Patch-clamp studies on the membrane properties of granulocytes and their changes during cell-activation.
| Project/Area Number |
01570038
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Gunma University |
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Principal Investigator |
KAWA Kazuyoshi Gunma University School of Medicine Dept Pharmacology, Lecturer, 医学部, 講師 (70125839)
飯野 昌枝 (1990) 群馬大学, 医学部, 助手 (20008329)
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| Co-Investigator(Kenkyū-buntansha) |
IINO Masae Gunma University School of Medicine Dept 2nd Physiology, Assistant Professor, 医学部, 助手 (20008329)
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| Project Period (FY) |
1989 – 1990
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| Project Status |
Completed (Fiscal Year 1990)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1989: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Patch-clamp / Neutrophil / K^+ -channel / Granulocytes / Endocrine cells / イオンチャネル |
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| Research Abstract |
The blood obtained from the atria of the newts (Triturus pyrrhogaster) contains several types of leukocytes. Under a phase contrast microscope, three types of granulocytes could be identified. Using patch-electrodes, it was possible to investigate the whole-cell membrane currents in these cells. In the neutrophils (diameter, 19-23 mum), identified by characteristic features of lobulated nucleus, inward rectifying K^+ currents were evoked with hyperpolarization from the holding potential of -83 mV. In the presence of Cs^+ (1 mM) or Ba^<2+> (1 mM) in the external saline, the amplitudes of inward currents were reduced with time- and voltage- dependent manner. When the external concentration of K^+ was increased from 4 mM through 20 mM to 100 mM replacing isomolar choline in the perfusing salines, the threshold of the K^+ currents shifted from -83 mV through -43 mV to -3 mV.
The amplitude of the evoked K^+ currents also increased as the chord conductance in each of these salines was nearly proportional to the square root of the external K^+ concentration.
In basophils and eosinophils, there evoked no major membrane currents under voltage-clamp conditions. Their I-V relations were almost linear, which suggested almost electrically non-excitable properties in these cells. These electrophysiological properties of granulocytes were compared with those of endocrine cells.
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Report
(3 results)
Research Products
(15 results)
