• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the Transport and Localization of Na, K-ATPase

Research Project

Project/Area Number 01570050
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field General physiology
Research InstitutionKansai Medical University

Principal Investigator

OMORI Koichiro  Kansai Med. Univ., Dpt. of Physiol., Assistant Prof., 医学部 (80094465)

Co-Investigator(Kenkyū-buntansha) TASHIRO Yutaka  Kansai Med. Univ., Dpt. of Physiol., Professor, 医学部, 教授 (40077558)
YOSHIMORI Tamotsu  Kansai Med. Univ., Dpt. of Physiol., Assistant, 医学部, 助手 (60191649)
YAMAMOTO Akitsugu  Kansai Med. Univ., Dpt. of Physiol., Lecturer, 医学部, 講師 (30174775)
Project Period (FY) 1989 – 1990
Project Status Completed (Fiscal Year 1990)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1990: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1989: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsNa, K-ATPase / protein biosynthesis / immunohistochemistry / cochlear duct / ciliary epithelial cell / retinal pigment epithelial cell / clathrin-coated vesicles / Na,K-ATPase / DNAトランスフェクション / サブユニットアセンブリング / HeLa細胞
Research Abstract

Na, K-ATPase consists of the alpha and the beta subunit, with the stoichiometry of one to one. The alpha subunit contains the binding site for ouabain as well as the catalytic site. The beta subunit is a glycosylated membrane protein with unknown function.
Recently, several studies have suggested the functional roles of the beta subunit in the maturation and/or transport processes of the enzyme. It is well known that the enzyme exclusively distributes on the basolateral plasma membrane. Some mechanisms should exist for the localization of the enzyme on the plasma membrane. Furthermore, it comes to be evident that the enzyme is endocytosed from the plasma membrane and affects the internal pH of the endosome.
On the bases of these findings, we tried to elucidate the mechanisms of the transport and localization of the enzyme, and obtained the following results.
(1) We expressed the N-terminal half of the beta sububit of rat brain Na, K-ATPase in HeLa cells, and observed that the expressed fragment protein was localized in the ER and inhibited the assembly of the endogenous alpha and beta subunits. These results suggests that the N-terminal portion of the beta subunit interferes with the assembly of the endogenous complex which takes place in the ER.
(2) We quantitatively analyzed the distribution of the enzyme by using immunoelectron microscpy in rat cochlear duct, ciliary epithelial cells and retinal pigment epithelial cells.
(3) we isolated and discriminated two types of clathrin-coatrd vesicles originated from neuronal and glial cells, respectively. The clathrin-coated vesicles from neuronal cells dominantly contained alpha+ subunit which is characteristic for neuronal cells.

Report

(3 results)
  • 1990 Annual Research Report   Final Research Report Summary
  • 1989 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Tadashi Iwano: "Ωuantitative immunocytochemical localization of Na^+,K^+ーATPase αーsubunit in the lateral wall of rat cochlear duct." J.Histochem.Cytochem.37. 353-363 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toyokazu Okami: "Ωuantitative immunocytochemical localization of Na^+,K^+ーATPase in rat ciliary epithelial cells." J.Histochem.Cytochem.37. 1353-1361 (1989)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Iwano: "Ωuantitative immunogold localization ofNa^+,K^+ーATPase αーsubunit in the tympanic wall of rat cochlear duct." J.Histochem.Cytochem.38. 225-232 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toyokazu Okami: "Immunocytochemical localization of Na^+,K^+ーATPase in rat retinal pigment epitelial cells." J.Histochem.Cytochem.38. 1267-1275 (1990)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Koichiro Omori: "Expression,localization,and function of an Nーterminal half fragment of the rat Na,KーATPase βーsubunit in HeLa cells." J.Biochem.109. 267-275 (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Toshiko Uriu: "Two types of clathrinーcoated vesicles isolated from rat brain: analysis of biochemical properties and cellular origin." J.Neurochem.56. (1991)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Iwano, T., Yamamoto, A., Omori, K., Akayama, M., Kumazawa, T., and Tashiro, Y.: "Quantitative immunocytochemical localization of Na^+, K^+-ATPase alpha-subunit in the lateral wall of rat cochlear duct." J. Histochem. Cytochem.37. 353-363 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okami, T., Yamamoto, A., Omori, K., Akayama, M., Uyama, M., and Tashiro, Y.: "Quantitative immunocytochemical localization of Na^+, K^+-ATPase in rat ciliary epithelial cells." J. Histochem. Cytochem.37. 1353-1361 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Iwano, T., Yamamoto, A., Omori, K., Kawasaki, K., Kumazawa, T., and Tashiro, Y.: "Quantitative immunogold localization of Na^+, K^+-ATPase alpha-subunit in the tympanic wall of rat cochlear duct." J. Histochem. Cytochem.38. 225-232 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Okami, T., Yamamoto, A., Omori, K., Takada, T., Uyama, M., and Tashiro, T.: "Immunocytochemical localization of Na^+, K^+-ATPase in rat retinal pigment epithelial cells." J. Histochem. Cytochem.38. 1267-1275 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Omori, K., Omori, K., Morimoto, T., Takada, T., Akayama, M., Yoshimori, T., Sabatini, D. D., and Tashiro, Y.: "Expression, localization, and function of an N-terminal half fragment of the rat Na, K-ATPase beta-subunit in HeLa cells." J. Biochem.109. 267-275 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Uriu, T., Omori, K., Omori, K., Yamamoto, A., Inoue, M., and Inagaki, C.: "Two types of clathrin-coated vesicles isolated from rat brain : analysis of biochemical properties and cellular origin." J. Neurochem.56. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1990 Final Research Report Summary
  • [Publications] Tadashi Iwano: "Quantitative immunocytochemical localization of Na^+,K^+ーATPase αーsubunit in the lateral wall of rat cochlear duct." J.Histochem.Cytochem.37. 353-363 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toyokazu Okami: "Quantitative immunocytochemical localization of Na^+,K^+ーATPase in rat ciliary epithelial cells." J.Histochem.Cytochem.37. 1353-1361 (1989)

    • Related Report
      1990 Annual Research Report
  • [Publications] Tadashi Iwano: "Quatitative immunogold localization of Na^+,K^+ーATPase αーsubunit in the tympanic wall of rat cochlear duct." J.Histochem.Cytochem.38. 225-232 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toyokazu Okami: "Immunocytochemical localization of Na^+,K^+ーATPase in rat retinal pigment epithelial cells." J.Histochem.Cytochem.38. 1267-1275 (1990)

    • Related Report
      1990 Annual Research Report
  • [Publications] Koichiro Omori: "Expression,localization,and function of an Nーterminal half fragment of the rat Na,KーATPase βーsubunit in HeLa cells." J.Biochem.109. 267-275 (1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Toshiko Uriu: "Two types of clathrinーcoated vesicles isolated from rat brain:analysis of biochemical properties and cellular origin." J.Neurochem.(1991)

    • Related Report
      1990 Annual Research Report
  • [Publications] Koichiro Omori: "Characterization and expression of a cDNA clone for the beta subunit of rat brain Na^+,K^+-ATPase." Prog.Clin.Biol.Res.268B. 127-134 (1988)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadashi Iwano: "Quantitative immunocytochemical localization of Na^+,K^+-ATPase α-subunit in the lateral wall of rat cochlear duct." J.Histochem.cytochem.37. 353-363 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Toyokazu Okami: "Quantitative immunocytochemical localization of Na^+,K^+-ATPase in rat ciliary epithelial cells." J.Histochem.Cytochem.37. 1353-1361 (1989)

    • Related Report
      1989 Annual Research Report
  • [Publications] Tadashi Iwano: "Quantitative immunogold localization of Na^+,K^+-ATPase α-subunit in the tympanic wall of rat cochlear duct." J.Histochem.Cytochem.38. 225-232 (1990)

    • Related Report
      1989 Annual Research Report
  • [Publications] Toshiko Uriu: "Two types of clathrin-coated vesicles in the rat brain:analysis for cellular origin." J.Neurochem.

    • Related Report
      1989 Annual Research Report
  • [Publications] Koichiro Omori: "Expression.localization,and function of a N-terminal half fragment of the rat Na,K-ATPase β-subunit in HeLa cells."

    • Related Report
      1989 Annual Research Report

URL: 

Published: 1989-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi