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A Role of Subcellular Factors to Control Channels in Kidney Tubules.

Research Project

Project/Area Number 01570040
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionUniv. of Tokyo

Principal Investigator

KAWAHARA Katsumasa  Univ. of Tokyo, Physiol. Sci. Ass., 医学部(医)・第二生理, 助手 (70134525)

Co-Investigator(Kenkyū-buntansha) SUZUKI Makoto  Jikei Univ. School of Med. Sci. Ass., 第二内科, 助手 (10196868)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPatch-clamp / Ion channel / Cell Volume Regulation / Membrane Stretch / Ca / G proteins / パッチクランプ法 / 細胞増殖因子 / GTPγS
Research Abstract

Using the patch-clamp technique, we investigated a mechanism of activation of single-channel currents during cell volume regulation in cultured rabbit kidney proximal tubule cells. Hypotonically induced cell-swelling increased Ca-dependent K currents, and then, Cl currents. Because cell membrane potential initially hyperpolarized, and then returned to the original level with KCl in the pipette (current-clamp mode). Omitting extracellular Ca from and adding 0.1 mM EGTA to the bath solution significantly slowed a time course of cell volume regulation. At Vp=0 mV the whole-cell K currents induced by hypotonicity decreased by 90% when a pipette-filling solution contained zero Ca and 5 mM EGTA. Addition of 5 mM Ba to the bath almost completely inhibited K currents due to cell-swelling. Activation of Cl currents due to hypotonicity was partially inhibited by either 20 uM SITS in the bath or zero Ca plus 5 mMEGTA in the pipette(whole-cell clamp condition).
In conclusion, hypotonicallyinduced cell swelling stimulates Ca-activated K channels, which may play an important role in cell volume regulation. A secondary activation of Cl currents may also be essential for the regulatory volume decrease mechanism.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report

Research Products

(16 results)

All Other

All Publications (16 results)

  • [Publications] KAWAHARA,K.: "A stretchーactivated K^+ channel in the basolcteral membrane of xenopus Kidney proximal tubule cells." Ptlugers Ard Europedn J.of Physiol.415. 624-629 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KAWAHARA,K.: "Hyposmotic dctivation of Cdーactivated K channels in cultured rabbit Kidney proximal tubule cells" Am.J.Physiol. 260. F27-F33 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KAWAHARA,K.: "The Frontiers of Nephrolgy" Excerptd Medidca, 254 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KAWAHARA, K.: "A Stretch-Activated K Channel in the Basolateral Membrane of Xenopus Kidney Proximal Tubule Cells." Pfluegers Arch. 415. 624-629 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KAWAHARA, K.: "Hyposmotic Activation of Ca-Activated K Channels in Cultured Rabbit Kidney Proximal Tubule Cells." Am. J. Physiol.260. 27-33 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KAWAHARA, K.: "Regulatory Volume Decrease Mechanism in Cultured Rabbit Kidney Proximal Tubule Cells." The Frontiers of Nephrology Elsevier. Amsterdam. 13-20 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] KAWAHARA,K.: "A stretchーactivated K^+ channel in the basolateral membrance of Xenopus kindney proximal tubule cells." Pflu^^¨gers Arch European J.of Physiol.415. 624-629 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] KAWAHARA,K.: "Hyposmotic activation of Caーactivated K channels in cultured rabbit kidney proximal tubule cells" Am.J.Physiol.260. F27-F33 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] KAWAHARA,K.: "The Frontiers of Nephrology" Excerpts Medica, 254 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Giebisch,G.: "Apical potassium channels in Amphiuma diluting segment:Effect of barium" J.Physiology(Lond.). 420. 313-323 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kawahara,K.: "Stretch-activated K channel in basolateral membrane of Xenopus kidney proximal tubule cells" Pfluegers Arch.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kawahara,K.: "“Flickery block"of Ca-activated K channels by intracellular Na in the luminal membrane of Amphiuma diluting segment" Pfluegers Arch.(1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Suzuki,M.: "G-protein is involved in volume regulation of rabbit renal proximal convoluted cells in culture" Am.J.physiology. (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] 河原克雅: "パッチクランプ法" 日本臨床. 47. 1460-1465 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] 河原克雅: "腎におけるイオンチャネル" 腎と透析. 26. 553-555 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Kawahara,K.: "The Frontiers of Nephrology(eds.Berliner,Ullich,& Honda)" Elsevier, 300 (1990)

    • Related Report
      1989 Annual Research Report

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Published: 1989-03-31   Modified: 2016-04-21  

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