the anterior olfactory nucleus of preーand postnatal mice do not contain dopamine." Brain Res.511. 55-62 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagatsu I.,Kobayashi K.,Fujii T.,Komori K.,Sekiguchi K.,Titani K.,Fujita K.,and Nagatsu T.: "Antibodies raised to the different oligopeptide seguments of human dopamineーβーhydroxylase." Neurosci.Lett.120. 141-145 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Komori K.,Sakai M.,Karasawa N.,Yamada K.and Nagatsu I.: "Transient expression of tyrosine hydroxylase or phenylethanolamineーNーmethyltransferase immunoreactivity in some neurons of the mouse caudate nucleus during postnatal development." Acta Histochem Cytochem.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Kaneda N.,Sasaoka T.,Kobayashi K.,Kiuchi K.,Nagatsu I.,Kurosawa Y.,Fujita K.,Yokoyama M.,Nomura T.,Katsuki M.and Nagatsu T.: "Tissueーspecific and highーlevel expression of human tyrosine hydroxylase gene in transgenic mice." Neuron. (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Nagatsu I.,Sakai M.,Miura K.and Watanabe K.: "Dopamine immunocytochemistry in the nigrostriatal system of preーand postnatal mice.Alzheimer's and Parkinson's Diseases." Plenum Pub.,New York, (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] 永津 郁子: "免疫組織化学。原理、伝達物質と関連酵素。“新生化学実験講座"神経生化学(永津俊治、芳賀達也、御子柴克彦、和田博)" 東京化学同人、東京, 88-95 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Furune S.,Miura K.,Watanabe K.,Nagao S.,Takahashi H.,Sakai M.,Spatz M.and Nagatsu I.: "Transplacental effect of 1-methyl-4phenyl-1,2,3,6-tetrahydropyridine(MPTP) on brain dopaminergic neurons in the mouse:an immunohistochemical study." Acta Neuropathologica. 79. 279-285 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nagatsu I.,Furune S.,Ichinose H.,Spatz M.and Nagatsu T.: "Quantitative analysis of reduction of aromatic-L-aminoacid decarboxylase-and tyrosine hydroxylase-like immunoreactivities in the nigrostriatal dopaminergic neurons of MPTP-treated mice." Biogenic Amines. 6. 263-277 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Sakai M.,Kani K.,Yoshida M.and Nagatsu I.: "The dopaminergic cells in the superior cervical ganglion of the rat:a light and electron microscopical stydy using an antibody against dopamine." Neurosci.Lett.96. 157-162 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nagatsu I.,Komori K.,Miura K.,Sakai M.,Karasawa N.and Yamada.K.: "Ontogeny of phenylethanolamine-N-methyltransferase-and tyrosine hydroxylase-immunoreactive exprssion in the mouse anterior olfactory nucleus." Biomedical Res.(1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kaneko T.,Akiyama H.,Nagatsu I.and Mizuno N.: "Immunohistochemical demonstration of glutaminase in catecholaminergic and serotoninergic neurons of rat brain." Brain Res.507. 151-154 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nagatsu I.,Komori K.,Takeuchi T.,Sakai M.,Yamada K.and Karasawa N.: "Transient tyrosine hydroxylase-immunoreactive neurons in the region of the anterior olfactory nucleus of pre-and postnatal mice do not contain dopamine." Brain Res.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Nagatsu I.,Sakai M.,Miura K.and Watanabe K.: "Dopamine immunocytochemistry in the nigrostriatal system of pre-and postnatal mice.Alzheimer's and Parkinson's Doseases." Plenum Pub.,New York, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 永津郁子: "免疫組機化学。原理、伝達物質と関連酵素。"新生化学実験講座"神経生化学 Vol.11," (永津俊治、)東京化学同人、東京, (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 永津郁子,唐沢延幸,山田敬喜,高木勇: "副腎"組織学ー組織化学的アプロ-チ"" (小川和朗,永田哲士,安田健次郎)朝倉書店,東京, (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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