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The molecular mechanism of electron transfer in mitochondria beta-oxidation

Research Project

Project/Area Number 01570046
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionKumamoto University

Principal Investigator

NISHINA Yasuzo  Kumamoto University Medical School, Assoc. Prof., 医学部, 助教授 (50112553)

Co-Investigator(Kenkyū-buntansha) SATO Kyosuke  Kumamoto University Medical School, Joshu, 医学部, 助手 (80205905)
SHIGA Kiyoshi  Kumamoto University Medical School, Prof., 医学部, 教授 (40028527)
Project Period (FY) 1989 – 1991
Project Status Completed (Fiscal Year 1991)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1991: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1990: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1989: ¥900,000 (Direct Cost: ¥900,000)
KeywordsFlavoprotein / Raman Spectra / Acyl-CoA / FAD / アシル-CoA / 光散乱
Research Abstract

(1) Resonance Raman (RR) spectra of the complex of medium-chain acyl-CoA dehydrogenase with acetoacetyl-CoA and of the purple complex formed upon the addition of octanoyl-CoA to the dehydrogenase were obtained. The RR results confirm that the purple complex is a charge-transfer complex between the reduced enzyme and octenoyl-CoA derived from octanoyl-CoA. The large contribution of ionic resonance structure, C(2)=C(l)-O^-, for the ligands in both complexes, can be explained by the existence of an electrophilic group near the 0 atom of the C(l)=O group of the ligands and the electrostatic interaction between the group and the 0 atom of. ligand. This electrostatic interaction probably lowers the pKa value of a substrate at C(2)-H in an early step of reductive half reaction, facilitating the abstraction of alpha-proton.
(2) The association process of FAD and apo-electron-transferring flavoprotein (apoETF) was investigated. Kinetic studies suggest the existence of two different forms of apoETF, as represented in the following reaction scheme : A^* A, A + FAD HoloETF, where "A^*" and "A" are the different forms of apoETF. Anions induced the conversion from"A^*' to "A" ; the order of the effectiveness was I^- - Br^- > Cl^- > F^-. Glycerol also induced the conversion. The elution pattern of apoETF on a molecular sieve chromatography was changed by addition of salts or glycerol ; this change was due to the conversion from 'A^*" to "A" by the added solutes. "A^*" form was eluted out more rapidly than "A" form, indicating that "A^*" form exists in a relatively loose conformation than "A" form. The far-UV CD studies support this conclusion.

Report

(4 results)
  • 1991 Annual Research Report   Final Research Report Summary
  • 1990 Annual Research Report
  • 1989 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Y.Nishina: "proton release from flaroprotein D-amino acid oxidase on complexation with the zwitterionic ligand,trigonelline" J.Biochem.107. 726-731 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Sato: "The existence of two different forms of apo-electron-transferring flavoprotein" J.Biochem.109. 734-740 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Nishina: "isomerization of Δ^1-piperideine-2-carboxylate to Δ^2-piperideine-2-carboxylate on complexation with flavoprotein D-amino acid axidase" J.Biochem.109. 705-710 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Suzuki: "A resonance Raman study on a reaction intermediate of P seudomonas L-phenylalanine oxidase(deaminating and decarboxylating)" J.Biochem.110. 169-172 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K.Sato: "Anion-induced conformational change of apo-electron-transferring flavoprotein" J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y.Nishina: "Resonance Raman study on complexes of medium-chain-acyl-CoA dehydrogenase" J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Nishina: ""Proton release from flavoprotein D-amino acid oxidase on complexation with trigonelline"" J. Biochem.107. 726-731 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Nishina: ""Isomerization of DELTA^1-piperideine-2-carboxylate to DELTA^2-piperideine-2-carboxylate on complexation with flavoprotein D-amino acid" J. Biochem.109. 703-710 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Sato: ""The existence of two different forms of apo-electron-transferring flavoprotein"" J. Biochem.109. 734-740 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H. Suzuki: ""A resonance Raman study on a reaction intermediate of Pseudomonas L-phenylalanine oxidase (deaminating and decarboxylating)"" J. Biochem.110. 169-172 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] K. Sato: ""Anion-induced conformational change of apo-electron transferring flavoprotein"" J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] Y. Nishina: ""Resonance Raman study on complexes of medium-chain acyl-CoA dehydrogenase"" J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1991 Final Research Report Summary
  • [Publications] H.Suzuki: "A resonance Raman study on a reaction intermediate of Pseudomonas Lーphenylalanine oxidase(decmihatiny and decarboxylating)" J.Biochem.110. 169-172 (1991)

    • Related Report
      1991 Annual Research Report
  • [Publications] K.Sato: "Anionーinduced conformational change of apoーelectronーtransferring flavoprotein" J.Biochem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Nishina: "Resonance Raman study on complexes of mediumーchain acylーCoA dehydrogenase" J.Biochem.

    • Related Report
      1991 Annual Research Report
  • [Publications] Y.Nishina: "Structural change of a ligand upon complexation with Dーamino acid oxidase" Flavins and Flavoproteins,.

    • Related Report
      1990 Annual Research Report
  • [Publications] K.Sato: "The existence of two different forms of apoーelectronーtransferring flavoprotein" J.Biochem.

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nishina: "Isomerization of Δ^1ーpiperideineー2ーcarboxylate to Δ^2ーpiperideineー2ーcarboxylate on complexation with flavoprotein Dーamino acid oxidase" J.Biochem.

    • Related Report
      1990 Annual Research Report
  • [Publications] Y.Nishina: "Proton release from flavoprotein D-amino acid oxidase on complexation with the zwitterionic ligand trigonelline" J.Biochem.

    • Related Report
      1989 Annual Research Report
  • [Publications] K.Sato: "A study of the association-dissociation equilibrium of subunits of electron-transferring flavoprotein" Jpn.J.physiol.39. S16 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Y.Nishina: "Control by ligand binding of reaction between semiquinoid form of flavoprotein D-amino acid oxidase and molecular oxygen" Jpn.J.Physiol.39. S16 (1989)

    • Related Report
      1989 Annual Research Report

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Published: 1989-04-01   Modified: 2016-04-21  

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