• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Inositol 1,4,5,-triphosphate activated Calcium Channels in oocytes.

Research Project

Project/Area Number 01570043
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionNagoya University

Principal Investigator

KURODA Hideyo  Nagoya Univ.,Sch. of Sci., Assoc. Prof., 理学部, 助教授 (50064845)

Co-Investigator(Kenkyū-buntansha) SOKABE Masahiro  Nagoya Univ., Sch. of Med., Assoc. Prof., 医学部, 助教授 (10093428)
SATO Hidemi  Nagoya Univ. Sch. of Sci. Prof., 理学部, 教授 (40109260)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1989: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsIP_3 / IP_3 receptor / calcium release / calcium channel / sea urchin egg / fertilization / イノシト-ルミりん酸 / イノシト-ル三りん酸
Research Abstract

A transient increase in intracellular calcium concentration occurs in sea urchin eggs during fertilization. Inositol 1,4,5,-triphosphate(IP_3) is thought to be the second messenger which activates calcium channels of intracellular store and to cause the Ca-transient. However, there is no direct evidence for such a IP_3-activated Ca channel in sea urchin eggs. These experiments aimed to show the evidences.
1. IP_3 caused a transient release of Ca^<2+> from microsomal fraction of unfertililized eggs. The release was inhibited by the application of heparin (1 mg/ml). We found in addition, that caffein also caused a transient release of Ca^<2+> from the microsomal fraction in dose dependent manner. Heparin did not block the release. This means that caffeine causes a Ca release via a different mechanism from the IP_3-induced Ca release.
2. We tried to measured the current passing a IP_3-induced Ca releasing channel. Single channel recordings of the microsomal membrane which fused with a planar bilayer membrane were examined. Several kinds of Ca^<2+>, K^+ and Cl^- channels were found but no IP_3-induced Ca channel was observed.
3. Another approach to show the existence of IP_3- induced Ca releasing channel must be the purification of "IP_3-receptors" from the microsomal fraction. IP_3 binding proteins of about 200 KD were obtained. The reconstitution of the protein into an artificial membrane is now examining.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] R.Kuroda,H.Kuroda,T.Murai and Y.Imae: "Purification ofinositol 1,4,5,-triphosphate receptors from sea urchin eggs." Dev.Biol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] R.Kuroda, H.Kuroda, T.Murai and Y.Imae.: "Purification of inositol 1,4,5,-triphosphate receptors from sea urchin eggs." Dev. Biol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] H.Soga,S.Takeda,T.Soga,R.Kuroda and H.Kuroda: "Inositol triphosphateーstimulated Ca^<2+> release from microsome fractions of sea urchin eggs." Biochim.Biophys.Acta.

    • Related Report
      1990 Annual Research Report
  • [Publications] Obata,S.and Kuroda,H.: "Membrane potential change in a sea urchin egg induced by calcium ionophore(A23187)has the same component that is involved in the fertilization potential." Cell Struct.Funct.14. 697-706 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Obata,S.and Kuroda,H.: "Voltage-independent component of the fertilization current in sea urchin eggs." Submitted to Dev.Biol.

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi